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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Quickview

Standard Name	II
Gene Synonym(s)	X
Product Desc.	II: site-specific endonuclease-ligase ^[1] ^[2]
Product Synonyms(s)	II, protein II ^[3], Rep ^[4], G2P ^[4], Gene 2 protein ^[4] protein X, G10P ^[4], Gene 10 protein
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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References

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See Help:References for how to manage references in EcoliWiki.

↑ Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9
↑ Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed
↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
↑ ^4.0 ^4.1 ^4.2 ^4.3 UniProt:P69546 (REP_BPF1) http://www.uniprot.org/uniprot/P69546 Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content

Gene

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Gene

Location(s) and DNA Sequence

Sequence Features

Alleles and Phenotypes

Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	II
Mnemonic
Synonyms	X
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Notes

Location(s) and DNA Sequence

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<protect> <protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
		J02448: 5204..7209 <gbrowseImage> name=J02448:5204..7209 source=bacteriophage_f1 preset=GeneLocation </gbrowseImage>	GBrowse (Phage_f1)	FASTA Dump
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Notes

Sequence Features

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<protect> See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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<protect> See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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<protect> See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Product(s)

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Gene Product(s)

Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Replication-associated protein G2P ^[1]
Synonyms	II, protein II ^[2], Rep ^[1], G2P ^[1], Gene 2 protein ^[1] protein X, G10P ^[1], Gene 10 protein
Product description	II: site-specific endonuclease-ligase ^[3] ^[4]
EC number (for enzymes)	3.1.21.- 6.5.1.1
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Notes

The region of DNA that encodes protein II also encodes protein X; an in-frame start at codon 300 produces the 13kD protein X while the full length protein II is 46kD. ^[5]

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Notes	Status
	GO:0045070	positive regulation of viral genome replication	PMID:3054123	IDA: Inferred from Direct Assay	P	pII	complete
	GO:0045071	negative regulation of viral genome replication	PMID:3054123	IDA: Inferred from Direct Assay	P	pX	complete
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

<protect> See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MIDMLVLRLP FIDSLVCSRL SGNDLIAFVD LSKIATLSGM NLSARTVEYH IDGDLTVSGL SHPFESLPTH YSGIAFKIYE GSKNFYPCVE IKASPAKVLQ GHNVFGTTDL ALCSEALLLN FANSLPCLYD LLDVNATTIS RIDATFSARA PNENIAKQVI DHLRNVSNGQ TKSTRSQNWE STVTWNETSR HRTLVAYLKH VELQHQIQQL SSKPSAKMTS YQKEQLKVLS NPDLLEFASG LVRFEARIKT RYLKSFGLPL NLFDAIRFAS DYNSQGKDLI FDLWSFSFSE LFKAFEGDSM NIYDDSAVLD AIQSKHFTIT PSGKTSFAKA SRYFGFYRRL VNEGYDSVAL TMPRNSFWRY VSALVECGIP KSQLMNLSTC NNVVPLVRFI NVDFSSQRPD WYNEPVLKIA
Length	410
Mol. Wt	46.167 kDa
pI	7.8 (calculated)
Extinction coefficient	49,850 - 50,600 (calc based on 15 Y, 5 W, and 6 C residues)
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Domains/Motifs/Modification Sites

<protect> See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_II taxon=10863 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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<protect> See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 UniProt:P69546 (REP_BPF1) http://www.uniprot.org/uniprot/P69546 Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content
↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
↑ Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9
↑ Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed
↑ Yen, TS & Webster, RE (1982) Translational control of bacteriophage f1 gene II and gene X proteins by gene V protein. Cell 29 337-45 PubMed

Expression

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Expression

Overview

Intracellular Levels

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<protect> See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Notes

Translation and Regulation of Translation

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<gbrowseImage> name=J02448:5986..6026 source=bacteriophage_f1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

Turnover and Regulation of Turnover

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Notes

Experimental

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<protect> Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies <protect> See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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Expression Resources <protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to Phage_f1_II Expression

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

Evolution

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Evolution

Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

Phage f1 II:On One Page

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Notes

References

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

Expression

Overview

Intracellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to Phage_f1_II Expression

Notes

Links

References

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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