mutL:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MutL |
|---|---|
| Synonyms |
methyl-directed mismatch repair protein[1], B4170[2][1], MutL[2][1] , ECK4166, JW4128, b4170 |
| Product description |
Component of MutHLS complex, methyl-directed mismatch repair[2][3] Methyl-directed mismatch repair; matchmaker protein; weak DNA-stimulated ATPase[4] |
| EC number (for enzymes) |
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| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0032300 |
mismatch repair complex |
IGI: Inferred from Genetic Interaction |
C |
With MutH (P06722) and MutS (P23909). |
complete | |||
| GO:0005515 |
protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016887 |
ATPase activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006298 |
mismatch repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030983 |
mismatched DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003677 |
DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0000018 |
regulation of DNA recombination |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of MutHLS complex, methyl-directed mismatch repair |
Inferred from genetic interaction | ||
|
Protein |
tufA |
Experiment(s):EBI-880976 | ||
|
Protein |
dnaK |
Experiment(s):EBI-887579 | ||
|
Protein |
rpsA |
Experiment(s):EBI-887579 | ||
|
Protein |
rpsA |
LCMS(ID Probability):99.6 | ||
|
Protein |
Vsr |
|||
|
Protein |
DnaN: beta sliding clamp of DNA Pol III |
MutL only binds to the beta subunit in the presence of ss DNA. |
native gel electrophoresis | |
|
Protein |
MutH |
data suggest the ATPase fragment of MutL directly contacts MutH |
Chemical crosslinking | |
|
Protein |
MutS |
Visualized using EM | ||
|
Protein |
UvrD |
2-Hybrid Screen | ||
|
Protein |
MutH |
The C-terminal 218 amino acids of MutL were sufficient for the two-hybrid interaction with MutH |
2 Hybrid Screen | |
|
Protein |
UvrD |
Both the N- and C-termini of UvrD were required for interaction with MutL |
2 Hybrid Screen | |
|
Protein |
The N-terminal fragment of MutL (residues 1-349) was sufficient to bind RecA. MutL inhibits the ssDNA-dependent ATPase activity of RecA (Fig. 4) |
Far-Western and pull-down assays. | ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MPIQVLPPQL ANQIAAGEVV ERPASVVKEL VENSLDAGAT RIDIDIERGG AKLIRIRDNG CGIKKDELAL ALARHATSKI ASLDDLEAII SLGFRGEALA SISSVSRLTL TSRTAEQQEA WQAYAEGRDM NVTVKPAAHP VGTTLEVLDL FYNTPARRKF LRTEKTEFNH IDEIIRRIAL ARFDVTINLS HNGKIVRQYR AVPEGGQKER RLGAICGTAF LEQALAIEWQ HGDLTLRGWV ADPNHTTPAL AEIQYCYVNG RMMRDRLINH AIRQACEDKL GADQQPAFVL YLEIDPHQVD VNVHPAKHEV RFHQSRLVHD FIYQGVLSVL QQQLETPLPL DDEPQPAPRS IPENRVAAGR NHFAEPAARE PVAPRYTPAP ASGSRPAAPW PNAQPGYQKQ QGEVYRQLLQ TPAPMQKLKA PEPQEPALAA NSQSFGRVLT IVHSDCALLE RDGNISLLSL PVAERWLRQA QLTPGEAPVC AQPLLIPLRL KVSAEEKSAL EKAQSALAEL GIDFQSDAQH VTIRAVPLPL RQQNLQILIP ELIGYLAKQS VFEPGNIAQW IARNLMSEHA QWSMAQAITL LADVERLCPQ LVKTPPGGLL QSVDLHPAIK ALKDE |
| Length |
615 |
| Mol. Wt |
67.924 kDa |
| pI |
6.8 (calculated) |
| Extinction coefficient |
54,890 - 55,765 (calc based on 11 Y, 7 W, and 7 C residues) |
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Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
|
Purified protein |
MutL R266E[8] |
|
|
Purified protein |
MutL R95F[8] |
|
|
Purified protein |
MutL N302A[8] |
|
|
Purified protein |
MutL R261H[8] |
|
|
Purified protein |
MutL K307A[8] |
|
|
Purified protein |
MutL E29A[8] |
|
|
Purified protein |
MutL R95F/N302A[8] |
|
|
Purified protein |
MutL I90R[8] |
|
|
Purified protein |
MutL I90E[8] |
|
|
Purified protein |
MutL R237E[8] |
|
|
Purified protein |
MutL G238A[8] |
|
|
Purified protein |
MutL K159E[8] |
|
|
Purified protein |
MutL G238D[8] |
|
|
Purified protein |
MutL R177E[8] |
|
|
Purification protocol |
Single-step purification of His6-MutL |
|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 Glickman, BW & Radman, M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc. Natl. Acad. Sci. U.S.A. 77 1063-7 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Ban, C & Yang, W (1998) Crystal structure and ATPase activity of MutL: implications for DNA repair and mutagenesis. Cell 95 541-52 PubMed
- ↑ Lahue, RS et al. (1989) DNA mismatch correction in a defined system. Science 245 160-4 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 Junop, MS et al. (2003) In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination. DNA Repair (Amst.) 2 387-405 PubMed
- ↑ Rayssiguier, C et al. (1989) The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342 396-401 PubMed
- ↑ 10.0 10.1 10.2 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Polosina, YY et al. (2009) The Escherichia coli mismatch repair protein MutL recruits the Vsr and MutH endonucleases in response to DNA damage. J. Bacteriol. 191 4041-3 PubMed
- ↑ López de Saro, FJ et al. (2006) The beta sliding clamp binds to multiple sites within MutL and MutS. J. Biol. Chem. 281 14340-9 PubMed
- ↑ Allen, DJ et al. (1997) MutS mediates heteroduplex loop formation by a translocation mechanism. EMBO J. 16 4467-76 PubMed
- ↑ 15.0 15.1 Hall, MC & Matson, SW (1999) The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity. J. Biol. Chem. 274 1306-12 PubMed
- ↑ Hall, MC et al. (1998) Evidence for a physical interaction between the Escherichia coli methyl-directed mismatch repair proteins MutL and UvrD. EMBO J. 17 1535-41 PubMed
- ↑ Zhang, M et al. (2012) MutL associates with Escherichia coli RecA and inhibits its ATPase activity. Arch. Biochem. Biophys. 517 98-103 PubMed
- ↑ 18.0 18.1 18.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
- ↑ Feng, G & Winkler, ME (1995) Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12. BioTechniques 19 956-65 PubMed
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