hscA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hscA

Mnemonic

Heat shock cognate

Synonyms

ECK2523, b2526, JW2510, hsc, hsc66[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.23 minutes 

MG1655: 2656957..2655107
gbrowse_img.php?coord=2655107..2656957&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2579634..2577784
gbrowse_img.php?coord=2577784..2579634&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2540912..2542762
gbrowse_img.php?coord=2540912..2542762&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2657591..2655741
gbrowse_img.php?coord=2655741..2657591&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2748722..2746872
gbrowse_img.php?coord=2746872..2748722&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhscA (Keio:JW2510)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9998[4]

hscAS216F

S216F

(in hsca1)

Strain variation; seeded from UniProt:P0A6Z1

ΔhscA772::kan

PMID:16738554[3]

CGSC:101408



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2510

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCCTTATTACAAATTAGTGA

Primer 2:CCAACCTCGTCCACGGAATGGC

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: 4% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[4]

est. P1 cotransduction: 76% [6]
Synonyms:zff-208::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12130

EchoBASE (EcoliWiki Page)

EchoBASE:EB2051

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2526

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3051

EcoGene (EcoliWiki Page)

EcoGene:EG12130

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HSCA

CGSC (EcoliWiki Page)

CGSC:32977

ASAP (EcoliWikiPage)

ASAP:ABE-0008315



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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