uvrB:Gene: Difference between revisions

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PMID:6224549
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[[cgscmutation:9287|CGSC:9287]]
[[cgscmutation:9287|CGSC:9287]]

Revision as of 17:40, 24 October 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uvrB

Mnemonic

UV resistant

Synonyms

ECK0768, b0779, JW0762, dar-1, dar-6, visB[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.52 minutes 

MG1655: 812749..814770
<gbrowseImage> name=NC_000913:812749..814770 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 806152..808173
<gbrowseImage> name=NC_012967:806152..808173 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 715717..717738
<gbrowseImage> name=NC_012759:715717..717738 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 813948..815969
<gbrowseImage> name=AC_000091:813948..815969 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 866677..868698
<gbrowseImage> name=NC_010473:866677..868698 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

812752

Edman degradation

PMID:3515321[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔuvrB (Keio:JW0762)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC8819[5]

uvrBYY95AA

YY95AA

Defective in DNA-unwinding activity

seeded from UniProt:P0A8F8

uvrBY101A

Y101A

Defective in DNA-unwinding activity; when associated with A-108

seeded from UniProt:P0A8F8

uvrBF108A

F108A

Defective in DNA-unwinding activity; when associated with A-101

seeded from UniProt:P0A8F8

uvrB45

CGSC:9252

uvrB16

CGSC:9285

uvrB5

PMID:6224549[6]

CGSC:9287

uvrB27

CGSC:9324

uvrB29

CGSC:9326

uvrB30

CGSC:9328

uvrB501

CGSC:11315

uvrB151

CGSC:11965

uvrB153

CGSC:14433

uvrB230

CGSC:15657

ΔuvrB751::kan

PMID:16738554[4]

CGSC:103344



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0762

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAGTAAACCGTTCAAACTGAA

Primer 2:CCCGATGCCGCGATAAACAGCTC

4H7

Kohara Phage

Genobase

PMID:3038334[8]

3D4

Kohara Phage

Genobase

PMID:3038334[8]

24F9

Kohara Phage

Genobase

PMID:3038334[8]

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[5]

est. P1 cotransduction: 26% [9]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[5]

est. P1 cotransduction: 75% [9]
Synonyms:zbi-29::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG11062

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG11062

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945385

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Arikan, E et al. (1986) Sequences of the E. coli uvrB gene and protein. Nucleic Acids Res. 14 2637-50 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Drapeau, GR et al. (1983) Unusual properties of a new division mutant of Escherichia coli. Can. J. Microbiol. 29 694-9 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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