chbF:Gene: Difference between revisions

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Fixing Blattner deletion strains in alleles table.
Adding section & Genetic interactions table to Gene pages.
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|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
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|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
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|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:17, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

chbF

Mnemonic

Chitobiose

Synonyms

ECK1732, b1734, JW1723, celF, ydjD[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

39.12 minutes 

MG1655: 1816524..1815172
<gbrowseImage> name=NC_000913:1815172..1816524 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1794413..1795765
<gbrowseImage> name=NC_012967:1794413..1795765 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1707231..1708583
<gbrowseImage> name=NC_012759:1707231..1708583 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1820214..1818862
<gbrowseImage> name=AC_000091:1818862..1820214 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1907095..1905743
<gbrowseImage> name=NC_010473:1905743..1907095 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1815175

Edman degradation

PMID:10572139[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔchbF (Keio:JW1723)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9455[4]

celF::Tn5KAN-2 (FB20441)

Insertion at nt 1164 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20441

contains pKD46

ΔchbF733::kan

PMID:16738554[3]

CGSC:100290



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1723

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGCCAGAAATTAAAAGTCGT

Primer 2:CCATGTGCTTTTTTAAGCTCTGC

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[4]

est. P1 cotransduction: 20% [7]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[4]

est. P1 cotransduction: 53% [7]
Synonyms:zdi-276::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10144

EchoBASE (EcoliWiki Page)

EchoBASE:EB0142

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1734

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2133

EcoGene (EcoliWiki Page)

EcoGene:EG10144

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CELF

CGSC (EcoliWiki Page)

CGSC:17734

ASAP (EcoliWikiPage)

ASAP:ABE-0005785



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Thompson, J et al. (1999) Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases. J. Bacteriol. 181 7339-45 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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