ppc:Gene: Difference between revisions
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109118 CGSC10837]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109118 CGSC10837]<ref name='LIB:cgsc'/> | ||
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Δppc::kan | |||
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deletion | |||
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[[Biolog]]:respiration | |||
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unable to respire [[Carbon sources|Dextrin]] | |||
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PMID:16095938 | |||
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[[Biolog]]:respiration | [[Biolog]]:respiration | ||
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unable to respire [[Carbon sources| | unable to respire [[Carbon sources|D-Sorbitol]] | ||
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PMID:16095938 | PMID:16095938 | ||
Revision as of 17:12, 26 April 2010
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ppc |
|---|---|
| Mnemonic |
PEP carboxylase |
| Synonyms |
ECK3947, b3956, JW3928, asp, glu, pepC[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
89.41 minutes, 89.41 minutes |
MG1655: 4151121..4148470 |
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NC_012967: 4133205..4130554 |
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W3110 |
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W3110: 3483583..3486234 |
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DH10B: 4250818..4248167 |
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NC_012759: 4038139..4040790 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ppcR587S |
R587S |
Loss of activity |
seeded from UniProt:P00864 | ||||
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ppcH579P |
H579P |
5.4% of wild-type activity |
seeded from UniProt:P00864 | ||||
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ppcH579N |
H579N |
29% of wild-type activity |
seeded from UniProt:P00864 | ||||
|
ppcH138N |
H138N |
Loss of activity |
seeded from UniProt:P00864 | ||||
|
Δppc (Keio:JW3928) |
deletion |
deletion |
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Δppc::kan |
deletion |
Biolog:respiration |
unable to respire Dextrin |
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ppc-2 |
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ppc-1 |
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ppc-3 |
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ppc-5::kan |
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Δppc-742::kan |
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|
Δppc::kan |
deletion |
Biolog:respiration |
unable to respire D-Sorbitol |
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| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3928 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAACGAACAATATTCCGCATT Primer 2:CCGCCGGTATTACGCATACCTGC | |
|
Linked marker |
est. P1 cotransduction: 84% [9] | ||
|
Linked marker |
est. P1 cotransduction: 88% [9] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
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| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ ADELBERG, EA & BURNS, SN (1960) Genetic variation in the sex factor of Escherichia coli. J. Bacteriol. 79 321-30 PubMed
- ↑ 6.0 6.1 Vanderwinkel, E & De Vlieghere, M (1968) [Physiology and genetics of isocitritase and the malate synthases of Escherichia coli]. Eur. J. Biochem. 5 81-90 PubMed
- ↑ 7.0 7.1 GLANSDORFF, N (1965) TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12. Genetics 51 167-79 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).