lexA:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Standard name
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''lexA''
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!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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Lambda excision
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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK4035, b4043, JW4003, exrA, tsl, umuA''<ref name='LIB:Riley_2006'/>


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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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!|Feature type!!Location!!Description
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uninducible, not cleaved during SOS response  
uninducible, not cleaved during SOS response  
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lexAG85D
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G85D
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(in lexA3, resistant to cleavage)
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P0A7C2 UniProt:P0A7C2]
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lexAG85D
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G85D
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(in lexA3, resistant to cleavage)
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P0A7C2 UniProt:P0A7C2]


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Revision as of 21:22, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lexA

Mnemonic

Lambda excision

Synonyms

ECK4035, b4043, JW4003, exrA, tsl, umuA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.71 minutes 

MG1655: 4255138..4255746
gbrowse_img.php?coord=4255138..4255746&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4236047..4236655
gbrowse_img.php?coord=4236047..4236655&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4260705..4261313
gbrowse_img.php?coord=4260705..4261313&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4354834..4355442
gbrowse_img.php?coord=4354834..4355442&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

spr

PMID:2834329[2] PMID:7038393[3]

uninducible, not cleaved during SOS response

lexAG85D

G85D

(in lexA3, resistant to cleavage)

Strain variation; seeded from UniProt:P0A7C2

lexA107

CGSC:6267

lexA1(Ind-)

non-inducible SOS response

CGSC:9237

lexA41(ts)

temperature sensitive

CGSC:9601

lexA3(Ind-)

non-inducible SOS response

CGSC:9602

lexA51

CGSC:10280

lexA101

CGSC:11964

lexA102

CGSC:11979

lexA109

CGSC:11985

lexA103

CGSC:14425

lexA104

CGSC:14427

lexA105

CGSC:14429

lexA106

CGSC:14431

lexA108

CGSC:14439

lexA71::Tn5

CGSC:30813


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4003

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAAAGCGTTAACGGCCAGGCA

Primer 2:CCaAGCCAGTCGCCGTTGCGAAT

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[5]

est. P1 cotransduction: 60% [6]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[5]

est. P1 cotransduction: 9% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10533

EchoBASE (EcoliWiki Page)

EchoBASE:EB0528

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4043

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5014

EcoGene (EcoliWiki Page)

EcoGene:EG10533

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:LEXA

CGSC (EcoliWiki Page)

CGSC:558

ASAP (EcoliWikiPage)

ASAP:ABE-0013241


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Lin, LL & Little, JW (1988) Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170 2163-73 PubMed
  3. ↑ Froehlich, B (1981) Weigle reactivation of the single-stranded DNA phage f1. Mol. Gen. Genet. 184 416-20 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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