appB:Gene: Difference between revisions

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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=224 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=224 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:4H11|4H11]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=225 Genobase]
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PMID:3038334  
PMID:3038334  
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est. P1 cotransduction: 41% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 41% <ref name='LIB:Nichols_linked_Tn'/>  
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[[:Category:Kohara_Phage:4H11|4H11]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=225 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11379 EcoCyc:EG11379]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1353 EchoBASE:EB1353]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0979 EcoliGenExpDB:b0979]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C1120 ECOL199310CYC:C1120]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11379 EcoGene:EG11379]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=APPB ECOO157CYC:APPB]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/31813 CGSC:31813]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003302&formSubmitReturn=1 ASAP:ABE-0003302]
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Revision as of 19:30, 21 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

appB

Mnemonic

Acid (poly)phosphatase

Synonyms

ECK0970, b0979, JW0961, cyxB, cbdB[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

22.38 minutes 

MG1655: 1038519..1039655
<gbrowseImage> name=NC_000913:1038519..1039655 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1056387..1057523
<gbrowseImage> name=NC_012967:1056387..1057523 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 941487..942623
<gbrowseImage> name=NC_012759:941487..942623 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1039718..1040854
<gbrowseImage> name=AC_000091:1039718..1040854 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1092447..1093583
<gbrowseImage> name=NC_010473:1092447..1093583 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔappB (Keio:JW0961)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8957[3]

appB::Tn5KAN-2 (FB20079)

Insertion at nt 1071 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20079

does not contain pKD46

ΔappB::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938[5]

ΔappB::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[5]

ΔappB722::kan

PMID:16738554[2]

CGSC:100018



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0961

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTTTGATTATGAAACATTGCG

Primer 2:CCGTACAACTCGTTTTCGTTACG

4H9

Kohara Phage

Genobase

PMID:3038334[7]

4H11

Kohara Phage

Genobase

PMID:3038334[7]

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[3]

est. P1 cotransduction: 23% [8]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[3]

est. P1 cotransduction: 41% [8]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ 5.0 5.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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