ftsK:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ftsK |
|---|---|
| Mnemonic |
filamentation temperature sensitive |
| Synonyms |
ECK0881, b0890, JW0873, dinH[1] |
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Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
20.1 minutes |
MG1655: 932447..936436 |
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NC_012967: 950282..954310 |
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|
W3110 |
|
W3110: 933646..937635 |
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DH10B: 986375..990364 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔLC |
Carries a deletion of FtsK L and FtsK C | ||||||
|
NC |
Barre |
Deletion of the FtsK Linker | |||||
|
NL hi C |
Barre |
Carries swaps b/t E.coli and H. influenzae FtsK regions | |||||
|
ftsK A |
K997A |
Barre |
Carries a single point mutation in the walker A motif of FtsK C | ||||
|
NLC hi |
Barre |
Carries swaps b/t E.coli and H.influenzae FtsK regions | |||||
|
ftsk::cat1 |
Donachie Sheratt |
only expresses the NTD due to a Cm resistance cassette at nt 678 of ftsK | |||||
|
ftsk44 |
Growth Phenotype |
temperature sensitive late stage block in division, but does not affect chromosome replication or segregation |
Donachie |
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|
ftsK1::cat |
Other |
chromosome segregation |
defect in the C-terminus of FtsK | ||||
|
ftsK1::cat ΔmukB |
Growth Phenotype |
synthetically lethal |
shown that it must be complemented in trans | ||||
|
ftsK::cat-2 |
Δ54-2201 |
almost the entire N-terminal membrane-spanning domain is deleted, and the C-terminal fragment is out of frame with the cat gene. Lethal- must be complemented in trans | |||||
|
ftsK::cat-Δ5 |
Δ54-3651 |
this is a deletion of 90% of ftsK | |||||
|
ftsK3531 |
missense mutation |
Growth Phenotype |
same phenotype as ftsK44- temperature sensitive late stage block in division, but does not affect chromosome replication or segregation |
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|
ftsK::cat-Δ2 ΔdacA::kan |
Cell Shape |
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ftsK::cat-Δ5 Δdac::kan |
Other |
synthetically lethal |
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|
ftsKE58A |
E58A |
Loss of function |
seeded from UniProt:P46889 | ||||
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Notes
Deletions that leave the membrane segments intact are viable.[7]
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0873 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGCCAGGAATACATTGAAGA Primer 2:CCGTCAAACGGCGGTGGGGCCAG | |
|
zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: % [10] | |
|
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 61% [10] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 Bigot, S et al. (2004) FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol. Microbiol. 54 876-86 PubMed
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
- ↑ Begg, KJ et al. (1995) A new Escherichia coli cell division gene, ftsK. J. Bacteriol. 177 6211-22 PubMed
- ↑ Diez, AA et al. (1997) A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA. J. Bacteriol. 179 5878-83 PubMed
- ↑ 6.0 6.1 Yu, XC et al. (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol. 180 6424-8 PubMed
- ↑ Draper, GC et al. (1998) Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180 4621-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).