Phage f1 II:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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References Suggestions
Standard Name

II

Gene Synonym(s)

X

Product Desc.

II: site-specific endonuclease-ligase [1] [2]

Product Synonyms(s)

II, protein II [3], Rep [4], G2P [4], Gene 2 protein [4]

protein X, G10P [4], Gene 10 protein

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
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Notes

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References

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  1. ↑ Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9
  2. ↑ Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed
  3. ↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  4. ↑ 4.0 4.1 4.2 4.3 UniProt:P69546 (REP_BPF1) http://www.uniprot.org/uniprot/P69546

Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

II

Mnemonic
Synonyms

X

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

J02448: 5204..7209
<gbrowseImage> name=J02448:5204..7209 source=bacteriophage_f1 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments
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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference
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Notes

Accessions in Other Databases

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Database Accession Notes
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Notes

Links

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Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Replication-associated protein G2P [1]

Synonyms

II, protein II [2], Rep [1], G2P [1], Gene 2 protein [1]

protein X, G10P [1], Gene 10 protein

Product description

II: site-specific endonuclease-ligase [3] [4]

EC number (for enzymes)

3.1.21.-

6.5.1.1

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Notes

The region of DNA that encodes protein II also encodes protein X; an in-frame start at codon 300 produces the 13kD protein X while the full length protein II is 46kD. [5]

Function

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Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0045070

positive regulation of viral genome replication

PMID:3054123[6]

IDA: Inferred from Direct Assay

P

pII

complete

GO:0045071

negative regulation of viral genome replication

PMID:3054123[6]

IDA: Inferred from Direct Assay

P

pX

complete

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Interactions

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Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MIDMLVLRLP FIDSLVCSRL SGNDLIAFVD LSKIATLSGM NLSARTVEYH IDGDLTVSGL
SHPFESLPTH YSGIAFKIYE GSKNFYPCVE IKASPAKVLQ GHNVFGTTDL ALCSEALLLN
FANSLPCLYD LLDVNATTIS RIDATFSARA PNENIAKQVI DHLRNVSNGQ TKSTRSQNWE
STVTWNETSR HRTLVAYLKH VELQHQIQQL SSKPSAKMTS YQKEQLKVLS NPDLLEFASG
LVRFEARIKT RYLKSFGLPL NLFDAIRFAS DYNSQGKDLI FDLWSFSFSE LFKAFEGDSM
NIYDDSAVLD AIQSKHFTIT PSGKTSFAKA SRYFGFYRRL VNEGYDSVAL TMPRNSFWRY
VSALVECGIP KSQLMNLSTC NNVVPLVRFI NVDFSSQRPD WYNEPVLKIA
Length

410

Mol. Wt

46.167 kDa

pI

7.8 (calculated)

Extinction coefficient

49,850 - 50,600 (calc based on 15 Y, 5 W, and 6 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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<motif_map/>

Structure

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Structures

<beststructure> gene=Phage_f1_II taxon=10863 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources


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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links



References


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 UniProt:P69546 (REP_BPF1) http://www.uniprot.org/uniprot/P69546
  2. ↑ NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  3. ↑ Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9
  4. ↑ Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed
  5. ↑ Yen, TS & Webster, RE (1982) Translational control of bacteriophage f1 gene II and gene X proteins by gene V protein. Cell 29 337-45 PubMed
  6. ↑ 6.0 6.1 Fulford, W & Model, P (1988) Regulation of bacteriophage f1 DNA replication. I. New functions for genes II and X. J. Mol. Biol. 203 49-62 PubMed

Categories


Expression


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Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

Intracellular Levels


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Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)
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Notes

Transcription and Transcriptional Regulation


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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|} </protect>

Notes

Translation and Regulation of Translation


<gbrowseImage> name=J02448:5986..6026 source=bacteriophage_f1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

Turnover and Regulation of Turnover

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Notes

Experimental


Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies

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Type Reference Notes
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Expression Resources

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Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to Phage_f1_II Expression


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Database Accession Notes
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Notes

Links



References


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Evolution


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Homologs Families Links References Suggestions

Homologs in Other Organisms


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Organism Homologs (Statistics) Comments
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Do-It-Yourself Web Tools


Notes

Families


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Database Accession Notes
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Notes

Links



References


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Categories


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