PMID:3054123
| Citation |
Fulford, W and Model, P (1988) Regulation of bacteriophage f1 DNA replication. I. New functions for genes II and X. J. Mol. Biol. 203:49-62 |
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| Abstract |
Gene II protein is required for all phases of filamentous phage DNA synthesis other than the conversion of the infecting single strand to the parental double-stranded molecule. It introduces a specific nick into the double-stranded replicative form DNA, is required for the initiation of (+) strand synthesis and is responsible for termination and ring closure of the (+) strand product. Here we show that the gene II protein also promotes minus strand synthesis later in infection. Over-expression of gene II protein can induce the conversion of all nascent single-stranded phage DNA to the double-stranded form, even in the presence of the single-stranded DNA-binding gene V protein that would normally sequester the newly synthesized single strands. We also present evidence that the gene X protein (separately translated from an initiator codon within gene II, and identical to the C-terminal one-third of the gene II protein) is a powerful inhibitor of phage-specific DNA synthesis in vivo. |
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| Keywords |
Base Sequence; Coliphages/genetics; DNA Replication; DNA, Viral/biosynthesis; Escherichia coli; Genes, Viral; Isopropyl Thiogalactoside; Molecular Sequence Data; Plasmids; Protein Biosynthesis; Viral Proteins/genetics; Virus Replication |
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