Phage f1 II:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

Replication-associated protein G2P [1]

Synonyms

II, protein II [2], Rep [1], G2P [1], Gene 2 protein [1]

protein X, G10P [1], Gene 10 protein

Product description

II: site-specific endonuclease-ligase [3] [4]

EC number (for enzymes)

3.1.21.-

6.5.1.1

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Notes

The region of DNA that encodes protein II also encodes protein X; an in-frame start at codon 300 produces the 13kD protein X while the full length protein II is 46kD. [5]

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0045070

positive regulation of viral genome replication

PMID:3054123[6]

IDA: Inferred from Direct Assay

P

pII

complete

GO:0045071

negative regulation of viral genome replication

PMID:3054123[6]

IDA: Inferred from Direct Assay

P

pX

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

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Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence
MIDMLVLRLP FIDSLVCSRL SGNDLIAFVD LSKIATLSGM NLSARTVEYH IDGDLTVSGL
SHPFESLPTH YSGIAFKIYE GSKNFYPCVE IKASPAKVLQ GHNVFGTTDL ALCSEALLLN
FANSLPCLYD LLDVNATTIS RIDATFSARA PNENIAKQVI DHLRNVSNGQ TKSTRSQNWE
STVTWNETSR HRTLVAYLKH VELQHQIQQL SSKPSAKMTS YQKEQLKVLS NPDLLEFASG
LVRFEARIKT RYLKSFGLPL NLFDAIRFAS DYNSQGKDLI FDLWSFSFSE LFKAFEGDSM
NIYDDSAVLD AIQSKHFTIT PSGKTSFAKA SRYFGFYRRL VNEGYDSVAL TMPRNSFWRY
VSALVECGIP KSQLMNLSTC NNVVPLVRFI NVDFSSQRPD WYNEPVLKIA
Length

410

Mol. Wt

46.167 kDa

pI

7.8 (calculated)

Extinction coefficient

49,850 - 50,600 (calc based on 15 Y, 5 W, and 6 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References

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Structure <protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_f1_II taxon=10863 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 UniProt:P69546 (REP_BPF1) http://www.uniprot.org/uniprot/P69546 Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content Cite error: Invalid <ref> tag; name "uniprot:P69546" defined multiple times with different content
  2. NCBI: Genome of bacteriophage f1 (closely related to phages fd and m13) http://www.ncbi.nlm.nih.gov/nuccore/V00606.1
  3. Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9
  4. Model, P et al. (1982) The replication of bacteriophage f1: gene V protein regulates the synthesis of gene II protein. Cell 29 329-35 PubMed
  5. Yen, TS & Webster, RE (1982) Translational control of bacteriophage f1 gene II and gene X proteins by gene V protein. Cell 29 337-45 PubMed
  6. 6.0 6.1 Fulford, W & Model, P (1988) Regulation of bacteriophage f1 DNA replication. I. New functions for genes II and X. J. Mol. Biol. 203 49-62 PubMed

Categories

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