Phage lambda int:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

int

Synonyms

int, lambdap33

Product description

integration protein

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008979

prophage integrase activity

PMID:6444632[1]

IDA: Inferred from Direct Assay

F

Fig 6.

complete

GO:0008979

prophage integrase activity

PMID:1055366[2]

IDA: Inferred from Direct Assay

F

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Phage lambda Xis

PMID:1396573[3]

See Fig 4.

Protein

E. coli FIS

PMID:1396573[3]

see Fig 4.

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Notes

Spermidine biases resolution [4]

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MGRRRSHERR DLPPNLYIRN NGYYCYRDPR TGKEFGLGRD RRIAITEAIQ ANIELFSGHK
HKPLTARINS DNSVTLHSWL DRYEKILASR GIKQKTLINY MSKIKAIRRG LPDAPLEDIT
TKEIAAMLNG YIDEGKAASA KLIRSTLSDA FREAIAEGHI TTNHVAATRA AKSEVRRSRL
TADEYLKIYQ AAESSPCWLR LAMELAVVTG QRVGDLCEMK WSDIVDGYLY VEQSKTGVKI
AIPTALHIDA LGISMKETLD KCKEILGGET IIASTRREPL SSGTVSRYFM RARKASGLSF
EGDPPTFHEL RSLSARLYEK QISDKFAQHL LGHKSDTMAS QYRDDRGREW DKIEIK
Length

356

Mol. Wt

40.305 kDa

pI

9.8 (calculated)

Extinction coefficient

42,860 - 43,360 (calc based on 14 Y, 4 W, and 4 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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<motif_map/>

Structure
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Structures

<beststructure> gene=Phage_lambda_int taxon=10710 </beststructure>

Models

View models at:

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</protect>

Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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Name URL Comments
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References

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  1. ↑ Kotewicz, M et al. (1980) Purification and characterization of the integration protein specified by bacteriophage lambda. J. Biol. Chem. 255 2433-9 PubMed
  2. ↑ Nash, HA (1975) Integrative recombination of bacteriophage lambda DNA in vitro. Proc. Natl. Acad. Sci. U.S.A. 72 1072-6 PubMed
  3. ↑ 3.0 3.1 Numrych, TE et al. (1992) Characterization of the bacteriophage lambda excisionase (Xis) protein: the C-terminus is required for Xis-integrase cooperativity but not for DNA binding. EMBO J. 11 3797-806 PubMed
  4. ↑ Boldt, JL et al. (2007) Spermidine biases the resolution of Holliday junctions by phage lambda integrase. Nucleic Acids Res. 35 716-27 PubMed

Categories

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