PMID:17259615
| Citation |
Audette, GF, Manchak, J, Beatty, P, Klimke, WA and Frost, LS (2007) Entry exclusion in F-like plasmids requires intact TraG in the donor that recognizes its cognate TraS in the recipient. Microbiology (Reading, Engl.) 153:442-51 |
|---|---|
| Abstract |
The mating pair stabilization (Mps) protein of the F plasmid, TraG, is unique to F-like type IV secretion systems. TraG is a polytopic inner-membrane protein with a large C-terminal periplasmic domain that is required for piliation and Mps, whereas the N-terminal region is sufficient for pilus synthesis. The C-terminal region of TraG is thought to be cleaved by the host signal peptidase I to give a fragment called TraG* that is responsible for Mps. Using mutational analysis and cell localization studies, it was shown that TraG* is most probably an artifact caused by non-specific degradation. TraS (173 aa in F), which is involved in entry exclusion (Eex), blocks redundant conjugative DNA synthesis and transport between donor cells, suggesting that it interferes with a signalling pathway required to trigger DNA transfer. Using the F and R100 plasmids, TraG in the donor cell was found to recognize TraS in the recipient cell inner membrane, in a plasmid-specific manner. This activity mapped to aa 610-673 in F TraG, the only region that differs significantly from R100 TraG. Expression of traG or traG* in a recipient cell did not affect mating ability or Eex. These results suggest that TraG may be translocated to the recipient cell, where it contacts the inner membrane, initiating transfer, a process that is blocked by TraS. |
| Links |
PubMed Online version:10.1099/mic.0.2006/001917-0 |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Conjugation, Genetic; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; F Factor/genetics; F Factor/metabolism; Gene Expression Regulation, Bacterial; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data |
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