mutL:Gene Product(s): Difference between revisions

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Added to the category for various RefGenome genes.
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418591 RefSeq:NP_418591]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418591 RefSeq:NP_418591]
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[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
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[http://salilab.org/modbase/searchbyid?databaseID=P23367 ModBase:P23367]
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[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
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[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1B63 PDB:1B63]
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Revision as of 16:27, 8 October 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MutL

Synonyms

methyl-directed mismatch repair protein product[1], B4170 product[2][1], MutL product[2][1]

Product description

MutL[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair; matchmaker protein; weak DNA-stimulated ATPase[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0006298

mismatch repair

PMID:6987663[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0005524

ATP binding

PMID:9827806[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0006298

mismatch repair

PMID:2665076[8]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0016887

ATPase activity

PMID:9827806[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0016887

ATPase activity

PMID:9827806[7]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0032300

mismatch repair complex

PMID:6987663[5]

IGI: Inferred from Genetic Interaction

UniProtKB:P06722|UniProtKB:P23909

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc [9]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002099
InterPro:IPR003594
InterPro:IPR013507
SP_KW:KW-0067

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0006281

DNA repair

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc [9]

complete

GO:0006298

mismatch repair

GO_REF:0000002
GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002099
InterPro:IPR013507
HAMAP:MF_00149

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006974

response to DNA damage stimulus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc [9]

complete

GO:0030983

mismatched DNA binding

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002099
InterPro:IPR013507

F

Seeded from EcoCyc [9]

complete

GO:0005515

protein binding

PMID:15690043[10]
PMID:9482750[11]
PMID:9880500[12]

IPI: Inferred from Physical Interaction

UniProtKB:P0A6N1
UniProtKB:P03018
UniProtKB:P06722

F

Seeded from EcoCyc [9]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006298

mismatch repair

PMID:6987663[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005524

ATP binding

PMID:9827806[7]

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

PMID:2665076[8]

IDA: Inferred from Direct Assay

P

complete

GO:0032300

mismatch repair complex

PMID:6987663[5]

IGI: Inferred from Genetic Interaction

UniProtKB:P06722|UniProtKB:P23909

C

With MutH (P06722) and MutS (P23909).

complete

GO:0016887

ATPase activity

PMID:9827806[7]

IDA: Inferred from Direct Assay

F

complete

GO:0016887

ATPase activity

PMID:9827806[7]

IMP: Inferred from Mutant Phenotype

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of MutHLS complex, methyl-directed mismatch repair

PMID:6987663[5]

Inferred from genetic interaction

Protein

tufA

PMID:15690043[10]

Experiment(s):EBI-880976

Protein

dnaK

PMID:15690043[10]

Experiment(s):EBI-887579

Protein

rpsA

PMID:15690043[10]

Experiment(s):EBI-887579

Protein

Vsr

PMID:19376855[13]

Protein

DnaN: beta sliding clamp of DNA Pol III

MutL only binds to the beta subunit in the presence of ss DNA.

PMID:16546997[14]

native gel electrophoresis

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MPIQVLPPQL ANQIAAGEVV ERPASVVKEL VENSLDAGAT RIDIDIERGG AKLIRIRDNG
CGIKKDELAL ALARHATSKI ASLDDLEAII SLGFRGEALA SISSVSRLTL TSRTAEQQEA
WQAYAEGRDM NVTVKPAAHP VGTTLEVLDL FYNTPARRKF LRTEKTEFNH IDEIIRRIAL
ARFDVTINLS HNGKIVRQYR AVPEGGQKER RLGAICGTAF LEQALAIEWQ HGDLTLRGWV
ADPNHTTPAL AEIQYCYVNG RMMRDRLINH AIRQACEDKL GADQQPAFVL YLEIDPHQVD
VNVHPAKHEV RFHQSRLVHD FIYQGVLSVL QQQLETPLPL DDEPQPAPRS IPENRVAAGR
NHFAEPAARE PVAPRYTPAP ASGSRPAAPW PNAQPGYQKQ QGEVYRQLLQ TPAPMQKLKA
PEPQEPALAA NSQSFGRVLT IVHSDCALLE RDGNISLLSL PVAERWLRQA QLTPGEAPVC
AQPLLIPLRL KVSAEEKSAL EKAQSALAEL GIDFQSDAQH VTIRAVPLPL RQQNLQILIP
ELIGYLAKQS VFEPGNIAQW IARNLMSEHA QWSMAQAITL LADVERLCPQ LVKTPPGGLL
QSVDLHPAIK ALKDE
Length

615

Mol. Wt

67.924 kDa

pI

6.8 (calculated)

Extinction coefficient

54,890 - 55,765 (calc based on 11 Y, 7 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mutL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11281-MONOMER

UniProt (EcoliWiki Page)

UniProt:P23367

PFAM (EcoliWiki Page)

PFAM:PF02518

RefSeq (EcoliWiki Page)

RefSeq:NP_418591


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Glickman, BW & Radman, M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 77 1063-7 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Ban, C & Yang, W (1998) Crystal structure and ATPase activity of MutL: implications for DNA repair and mutagenesis. Cell 95 541-52 PubMed
  8. ↑ 8.0 8.1 Lahue, RS et al. (1989) DNA mismatch correction in a defined system. Science 245 160-4 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  10. ↑ 10.0 10.1 10.2 10.3 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  11. ↑ Hall, MC et al. (1998) Evidence for a physical interaction between the Escherichia coli methyl-directed mismatch repair proteins MutL and UvrD. EMBO J. 17 1535-41 PubMed
  12. ↑ Hall, MC & Matson, SW (1999) The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity. J. Biol. Chem. 274 1306-12 PubMed
  13. ↑ Polosina, YY et al. (2009) The Escherichia coli mismatch repair protein MutL recruits the Vsr and MutH endonucleases in response to DNA damage. J. Bacteriol. 191 4041-3 PubMed
  14. ↑ López de Saro, FJ et al. (2006) The beta sliding clamp binds to multiple sites within MutL and MutS. J. Biol. Chem. 281 14340-9 PubMed

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