recG:Gene: Difference between revisions

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Primer 2:CCgGCATTCGAGTAACGTTCCGT  
Primer 2:CCgGCATTCGAGTAACGTTCCGT  
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[[:Category:Kohara_Phage:7F3|7F3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=571 Genobase]
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PMID:3038334
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est. P1 cotransduction: 86% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 86% <ref name='LIB:Nichols_linked_Tn'/>  
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[[:Category:Kohara_Phage:7F3|7F3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=571 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10829 EcoCyc:EG10829]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0822 EchoBASE:EB0822]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b3652 EcoliGenExpDB:b3652]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C4477 ECOL199310CYC:C4477]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10829 EcoGene:EG10829]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=RECG ECOO157CYC:RECG]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/307 CGSC:307]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0011939&formSubmitReturn=1 ASAP:ABE-0011939]
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Revision as of 21:16, 21 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recG

Mnemonic

Recombination

Synonyms

ECK3642, b3652, JW3627, spoV, radC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

82.4 minutes 

MG1655: 3823233..3825314
<gbrowseImage> name=NC_000913:3823233..3825314 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3763567..3765648
<gbrowseImage> name=NC_012967:3763567..3765648 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3711566..3713647
<gbrowseImage> name=NC_012759:3711566..3713647 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3815205..3813124
<gbrowseImage> name=AC_000091:3813124..3815205 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3920817..3922898
<gbrowseImage> name=NC_010473:3920817..3922898 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecG (Keio:JW3627)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10665[3]

recG::Tn5KAN-I-SceI (FB21370)

Insertion at nt 155 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21370

contains pKD46

recG::Tn5KAN-I-SceI (FB21371)

Insertion at nt 155 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21371

does not contain pKD46

recG

Deletion

recG2102

CGSC:13916

recG162

CGSC:26965

recG263(::kan)

PMID:8648624[5]

CGSC:69150

recG265(::cat)

CGSC:69152

ΔrecG756::kan

PMID:16738554[2]

CGSC:102626


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3627

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAGGTCGCCTGTTAGATGC

Primer 2:CCgGCATTCGAGTAACGTTCCGT

7F3

Kohara Phage

Genobase

PMID:3038334[7]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[3]

est. P1 cotransduction: % [8]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[3]

est. P1 cotransduction: 86% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Mahdi, AA et al. (1996) Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82. J. Mol. Biol. 257 561-73 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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