recJ:Gene: Difference between revisions
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truncation | truncation | ||
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null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. | null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. | ||
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G to D amino acid 78 | G to D amino acid 78 | ||
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similar to recJ284, null | similar to recJ284, null | ||
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None | None | ||
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Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation | Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation | ||
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Revision as of 00:47, 16 August 2011
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
recJ |
|---|---|
| Mnemonic |
Recombination |
| Synonyms |
ECK2887, b2892, JW2860[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
65.4 minutes |
MG1655: 3036128..3034395 |
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NC_012967: 2923859..2922126 |
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NC_012759: 2921543..2923276 |
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|
W3110 |
|
W3110: 3036762..3035029 |
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DH10B: 3129998..3128265 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔrecJ (Keio:JW2860) |
deletion |
deletion |
|||||
|
recJ::Tn5KAN-I-SceI (FB20963) |
Insertion at nt 1026 in Minus orientation |
contains pKD46 | |||||
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recJ::Tn5KAN-I-SceI (FB20964) |
Insertion at nt 1026 in Minus orientation |
does not contain pKD46 | |||||
|
recJ284::Tn10 |
Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon |
truncation |
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. |
CGSC |
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|
recJ77 |
G to A nucleotide 281 |
G to D amino acid 78 |
similar to recJ284, null |
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|
recJ153 recJ154 |
GTG to GTA at start codon |
None |
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation |
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recJ153 |
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recJ284::Tn10 |
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ΔrecJ743::kan |
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| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2860 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAACAACAGATACAACTTCG Primer 2:CCAATTGGCCAGATATTGTCGAT | |
|
Linked marker |
est. P1 cotransduction: % [10] | ||
|
Linked marker |
est. P1 cotransduction: 9% [10] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 5.0 5.1 5.2 Lovett, ST & Clark, AJ (1984) Genetic analysis of the recJ gene of Escherichia coli K-12. J. Bacteriol. 157 190-6 PubMed
- ↑ Gillen, JR et al. (1981) Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J. Bacteriol. 145 521-32 PubMed
- ↑ Horii, Z & Clark, AJ (1973) Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J. Mol. Biol. 80 327-44 PubMed
- ↑ Haggerty, TJ & Lovett, ST (1997) IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli. J. Bacteriol. 179 6705-13 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).