mscL:Gene: Difference between revisions

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Table edited by Jtownley via TableEdit
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Table edited by Jtownley via TableEdit
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''mscL-Q56C''
''mscL-Q56C''
|
|
TGC
-->TGC
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|
Q56C
Q56C
Line 323: Line 323:
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|
Increased channel open time
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
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''mscL-Q56F''
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-->TTT
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Q56F
|
Conductance
|
Increased open channel time
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PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
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Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
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Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
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-->TAC
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Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
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Parental: AW405
|
See table 1
|-
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''mscL-Q56L''
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-->TTA
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Q56L
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No significant change in pressure sensitivity or open time.
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PMID:8876191
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Parental: AW405
|
See table 1
|-
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''mscL-Q56R''
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-->CGT
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Q56R
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Sensitivity to
|
Pressure
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PMID:8876191
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Parental: AW405
|
See table 1
|-
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''mscLΔ2-4''
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Deletion
|
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No significant change in pressure sensitivity or open time.
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PMID:8876191
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Parental: AW405
|
See table 1
|-
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''mscL-ΔQ56''
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Deletion
|
|
Sensitivity to
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Pressure
|
|
PMID:8876191
PMID:8876191

Revision as of 16:33, 6 November 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mscL

Mnemonic

Mechanosensitive channel

Synonyms

ECK3277, b3291, JW3252, yhdC[1], stkA, yhdC

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.06 minutes 

MG1655: 3436046..3436456
<gbrowseImage> name=NC_000913:3436046..3436456 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3365929..3366339
<gbrowseImage> name=NC_012967:3365929..3366339 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3323203..3323613
<gbrowseImage> name=NC_012759:3323203..3323613 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4202392..4201982
<gbrowseImage> name=NC_007779:4201982..4202392 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3533791..3534201
<gbrowseImage> name=NC_010473:3533791..3534201 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3436046

Edman degradation

PMID:7511799[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmscL (Keio:JW3252)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10453[4]

ΔmscL727::kan

PMID:16738554[3]

CGSC:101954

mscL-K31C

-->TGC

K31C

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See Table 1

mscL-K31R

-->AGG

K31R

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental:AW405

See table 1

mscL-K31E

-->GAG

K31E

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental:AW405

See table 1

mscL-Q56P

-->CCT

Q56P

Sensitivity to

Pressure

PMID:8876191[5]

Parental:AW405

see table 1

mscL-Q56C

-->TGC

Q56C

Conductance

Increased channel open time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56F

-->TTT

Q56F

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56W

-->TGG

Q56W

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

see table 1

mscL-Q56Y

-->TAC

Q56Y

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56L

-->TTA

Q56L

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56R

-->CGT

Q56R

Sensitivity to

Pressure

PMID:8876191[5]

Parental: AW405

See table 1

mscLΔ2-4

Deletion

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See table 1

mscL-ΔQ56

Deletion

Sensitivity to

Pressure

PMID:8876191[5]

Parental: AW405

See table 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3252

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[4]

est. P1 cotransduction: 78% [7]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 2% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11180

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11180

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001162

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947787

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1167

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010790

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Sukharev, SI et al. (1994) A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature 368 265-8 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 Blount, P et al. (1996) Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 11652-7 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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