adhE:Gene: Difference between revisions

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!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
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''adhE''  
''adhE''  
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!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK1235, b1241, JW1228, adhC, ana''<ref name='LIB:Riley_2006'/>
''ECK1235, b1241, JW1228, adhC, ana''<ref name='LIB:Riley_2006'/>
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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{{GBrowseFastaLink|ecoli|NC_000913|1294669|1297344|FASTA Dump}}
{{GBrowseFastaLink|ecoli|NC_000913|1294669|1297344|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10031 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10031 EcoCyc Sequence Dump]  
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W3110
W3110
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|1297023|1299698|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|AC_000091|1297023|1299698|FASTA Dump}}  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107394 CGSC9113]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107394 CGSC9113]<ref name='LIB:cgsc'/>  
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|[[PMID:6986356]]<ref name=PMID:6986356/>
[[PMID:6986356]]<ref name=PMID:6986356/>
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[[cgscmutation:7028|CGSC:7028]]
[[cgscmutation:7028|CGSC:7028]]
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|[[PMID:6752127]]<ref name=PMID:6752127/>
[[PMID:6752127]]<ref name=PMID:6752127/>
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[[cgscmutation:7285|CGSC:7285]]
[[cgscmutation:7285|CGSC:7285]]
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|[[PMID:6752127]]<ref name=PMID:6752127/>
[[PMID:6752127]]<ref name=PMID:6752127/>
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[[cgscmutation:7457|CGSC:7457]]
[[cgscmutation:7457|CGSC:7457]]
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|[[PMID:3550385]]<ref name=PMID:3550385/>
[[PMID:3550385]]<ref name=PMID:3550385/>
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[[cgscmutation:92529|CGSC:92529]]
[[cgscmutation:92529|CGSC:92529]]
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|[[PMID:3550385]]<ref name=PMID:3550385/>
[[PMID:3550385]]<ref name=PMID:3550385/>
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[[cgscmutation:92530|CGSC:92530]]
[[cgscmutation:92530|CGSC:92530]]
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[[cgscmutation:99914|CGSC:99914]]
[[cgscmutation:99914|CGSC:99914]]
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JW1228
JW1228
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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|[[PMID:16769691]]<ref name=PMID:16769691/>
[[PMID:16769691]]<ref name=PMID:16769691/>


Status:Clone OK
Status:Clone OK
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Primer 2:CCAGCGGATTTTTTCGCTTTTTT  
Primer 2:CCAGCGGATTTTTTCGCTTTTTT  
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[[fadR:Gene|fadR13::Tn10]]
[[fadR:Gene|fadR13::Tn10]]
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est. P1 cotransduction: 4% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 4% <ref name='LIB:Nichols_linked_Tn'/>  
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[[oppC:Gene|oppC506::Tn10]]
[[oppC:Gene|oppC506::Tn10]]
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CAG12169 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38027 CGSC7369]<ref name='LIB:cgsc'/>
CAG12169 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38027 CGSC7369]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 73% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 73% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zch-506::Tn10, zci-506::Tn10


Synonyms:zch-506::Tn10, zci-506::Tn10
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0004164&formSubmitReturn=1 ASAP:ABE-0004164]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0004164&formSubmitReturn=1 ASAP:ABE-0004164]
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<noinclude><protect>
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==References==
==References==
{{BacktoTop}}
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<references/>
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__NOTOC__
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Revision as of 18:37, 21 January 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

adhE

Mnemonic
Synonyms

ECK1235, b1241, JW1228, adhC, ana[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.9 minutes 

MG1655: 1297344..1294669
gbrowse_img.php?coord=1293669..1298344&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1299698..1297023
gbrowse_img.php?coord=1296023..1300698&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔadhE (Keio:JW1228)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9113[3]

ana-1

CGSC:5096

adhE80

PMID:6986356[4]

CGSC:7028

adhE1

PMID:6752127[5]

CGSC:7285

adhE16(ts)

temperature sensitive

CGSC:7289

adhE2

PMID:6752127[5]

CGSC:7457

ana-2

adhE

CGSC:14693

adhE36

PMID:3550385[6]

CGSC:92529

adhE38

PMID:3550385[6]

CGSC:92530

ΔadhE748::kan

PMID:16738554[2]

CGSC:99914


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1228

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGCTGTTACTAATGTCGCTGA

Primer 2:CCAGCGGATTTTTTCGCTTTTTT

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 4% [8]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[3]

est. P1 cotransduction: 73% [8]nnSynonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10031

EchoBASE (EcoliWiki Page)

EchoBASE:EB0030

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1241

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1705

EcoGene (EcoliWiki Page)

EcoGene:EG10031

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ADHE

CGSC (EcoliWiki Page)

CGSC:1041

ASAP (EcoliWikiPage)

ASAP:ABE-0004164


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Clark, D & Cronan, JE Jr (1980) Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J. Bacteriol. 141 177-83 PubMed
  5. ↑ 5.0 5.1 Lorowitz, W & Clark, D (1982) Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase. J. Bacteriol. 152 935-8 PubMed
  6. ↑ 6.0 6.1 Cunningham, PR & Clark, DP (1986) The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol. Gen. Genet. 205 487-93 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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