Plasmid F traA:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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<protect>
| Standard Name |
Plasmid F traA |
|---|---|
| Gene Synonym(s) |
traA, Fpla076 |
| Product Desc. |
traA this pilus subunit is processed to propilin and then N-acetylated by TraX; the subunits lying in the inner membrane are assembled to form the pilus, a long extracellular filament that is essential for transfer, contacts the recipient cell and retracts, bring the cells into close contact prior to conjugal DNA transfer |
| Product Synonyms(s) |
traA, Fpla076 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links |
|
| edit table |
</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
Plasmid F traA |
|---|---|
| Mnemonic | |
| Synonyms |
traA, Fpla076 |
| edit table |
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table. </protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
NC_002483 |
NC_002483: 68265..68630 |
|||
| edit table |
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table. </protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki. <protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
traAK100A |
K100A |
Reduced cleavage of propilin to pilin; when associated with A-97 |
seeded from UniProt:P04737 | ||||
|
traAF101A |
F101A |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer |
seeded from UniProt:P04737 | ||||
|
traAF105V |
F105V |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer |
seeded from UniProt:P04737 | ||||
|
traAF111V |
F111V |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer |
seeded from UniProt:P04737 | ||||
|
traAG120D |
G120D |
Resistant to phages R17 and QB attachment |
seeded from UniProt:P04737 | ||||
|
traAD58A |
D58A |
No production of propilin or pilin |
seeded from UniProt:P04737 | ||||
|
traAG9S |
G9S |
No production of pili |
seeded from UniProt:P04737 | ||||
|
traAP13S |
P13S |
No production of pili |
seeded from UniProt:P04737 | ||||
|
traAK68A |
K68A |
No production of propilin or pilin |
seeded from UniProt:P04737 | ||||
|
traAF71V |
F71V |
No effect on propilin synthesis. Defective pilus tip assembly |
seeded from UniProt:P04737 | ||||
|
traAK79A |
K79A |
No effect on propilin synthesis. Resistant to phage R17 attachment |
seeded from UniProt:P04737 | ||||
|
traAD74G |
D74G |
No effect on propilin synthesis. No effect on pilus formation. Resistant to phage R17 attachment |
seeded from UniProt:P04737 | ||||
|
traAL121S |
L121S |
No effect on propilin synthesis. Defective pilus tip assembly |
seeded from UniProt:P04737 | ||||
|
traAA69E |
A69E |
No effect on propilin synthesis. No effect on pilus formation. Resistant to phage R17 attachment |
seeded from UniProt:P04737 | ||||
|
traAK68E |
K68E |
Resistant to phage R17 attachment |
seeded from UniProt:P04737 | ||||
|
traAG72D |
G72D |
No effect on propilin synthesis. No effect on pilus formation |
seeded from UniProt:P04737 | ||||
|
traAK73A,T |
K73A,T |
No effect on propilin synthesis. No effect on pilus formation |
seeded from UniProt:P04737 | ||||
|
traAK79I |
K79I |
No effect on propilin synthesis. Defective pilus tip assembly |
seeded from UniProt:P04737 | ||||
|
traAV81I |
V81I |
Slightly reduced phage R17 attachment, reduced ability to retract |
seeded from UniProt:P04737 | ||||
|
traAE85A,G |
E85A,G |
No effect on propilin synthesis. Defective pilus tip assembly |
seeded from UniProt:P04737 | ||||
|
traAY93D |
Y93D |
Production of small amounts of propilin and pilin |
seeded from UniProt:P04737 | ||||
|
traAK97A,R,V |
K97A,R,V |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse |
seeded from UniProt:P04737 | ||||
|
traAK97A |
K97A |
Reduced cleavage of propilin to pilin; when associated with A-100 |
seeded from UniProt:P04737 | ||||
|
traAN98A |
N98A |
No effect on propilin synthesis. No effect on pilus formation |
seeded from UniProt:P04737 | ||||
|
traAV99A |
V99A |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse |
seeded from UniProt:P04737 | ||||
|
traAK100A,R,T |
K100A,R,T |
No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer |
seeded from UniProt:P04737 | ||||
| edit table |
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki. <protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
| edit table |
See Help:Gene_resources for help entering information into the Genetic Resources table. <protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
See Help:Gene_accessions for help with entering information into the Gene Accessions table. <protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
traA |
|---|---|
| Synonyms |
traA, Fpla076 |
| Product description |
traA this pilus subunit is processed to propilin and then N-acetylated by TraX; the subunits lying in the inner membrane are assembled to form the pilus, a long extracellular filament that is essential for transfer, contacts the recipient cell and retracts, bring the cells into close contact prior to conjugal DNA transfer |
| EC number (for enzymes) |
|
| edit table |
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Function
<protect>
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
GO:0009418 |
pilus shaft |
PMID:11849547 |
IMP: Inferred from Mutant Phenotype |
C |
complete | |||
|
GO:0044096 |
type IV pilus |
PMID:11849547 |
IMP: Inferred from Mutant Phenotype |
C |
complete | |||
|
GO:0009291 |
unidirectional conjugation |
PMID:4555413 |
IGI: Inferred from Genetic Interaction |
EcoliWiki:traA
|
P |
complete | ||
|
GO:0019062 |
virion attachment to host cell surface receptor |
PMID:11849547 |
IMP: Inferred from Mutant Phenotype |
P |
complete | |||
|
GO:0044096 |
type IV pilus |
PMID:8444800 |
IDA: Inferred from Direct Assay |
C |
complete | |||
| edit table |
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
| edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
See Help:Product_localization for how to add or edit information in this section of EcoliWiki. <protect></protect>
Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MNAVLSVQGA SAPVKKKSFF SKFTRLNMLR LARAVIPAAV LMMFFPQLAM AAGSSGQDLM ASGNTTVKAT FGKDSSVVKW VVLAEVLVGA VMYMMTKNVK FLAGFAIISV FIAVGMAVVG L |
| Length |
121 |
| Mol. Wt |
12.767 kDa |
| pI |
11.0 (calculated) |
| Extinction coefficient |
6,990 (calc based on 1 Y, 1 W, and 0 C residues) |
| edit table |
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki. | </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
See Help:Product_accessions for help entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
| edit table |
Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki. </protect>
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_002483:68245..68285
source=plasmid_F
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
</protect>
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
| edit table |
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
| edit table |
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Accessions Related to Plasmid F traA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
See Help:Expression_accessions for help entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
Evolution
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Homologs in Other Organisms
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| Organism | Homologs (Statistics) | Comments |
|---|---|---|
| edit table |
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Do-It-Yourself Web Tools
<protect></protect>
Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
See Help:Evolution_families for help entering or editing information in this section of EcoliWiki. <protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
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