uvrY:Gene: Difference between revisions

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''uvrY760(del)''::FRT
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Mutagenesis rate
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Decreased stress-induced mutagenesis (SIM) phenotype.
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PMID:23224554
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Parental Strain: SMR4562
Experimental Strain: SMR12314
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Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.


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Revision as of 22:47, 10 January 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uvrY

Mnemonic

UV resistant

Synonyms

ECK1913, b1914, JW1899, yecB[1], gacA, sirA, yecB


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

42.95 minutes 

MG1655: 1993383..1992727
<gbrowseImage> name=NC_000913:1992727..1993383 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1973205..1972696
<gbrowseImage> name=NC_012967:1972696..1973205 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1885210..1885866
<gbrowseImage> name=NC_012759:1885210..1885866 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1997496..1996840
<gbrowseImage> name=AC_000091:1996840..1997496 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2084391..2083735
<gbrowseImage> name=NC_010473:2083735..2084391 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

uvrY(del) (Keio:JW1899)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC9580[3]

uvrY1310(del)

PMID:12897016[4]

CGSC:82175

uvrY1362(del)

CGSC:82180

uvrY1296(del)

PMID:12366850[5]

CGSC:89616

uvrY760(del)::kan

PMID:16738554[2]

CGSC:103350

uvrY760(del)::FRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554[6]

Parental Strain: SMR4562

Experimental Strain: SMR12314

Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1899

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCATCAACGTTCTACTTGTTGA

Primer 2:CCtTGACTTGATAATGTCTCCGC

16B12

Kohara Phage

Genobase

PMID:3038334[8]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[3]

est. P1 cotransduction: 93% [9]

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[3]

est. P1 cotransduction: 14% [9]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG11140

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG11140

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946424

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Zhou, L et al. (2003) Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems. J. Bacteriol. 185 4956-72 PubMed
  5. ↑ Oshima, T et al. (2002) Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12. Mol. Microbiol. 46 281-91 PubMed
  6. ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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