Phage lambda xis:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
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xis  
xis
|-
|-
!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
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xis, lambdap34  
xis, lambdap34
|-
|-
!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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||
Excisionase  
Excisionase
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|-
!align=left {{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
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!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
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!align=left {{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
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<pre>MYLTLQEWNA RQRRPRSLET VRRWVRECRI FPPPVKDGRE YLFHESAVKV DLNRPVTGGL
<pre>MYLTLQEWNA RQRRPRSLET VRRWVRECRI FPPPVKDGRE YLFHESAVKV DLNRPVTGGL
LKRIRNGKKA KS</pre>  
LKRIRNGKKA KS</pre>
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!align=left {{table heading style}} |Length
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!|Type!!Residues!!Description!!Notes!!References
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Latest revision as of 20:19, 17 February 2015

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

xis

Synonyms

xis, lambdap34

Product description

Excisionase

EC number (for enzymes)


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Notes

Function

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Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019047

provirus integration

PMID:1055366[1]

IDA: Inferred from Direct Assay

P

negatively affects phage integration (Int activity).

complete

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Interactions

<protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Phage lambda Int

PMID:1396573[2]

See Fig 4.

Protein

E. coli FIS protein

PMID:1396573[2]

See Fig 4.

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MYLTLQEWNA RQRRPRSLET VRRWVRECRI FPPPVKDGRE YLFHESAVKV DLNRPVTGGL
LKRIRNGKKA KS
Length

72

Mol. Wt

8.604 kDa

pI

11.6 (calculated)

Extinction coefficient

13,980 - 14,105 (calc based on 2 Y, 2 W, and 1 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_lambda_xis taxon=10710 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

Biological complex of xis interacting with DNA, PDB:1Rh6
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1RH6.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>









Notes

See Crystal structure of the excisionase-DNA complex from bacteriophage lambda[3] or PDB 1RH6.

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Nash, HA (1975) Integrative recombination of bacteriophage lambda DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 72 1072-6 PubMed
  2. ↑ 2.0 2.1 Numrych, TE et al. (1992) Characterization of the bacteriophage lambda excisionase (Xis) protein: the C-terminus is required for Xis-integrase cooperativity but not for DNA binding. EMBO J. 11 3797-806 PubMed
  3. ↑ Sam, MD et al. (2004) Crystal structure of the excisionase-DNA complex from bacteriophage lambda. J. Mol. Biol. 338 229-40 PubMed

Categories

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