atpA:Gene: Difference between revisions

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Primer 2:CCCCAGGATTGGGTTGCTTTGAA  
Primer 2:CCCCAGGATTGGGTTGCTTTGAA  
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zid-501::Tn10
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Linked marker
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CAG18499 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38945 CGSC7458]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 16% <ref name='LIB:Nichols_linked_Tn'/>
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[[rbsA:Gene|rbsD296::Tn10]]
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Linked marker
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CAG18501 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40333 CGSC7460]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 62% <ref name='LIB:Nichols_linked_Tn'/>
Synonyms:zie-296::Tn10
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Revision as of 23:33, 28 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

atpA

Mnemonic
Synonyms

ECK3727, b3734, JW3712, papA, uncA, unc[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.41 minutes 

MG1655: 3917880..3916339
gbrowse_img.php?coord=3915339..3918880&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3716824..3718365
gbrowse_img.php?coord=3715824..3719365&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability

ΔatpA (Keio:JW3712)

deletion

deletion

PMID:16738554[2]

Shigen

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW3712

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCCAACTGAATTCCACCGAAAT

Primer 2:CCCCAGGATTGGGTTGCTTTGAA

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[4]

est. P1 cotransduction: 16% [5]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[4]

est. P1 cotransduction: 62% [5]

Synonyms:zie-296::Tn10

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10098

EchoBASE (EcoliWiki Page)

EchoBASE:EB0096

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3734

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4660

EcoGene (EcoliWiki Page)

EcoGene:EG10098

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ATPA

CGSC (EcoliWiki Page)

CGSC:33

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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).