Plasmid F traG:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

traG

Synonyms

traG, Fpla101

Product description

traG

required for pilus assembly and DNA transfer; this inner membrane protein is bifunctional; the N-terminal domain is required for pilus assembly while the periplasmic C-terminal domain is required for mating pair stabilization; may form a cleavage product, TraG*; plasmid-specific interactions with the entry exclusion protein TraS in the inner membrane of the recipient cell result in decreased transfer efficiency

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005886

plasma membrane

PMID:17259615[1]

IDA: Inferred from Direct Assay

C

complete

GO:0009291

unidirectional conjugation

PMID:17259615[1]

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MNEVYVIAGG EWLRNNLNAI AAFMGTWTWD SIEKIALTLS VLAVAVMWVQ RHNVMDLLGW
VAVFVLISLL VNVRTSVQII DNSDLVKVHR VDNVPVGLAM PLSLTTRIGH AMVASYEMIF
TQPDSVTYSK TGMLFGANLI VKSTDFLSRN PEIINLFQDY VQNCVLGDIY LNHKYTLEDL
MASADPYTLI FSRPSPLRGV YDNNNNFITC KDASVTLKDR LNLDTKTGGK TWHYYVQQIF
GGRPDPDLLF RQLVSDSYSY FYGSSQSASQ IMRQNVTMNA LKEGITSNAA RNGDTASLVS
LATTSSMEKQ RLAHVSIGHV TMRNLPMVQT ILTGIAIGIF PLLILAAVFN KLTLSVLKGY
VFALMWLQTW PLLYAILNSA MTFYAKQNGA PVVLSELSQI QLKYSNLAST AGYLSAMIPP
LSWMMVKGLG AGFSSVYSHF ASSSISPTAS AAGSVVDGNY SYGNMQTENV NGFSWSTNST
TSFGQMMYQT GSGATATQTR DGNMVMDASG AMSRLPVGIN ATRQIAAAQQ EMAREASNRA
ESALHGFSSS IASAWNTLSQ FGSNRGSSDS VTGGADSTMS AQDSMMASRM RSAVESYAKA
HNISNEQATR ELASRSTNAS LGLYGDAYAK GHLGISVLGN GGGVGLQAGA KASIDGSDLD
SHEASSGSRA SHDARHDIDA RATQDFKEAS DYFTSRKVSE SGSHTDNNAD SRVDQLSAAL
NSAKQSYDQY TTNMTRSHEY AEMASRTESM SGQMSEDLSQ QFAQYVMKNA PQDVEAILTN
TSSPEIAERR RAMAWSFVQE QVQPGVDNTW RESRRDIGKG MESVPSGGGS QDIIADHQGH
QAIIEQRTQD SNIRNDVKHQ VDNMVTEYRG NIGDTQNSIR GEENIVKGQY SELQNHHKTE
ALTQNNKYNE EKLAQERIPG ADSPKELLEK AKSYQHKE
Length

938

Mol. Wt

102.472 kDa

pI

6.3 (calculated)

Extinction coefficient

122,160 - 122,410 (calc based on 34 Y, 13 W, and 2 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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Structure
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Structures

<beststructure> gene=Plasmid_F_traG taxon=2465 </beststructure>

Models

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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  1. ↑ 1.0 1.1 Audette, GF et al. (2007) Entry exclusion in F-like plasmids requires intact TraG in the donor that recognizes its cognate TraS in the recipient. Microbiology (Reading, Engl.) 153 442-51 PubMed

Categories

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