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!Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10837 EcoCyc:EG10837]
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10837 EcoCyc:EG10837]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])  
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])  
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0830 EchoBASE:EB0830]
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0830 EchoBASE:EB0830]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
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[http://chase.ou.edu/macro/searchlink.php?bnum=b3778 EcoliGenExpDB:b3778]
[http://chase.ou.edu/macro/searchlink.php?bnum=b3778 EcoliGenExpDB:b3778]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
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[http://BioCyc.org/ECOL199310/new-image?object=C4698 ECOL199310CYC:C4698]
[http://BioCyc.org/ECOL199310/new-image?object=C4698 ECOL199310CYC:C4698]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
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[http://ecogene.org/geneinfo.php?eg_id=EG10837 EcoGene:EG10837]
[http://ecogene.org/geneinfo.php?eg_id=EG10837 EcoGene:EG10837]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
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[http://BioCyc.org/ECOO157/new-image?object=REP ECOO157CYC:REP]
[http://BioCyc.org/ECOO157/new-image?object=REP ECOO157CYC:REP]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])  
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])  
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/303 CGSC:303]
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/303 CGSC:303]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0012344&formSubmitReturn=1 ASAP:ABE-0012344] 
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Revision as of 00:53, 16 September 2008

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

rep

Mnemonic
Synonyms

ECK3770, b3778, JW5604, dasC, mbrA, mmrA, dasC?[1][2]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.32 minutes 

MG1655: 3958700..3960721
gbrowse_img.php?coord=3957700..3961721&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3676004..3673983
gbrowse_img.php?coord=3672983..3677004&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δrep (Keio:JW5604)

deletion

deletion

PMID:16738554[3]

Shigen

rep-5

CGSC:7013

rep-3

PMID:383240[4]

CGSC:9794

rep-142(ts)

temperature sensitive

CGSC:90737

[rep-71]

PMID:6286604[5]

CGSC:90823

null allele

Δrep-729::kan

PMID:16738554[3]

CGSC:113537


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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW5604

Plasmid clone

Shigen

PMID:16769691[6]

Status:cloned as old JW

Primer 1:GCCCGTCTAAACCCCGGCCAACA

Primer 2:CCTTTCCCTCGTTTTGCCGCCAT

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[7]

est. P1 cotransduction: 83% [8]

Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[7]

est. P1 cotransduction: 10% [8]

Synonyms:metE3079::Tn10


The Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10837

EchoBASE (EcoliWiki Page)

EchoBASE:EB0830

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3778

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4698

EcoGene (EcoliWiki Page)

EcoGene:EG10837

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:REP

CGSC (EcoliWiki Page)

CGSC:303

ASAP (EcoliWikiPage)

ASAP:ABE-0012344


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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Takahashi, S et al. (1979) The rep mutation. VI. Purification and properties of the Escherichia coli rep protein, DNA helicase III. Can. J. Biochem. 57 855-66 PubMed
  5. ↑ Tessman, I et al. (1982) Relative map location of the rep and rho genes of Escherichia coli. J. Bacteriol. 151 1637-40 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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