Latest revision as of 05:20, 29 July 2007

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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	zapA
Gene Synonym(s)	ECK2905, b2910, JW2878, ygfE^[1], ygfE
Product Desc.	protein that localizes to the cytokinetic ring^[2]^[3]; Component of protein that localizes to the cytokinetic ring^[3] FtsZ-associated protein^[4]
Product Synonyms(s)	protein that localizes to the cytokinetic ring^[1], B2910^[2]^[1], ZapA^[2]^[1], YgfE^[2]^[1] , ECK2905, JW2878, ygfE, b2910
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): zapA^[2]
Regulation/Activity
Quick Links
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Notes

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ZapA binding promotes FtsZ polymer bundling and inhibits the GTPase activity of FtsZ (Small, 2007). Overexpression causes filamentous biofilm formation. Divisome, non-essential.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	zapA
Mnemonic	Z-ring-associated protein
Synonyms	ECK2905, b2910, JW2878, ygfE^[1], ygfE
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	65.82 minutes	MG1655: 3053634..3053963 <gbrowseImage> name=NC_000913:3053634..3053963 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2941363..2941692 <gbrowseImage> name=NC_012967:2941363..2941692 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2940782..2941111 <gbrowseImage> name=NC_012759:2940782..2941111 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3054268..3054597 <gbrowseImage> name=NC_007779:3054268..3054597 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3147504..3147833 <gbrowseImage> name=NC_010473:3147504..3147833 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔzapA (Keio:JW2878)	deletion	deletion	PMID:16738554	Shigen CGSC10232^[5]
ΔzapA761::kan			PMID:16738554	CGSC:106100
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2878	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTGCACAACCCGTCGATAT Primer 2:CCTTCAAAGTTTTGGTTAGTTTT
10B4	Kohara Phage	Genobase	PMID:3038334
1A2	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: % ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12878	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12878	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002417	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947404	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2716	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009554	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ZapA
Synonyms	protein that localizes to the cytokinetic ring^[1], B2910^[2]^[1], ZapA^[2]^[1], YgfE^[2]^[1] , ECK2905, JW2878, ygfE, b2910
Product description	protein that localizes to the cytokinetic ring^[2]^[3]; Component of protein that localizes to the cytokinetic ring^[3] FtsZ-associated protein^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000917	barrier septum formation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_02012	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000917	barrier septum formation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0717	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_02012	C	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_02012	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:20497333^[7]	IPI: Inferred from Physical Interaction	EcoliWiki:zapA	F		complete
	GO:0005515	protein binding	PMID:20497333^[7]	IPI: Inferred from Physical Interaction	EcoliWiki:ftsZ	F		complete
	GO:0005515	protein binding	PMID:20497333^[7]	IPI: Inferred from Physical Interaction	EcoliWiki:ftsN	F		complete
	GO:0005515	protein binding	PMID:20497333^[7]	IPI: Inferred from Physical Interaction	EcoliWiki:ftsI	F		complete
	GO:0000921	septin ring assembly	PMID:15630023^[8]	IDA: Inferred from Direct Assay		P		complete
	GO:0032155	cell division site part	PMID:15630023^[8]	IDA: Inferred from Direct Assay		C		complete
	GO:0051301	cell division	PMID:20487275^[9]	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0044457	cell septum part	PMID:20487275^[9]	IMP: Inferred from Mutant Phenotype		C		complete
	GO:0000917	barrier septum formation	PMID:20487275^[9]	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of protein that localizes to the cytokinetic ring	could be indirect
Protein	thyA		PMID:16606699^[10]	Experiment(s):EBI-1144280
Protein	lpxA		PMID:16606699^[10]	Experiment(s):EBI-1144280
Protein	caiD		PMID:16606699^[10]	Experiment(s):EBI-1144280
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSAQPVDIQI FGRSLRVNCP PDQRDALNQA ADDLNQRLQD LKERTRVTNT EQLVFIAALN ISYELAQEKA KTRDYAASME QRIRMLQQTI EQALLEQGRI TEKTNQNFE
Length	109
Mol. Wt	12.593 kDa
pI	5.0 (calculated)
Extinction coefficient	2,980 - 3,105 (calc based on 2 Y, 0 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..91	PF05164 Cell division protein ZapA		PMID:19920124^[11]
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=zapA taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130812	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947404	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009554	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ADS2	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12878	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12878	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947404	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002417	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2716	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	2275	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	738	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2008	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
zapA
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:3053614..3053654 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2910 (EcoliWiki Page)	NCBI GEO profiles for zapA
microarray	GenExpDB:b2910 (EcoliWiki Page)	Summary of data for zapA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to zapA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12878	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2716	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2910
EcoGene	EcoGene:EG12878	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002417	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009554	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YGFE	From SHIGELLACYC
E. coli O157	YGFE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF05164 Cell division protein ZapA
Superfamily (EcoliWiki Page)	SUPERFAMILY:102829
PFAM (EcoliWiki Page)	PFAM:PF05164
EcoCyc	EcoCyc:EG12878	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12878	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002417	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2716	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009554	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 ^7.3 Alexeeva, S et al. (2010) Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET. Mol. Microbiol. 77 384-98 PubMed
↑ ^8.0 ^8.1 Goehring, NW et al. (2005) Premature targeting of a cell division protein to midcell allows dissection of divisome assembly in Escherichia coli. Genes Dev. 19 127-37 PubMed
↑ ^9.0 ^9.1 ^9.2 Galli, E & Gerdes, K (2010) Spatial resolution of two bacterial cell division proteins: ZapA recruits ZapB to the inner face of the Z-ring. Mol. Microbiol. 76 1514-26 PubMed
↑ ^10.0 ^10.1 ^10.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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zapA:On One Page: Difference between revisions

Latest revision as of 05:20, 29 July 2007

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to zapA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

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