narI:Gene: Difference between revisions

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Primer 2:CCGTGACGAGCGCGCACCAGCTG  
Primer 2:CCGTGACGAGCGCGCACCAGCTG  
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[[:Category:Kohara_Phage:12H9|12H9]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=249 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:12H9|12H9]]
[[:Category:Kohara_Phage:4D8|4D8]]
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Kohara Phage
Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=249 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=250 Genobase]
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PMID:3038334  
PMID:3038334  

Revision as of 20:34, 2 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

narI

Mnemonic

Nitrate reductase, nitrate regulation

Synonyms

ECK1221, b1227, JW1218, chlI[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.7 minutes 

MG1655: 1285072..1285749
<gbrowseImage> name=NC_000913:1285072..1285749 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1285999..1286676
<gbrowseImage> name=NC_012967:1285999..1286676 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1173793..1174470
<gbrowseImage> name=NC_012759:1173793..1174470 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1287426..1288103
<gbrowseImage> name=AC_000091:1287426..1288103 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1325313..1325990
<gbrowseImage> name=NC_010473:1325313..1325990 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1285072

Edman degradation

PMID:3053688[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔnarI (Keio:JW1218)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9104[4]

ΔnarI738::kan

PMID:16738554[3]

CGSC:102022


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1218

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCAATTCCTGAATATGTTCTT

Primer 2:CCGTGACGAGCGCGCACCAGCTG

12H9

Kohara Phage

Genobase

PMID:3038334[6]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 9% [7]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 51% [7]
Synonyms:zch-506::Tn10, zci-506::Tn10

4D8

Kohara Phage

Genobase

PMID:3038334[6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10640

EchoBASE (EcoliWiki Page)

EchoBASE:EB0634

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1227

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1688

EcoGene (EcoliWiki Page)

EcoGene:EG10640

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NARI

CGSC (EcoliWiki Page)

CGSC:458

ASAP (EcoliWikiPage)

ASAP:ABE-0004125


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Sodergren, EJ et al. (1988) Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli. J. Biol. Chem. 263 16156-62 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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