hrpB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HrpB

Synonyms

predicted ATP-dependent helicase[1], B0148[2][1], YadO[2][1], HrpB[2][1] , ECK0147, JW0144, yadO, b0148

Product description

predicted ATP-dependent helicase[2][3]

RNA helicase-like; similarity to eukaryotic DEAH family and araC[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007502

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0008026

ATP-dependent helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011545

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceF

PMID:15690043[5]

Experiment(s):EBI-884833

Protein

lpdA

PMID:15690043[5]

Experiment(s):EBI-884833, EBI-891778

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-891778

Protein

dnaK

PMID:16606699[6]

Experiment(s):EBI-1135720

Protein

rplL

PMID:19402753[7]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLQCGAKNVN PLERFVSSLP VAAVLPELLT ALDCAPQVLL SAPTGAGKST WLPLQLLAHP
GINGKIILLE PRRLAARNVA QRLAELLNEK PGDTVGYRMR AQNCVGPNTR LEVVTEGVLT
RMIQRDPELS GVGLVILDEF HERSLQADLA LALLLDVQQG LRDDLKLLIM SATLDNDRLQ
QMLPEAPVVI SEGRSFPVER RYLPLPAHQR FDDAVAVATA EMLRQESGSL LLFLPGVGEI
QRVQEQLASR IGSDVLLCPL YGALSLNDQR KAILPAPQGM RKVVLATNIA ETSLTIEGIR
LVVDCAQERV ARFDPRTGLT RLITQRVSQA SMTQRAGRAG RLEPGISLHL IAKEQAERAA
AQSEPEILQS DLSGLLMELL QWGCSDPAQM SWLDQPPVVN LLAAKRLLQM LGALEGERLS
AQGQKMAALG NDPRLAAMLV SAKNDDEAAT AAKIAAILEE PPRMGNSDLG VAFSRNQPAW
QQRSQQLLKR LNVRGGEADS SLIAPLLAGA FADRIARRRG QDGRYQLANG MGAMLDANDA
LSRHEWLIAP LLLQGSASPD ARILLALLVD IDELVQRCPQ LVQQSDTVEW DDAQGTLKAW
RRLQIGQLTV KVQPLAKPSE DELHQAMLNG IRDKGLSVLN WTAEAEQLRL RLLCAAKWLP
EYDWPAVDDE SLLAALETWL LPHMTGVHSL RGLKSLDIYQ ALRGLLDWGM QQRLDSELPA
HYTVPTGSRI AIRYHEDNPP ALAVRMQEMF GEATNPTIAQ GRVPLVLELL SPAQRPLQIT
RDLSDFWKGA YREVQKEMKG RYPKHVWPDD PANTAPTRRT KKYS
Length

824

Mol. Wt

90.819 kDa

pI

6.6 (calculated)

Extinction coefficient

93,390 - 94,390 (calc based on 11 Y, 14 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

7..167

PF00270 DEAD/DEAH box helicase

PMID:19920124[8]

Domain

388..504

PF04408 Helicase associated domain (HA2)

PMID:19920124[8]

Domain

237..328

PF00271 Helicase conserved C-terminal domain

PMID:19920124[8]

Domain

664..796

PF08482 ATP-dependent helicase C-terminal

PMID:19920124[8]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hrpB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:162135893

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944845

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000510

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P37024

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12329

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12329

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944845

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002204

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2233

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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