Plasmid F traA:On One Page

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Standard Name

Plasmid F traA

Gene Synonym(s)

traA, Fpla076

Product Desc.

traA

this pilus subunit is processed to propilin and then N-acetylated by TraX; the subunits lying in the inner membrane are assembled to form the pilus, a long extracellular filament that is essential for transfer, contacts the recipient cell and retracts, bring the cells into close contact prior to conjugal DNA transfer

Product Synonyms(s)

traA, Fpla076

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name

Plasmid F traA

Mnemonic
Synonyms

traA, Fpla076

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

NC_002483

NC_002483: 68265..68630
<gbrowseImage> name=NC_002483:68265..68630 source=plasmid_F preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

traAK100A

K100A

Reduced cleavage of propilin to pilin; when associated with A-97

seeded from UniProt:P04737

traAF101A

F101A

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer

seeded from UniProt:P04737

traAF105V

F105V

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer

seeded from UniProt:P04737

traAF111V

F111V

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer

seeded from UniProt:P04737

traAG120D

G120D

Resistant to phages R17 and QB attachment

seeded from UniProt:P04737

traAD58A

D58A

No production of propilin or pilin

seeded from UniProt:P04737

traAG9S

G9S

No production of pili

seeded from UniProt:P04737

traAP13S

P13S

No production of pili

seeded from UniProt:P04737

traAK68A

K68A

No production of propilin or pilin

seeded from UniProt:P04737

traAF71V

F71V

No effect on propilin synthesis. Defective pilus tip assembly

seeded from UniProt:P04737

traAK79A

K79A

No effect on propilin synthesis. Resistant to phage R17 attachment

seeded from UniProt:P04737

traAD74G

D74G

No effect on propilin synthesis. No effect on pilus formation. Resistant to phage R17 attachment

seeded from UniProt:P04737

traAL121S

L121S

No effect on propilin synthesis. Defective pilus tip assembly

seeded from UniProt:P04737

traAA69E

A69E

No effect on propilin synthesis. No effect on pilus formation. Resistant to phage R17 attachment

seeded from UniProt:P04737

traAK68E

K68E

Resistant to phage R17 attachment

seeded from UniProt:P04737

traAG72D

G72D

No effect on propilin synthesis. No effect on pilus formation

seeded from UniProt:P04737

traAK73A,T

K73A,T

No effect on propilin synthesis. No effect on pilus formation

seeded from UniProt:P04737

traAK79I

K79I

No effect on propilin synthesis. Defective pilus tip assembly

seeded from UniProt:P04737

traAV81I

V81I

Slightly reduced phage R17 attachment, reduced ability to retract

seeded from UniProt:P04737

traAE85A,G

E85A,G

No effect on propilin synthesis. Defective pilus tip assembly

seeded from UniProt:P04737

traAY93D

Y93D

Production of small amounts of propilin and pilin

seeded from UniProt:P04737

traAK97A,R,V

K97A,R,V

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse

seeded from UniProt:P04737

traAK97A

K97A

Reduced cleavage of propilin to pilin; when associated with A-100

seeded from UniProt:P04737

traAN98A

N98A

No effect on propilin synthesis. No effect on pilus formation

seeded from UniProt:P04737

traAV99A

V99A

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse

seeded from UniProt:P04737

traAK100A,R,T

K100A,R,T

No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer

seeded from UniProt:P04737

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference
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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

traA

Synonyms

traA, Fpla076

Product description

traA

this pilus subunit is processed to propilin and then N-acetylated by TraX; the subunits lying in the inner membrane are assembled to form the pilus, a long extracellular filament that is essential for transfer, contacts the recipient cell and retracts, bring the cells into close contact prior to conjugal DNA transfer

EC number (for enzymes)


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Notes

Function

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Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009418

pilus shaft

PMID:11849547[1]

IMP: Inferred from Mutant Phenotype

C

complete

GO:0044096

type IV pilus

PMID:11849547[1]

IMP: Inferred from Mutant Phenotype

C

complete

GO:0009291

unidirectional conjugation

PMID:4555413[2]

IGI: Inferred from Genetic Interaction

EcoliWiki:traA


P

complete

GO:0019062

virion attachment to host cell surface receptor

PMID:11849547[1]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0044096

type IV pilus

PMID:8444800[3]

IDA: Inferred from Direct Assay

C

complete

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Interactions

<protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MNAVLSVQGA SAPVKKKSFF SKFTRLNMLR LARAVIPAAV LMMFFPQLAM AAGSSGQDLM
ASGNTTVKAT FGKDSSVVKW VVLAEVLVGA VMYMMTKNVK FLAGFAIISV FIAVGMAVVG L
Length

121

Mol. Wt

12.767 kDa

pI

11.0 (calculated)

Extinction coefficient

6,990 (calc based on 1 Y, 1 W, and 0 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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<motif_map/>

Structure
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Structures

<beststructure> gene=Plasmid_F_traA taxon=2465 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Links

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Expression

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Overview

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Cellular Levels

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Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)
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Notes

Transcription and Transcriptional Regulation

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Notes

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Translation and Regulation of Translation

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<gbrowseImage> name=NC_002483:68245..68285 source=plasmid_F type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies

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Type Reference Notes
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Expression Resources

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Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to Plasmid F traA Expression

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Links

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Evolution

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Homologs in Other Organisms

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Organism Homologs (Statistics) Comments
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Notes

Families

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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  1. ↑ 1.0 1.1 1.2 Manchak, J et al. (2002) Mutational analysis of F-pilin reveals domains for pilus assembly, phage infection and DNA transfer. Mol. Microbiol. 43 195-205 PubMed
  2. ↑ Willetts, N & Achtman, M (1972) Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J. Bacteriol. 110 843-51 PubMed
  3. ↑ Moore, D et al. (1993) The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin. J. Bacteriol. 175 1375-83 PubMed

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