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!{{table_heading_style}} |Standard name
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''trg''  
''trg''  
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!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK1415, b1421, JW1417''<ref name='LIB:Riley_2006'/>
''ECK1415, b1421, JW1417''<ref name='LIB:Riley_2006'/>
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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{{GBrowseFastaLink|ecoli|NC_000913|1490494|1492134|FASTA Dump}}
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*[http://biocyc.org/ECOLI/sequence?object=EG11018 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11018 EcoCyc Sequence Dump]  
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W3110
W3110
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|1494184|1495824|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|AC_000091|1494184|1495824|FASTA Dump}}  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107523 CGSC9242]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107523 CGSC9242]<ref name='LIB:cgsc'/>  
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|[[PMID:2540407]]<ref name=PMID:2540407/>
[[PMID:2540407]]<ref name=PMID:2540407/>
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[[cgscmutation:10754|CGSC:10754]]
[[cgscmutation:10754|CGSC:10754]]
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|[[PMID:2540407]]<ref name=PMID:2540407/>
[[PMID:2540407]]<ref name=PMID:2540407/>
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[[cgscmutation:39820|CGSC:39820]]
[[cgscmutation:39820|CGSC:39820]]
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[[cgscmutation:103208|CGSC:103208]]
[[cgscmutation:103208|CGSC:103208]]
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JW1417
JW1417
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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|[[PMID:16769691]]<ref name=PMID:16769691/>
[[PMID:16769691]]<ref name=PMID:16769691/>


Status:Clone OK
Status:Clone OK
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Primer 2:CCaACCGTAGCGAAACTAACTGG  
Primer 2:CCaACCGTAGCGAAACTAACTGG  
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[[ompN:Gene|zhj-3076::Tn10]]
[[ompN:Gene|zhj-3076::Tn10]]
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est. P1 cotransduction: 6% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 6% <ref name='LIB:Nichols_linked_Tn'/>  
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[[yddE:Gene|zdd-235::Tn10]]
[[yddE:Gene|zdd-235::Tn10]]
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CAG18461 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39848 CGSC7378]<ref name='LIB:cgsc'/>
CAG18461 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39848 CGSC7378]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 13% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 13% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zdc-235::Tn10


Synonyms:zdc-235::Tn10
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0004744&formSubmitReturn=1 ASAP:ABE-0004744]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0004744&formSubmitReturn=1 ASAP:ABE-0004744]
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__NOTOC__
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Revision as of 18:35, 21 January 2009

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

trg

Mnemonic
Synonyms

ECK1415, b1421, JW1417[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

32.12 minutes 

MG1655: 1490494..1492134
gbrowse_img.php?coord=1489494..1493134&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1494184..1495824
gbrowse_img.php?coord=1493184..1496824&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δtrg (Keio:JW1417)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9242[3]

trg-2::Tn10

Insertion at 1,491,930 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12026 = CGSC7376[3]

Synonyms:

trg-1::Tn5

CGSC:9685

trg-2::Tn10

PMID:2540407[5]

CGSC:10754

trg-3120::Tn10kan

PMID:2540407[5]

CGSC:39820

Δtrg-782::kan

PMID:16738554[2]

CGSC:103208


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1417

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAATACAACTCCCTCACAGCG

Primer 2:CCaACCGTAGCGAAACTAACTGG

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[3]

est. P1 cotransduction: 6% [4]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[3]

est. P1 cotransduction: 13% [4]nnSynonyms:zdc-235::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11018

EchoBASE (EcoliWiki Page)

EchoBASE:EB1011

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1421

EcoGene (EcoliWiki Page)

EcoGene:EG11018

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TRG

CGSC (EcoliWiki Page)

CGSC:85

ASAP (EcoliWikiPage)

ASAP:ABE-0004744


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ 5.0 5.1 Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed


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