prlC:Gene: Difference between revisions

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Primer 2:CCGCCCTTAATGCCGTAATGCTC  
Primer 2:CCGCCCTTAATGCCGTAATGCTC  
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[[:Category:Kohara_Phage:12E4|12E4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=608 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:12E4|12E4]]
[[:Category:Kohara_Phage:6A4|6A4]]
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Kohara Phage
Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=608 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=609 Genobase]
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PMID:3038334  
PMID:3038334  

Revision as of 21:35, 2 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

prlC

Mnemonic

Protein localization

Synonyms

ECK3483, b3498, JW3465, opdA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

78.48 minutes 

MG1655: 3643205..3641163
<gbrowseImage> name=NC_000913:3641163..3643205 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3575872..3573830
<gbrowseImage> name=NC_012967:3573830..3575872 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3529657..3531699
<gbrowseImage> name=NC_012759:3529657..3531699 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3995233..3997275
<gbrowseImage> name=AC_000091:3995233..3997275 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3740950..3738908
<gbrowseImage> name=NC_010473:3738908..3740950 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3641166

Edman degradation

PMID:9792922[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔprlC (Keio:JW3465)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10567[4]

prlC::Tn5KAN-I-SceI (FB21240)

Insertion at nt 1400 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB21240

contains pKD46

prlC::Tn5KAN-I-SceI (FB21241)

Insertion at nt 1400 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB21241

does not contain pKD46

ΔprlC754::kan

PMID:16738554[3]

CGSC:102462


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3465

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCACGAATCCGTTACTGACTCC

Primer 2:CCGCCCTTAATGCCGTAATGCTC

12E4

Kohara Phage

Genobase

PMID:3038334[7]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: 10% [8]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: % [8]

6A4

Kohara Phage

Genobase

PMID:3038334[7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11441

EchoBASE (EcoliWiki Page)

EchoBASE:EB1411

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3498

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4297

EcoGene (EcoliWiki Page)

EcoGene:EG11441

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PRLC

CGSC (EcoliWiki Page)

CGSC:18031

ASAP (EcoliWikiPage)

ASAP:ABE-0011421


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Jiang, X et al. (1998) Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle. J. Biochem. 124 980-5 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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