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Gene |

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Standard Name	Plasmid F traG
Gene Synonym(s)	traG, Fpla101
Product Desc.	traG required for pilus assembly and DNA transfer; this inner membrane protein is bifunctional; the N-terminal domain is required for pilus assembly while the periplasmic C-terminal domain is required for mating pair stabilization; may form a cleavage product, TraG*; plasmid-specific interactions with the entry exclusion protein TraS in the inner membrane of the recipient cell result in decreased transfer efficiency
Product Synonyms(s)	traG, Fpla101
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	Plasmid F traG
Mnemonic
Synonyms	traG, Fpla101
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
NC_002483		NC_002483: 85425..88241 <gbrowseImage> name=NC_002483:85425..88241 source=plasmid_F preset=GeneLocation </gbrowseImage>	Gbrowse (Plasmid F)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	traG
Synonyms	traG, Fpla101
Product description	traG required for pilus assembly and DNA transfer; this inner membrane protein is bifunctional; the N-terminal domain is required for pilus assembly while the periplasmic C-terminal domain is required for mating pair stabilization; may form a cleavage product, TraG*; plasmid-specific interactions with the entry exclusion protein TraS in the inner membrane of the recipient cell result in decreased transfer efficiency
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Status
	GO:0005886	plasma membrane	PMID:17259615	IDA: Inferred from Direct Assay	C	complete
	GO:0009291	unidirectional conjugation	PMID:17259615	IMP: Inferred from Mutant Phenotype	P	complete
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MNEVYVIAGG EWLRNNLNAI AAFMGTWTWD SIEKIALTLS VLAVAVMWVQ RHNVMDLLGW VAVFVLISLL VNVRTSVQII DNSDLVKVHR VDNVPVGLAM PLSLTTRIGH AMVASYEMIF TQPDSVTYSK TGMLFGANLI VKSTDFLSRN PEIINLFQDY VQNCVLGDIY LNHKYTLEDL MASADPYTLI FSRPSPLRGV YDNNNNFITC KDASVTLKDR LNLDTKTGGK TWHYYVQQIF GGRPDPDLLF RQLVSDSYSY FYGSSQSASQ IMRQNVTMNA LKEGITSNAA RNGDTASLVS LATTSSMEKQ RLAHVSIGHV TMRNLPMVQT ILTGIAIGIF PLLILAAVFN KLTLSVLKGY VFALMWLQTW PLLYAILNSA MTFYAKQNGA PVVLSELSQI QLKYSNLAST AGYLSAMIPP LSWMMVKGLG AGFSSVYSHF ASSSISPTAS AAGSVVDGNY SYGNMQTENV NGFSWSTNST TSFGQMMYQT GSGATATQTR DGNMVMDASG AMSRLPVGIN ATRQIAAAQQ EMAREASNRA ESALHGFSSS IASAWNTLSQ FGSNRGSSDS VTGGADSTMS AQDSMMASRM RSAVESYAKA HNISNEQATR ELASRSTNAS LGLYGDAYAK GHLGISVLGN GGGVGLQAGA KASIDGSDLD SHEASSGSRA SHDARHDIDA RATQDFKEAS DYFTSRKVSE SGSHTDNNAD SRVDQLSAAL NSAKQSYDQY TTNMTRSHEY AEMASRTESM SGQMSEDLSQ QFAQYVMKNA PQDVEAILTN TSSPEIAERR RAMAWSFVQE QVQPGVDNTW RESRRDIGKG MESVPSGGGS QDIIADHQGH QAIIEQRTQD SNIRNDVKHQ VDNMVTEYRG NIGDTQNSIR GEENIVKGQY SELQNHHKTE ALTQNNKYNE EKLAQERIPG ADSPKELLEK AKSYQHKE
Length	938
Mol. Wt	102.472 kDa
pI	6.3 (calculated)
Extinction coefficient	122,160 - 122,410 (calc based on 34 Y, 13 W, and 2 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
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<motif_map/>

Structure
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Structures

<beststructure> gene=Plasmid_F_traG taxon=2465 </beststructure>

Models

View models at:

PSI-PRED
Genomes TO Protein (GTOP)

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_002483:85405..85445 source=plasmid_F type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to Plasmid F traG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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Plasmid F traG:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to Plasmid F traG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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