intD:Gene: Difference between revisions
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Fixing Blattner deletion strains in alleles table. |
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0001842 intD] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0001842 intD::Tn5KAN-2] (FB20199) | ||
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Insertion at nt 524 in Minus orientation | |||
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PMID: | PMID:15262929 | ||
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[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20199] | ||
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does not contain [[pKD46]] | |||
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Revision as of 21:47, 9 March 2011
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
intD |
|---|---|
| Mnemonic |
Integrase |
| Synonyms |
ECK0530, b0537, JW0525, int[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
12.16 minutes |
MG1655: 565201..564038 |
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NC_012967: 538092..536929 |
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NC_012759: 466798..467961 |
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W3110 |
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W3110: 565201..564038 |
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DH10B: 504533..503370 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔintD (Keio:JW0525) |
deletion |
deletion |
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|
intD::Tn5KAN-2 (FB20199) |
Insertion at nt 524 in Minus orientation |
does not contain pKD46 | |||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0525 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCACTCTTCCGCAGAAATGA Primer 2:CCCGCCTTCTTTATATCCTCCAT | |
|
Linked marker |
est. P1 cotransduction: 66% [6] | ||
|
Linked marker |
est. P1 cotransduction: 1% [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).