hrpB:Gene: Difference between revisions

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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106695 CGSC8414]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106695 CGSC8414]<ref name='LIB:cgsc'/>  
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&Delta;hrpB::kan
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deletion
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[[Biolog]]:respiration
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unable to respire [[Carbon sources|D-Mannose]]
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PMID:16095938
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[[Biolog]]:respiration
[[Biolog]]:respiration
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unable to respire [[Carbon sources|D-Mannose]]
unable to respire [[Carbon sources|Acetate]]
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PMID:16095938
PMID:16095938

Revision as of 16:55, 26 April 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hrpB

Mnemonic

Helicase-related protein

Synonyms

ECK0147, b0148, JW0144, yadO[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

3.49 minutes 

MG1655: 162105..164534
gbrowse_img.php?coord=162105..164534&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 164956..167385
gbrowse_img.php?coord=164956..167385&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 162105..164534
gbrowse_img.php?coord=162105..164534&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 136209..138638
gbrowse_img.php?coord=136209..138638&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 162104..164533
gbrowse_img.php?coord=162104..164533&genome=BW2952&name=NC_012759&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhrpB (Keio:JW0144)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8414[3]

ΔhrpB::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938[4]

ΔhrpB764::kan

PMID:16738554[2]

CGSC:101406

ΔhrpB::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[4]



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0144

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCGTCGTTGCCCGTTGCTGC

Primer 2:CCCGAATACTTTTTCGTCCGTCG

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[3]

est. P1 cotransduction: 62% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[3]

est. P1 cotransduction: 2% [6]
Synonyms:zae-502::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12329

EchoBASE (EcoliWiki Page)

EchoBASE:EB2233

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0148

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0181

EcoGene (EcoliWiki Page)

EcoGene:EG12329

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HRPB

CGSC (EcoliWiki Page)

CGSC:36926

ASAP (EcoliWikiPage)

ASAP:ABE-0000510



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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