hrpA:Gene Product(s): Difference between revisions

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!align=left |Standard name
!align=left {{table heading style}} |Standard name
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HrpA  
HrpA  
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!align=left |Synonyms
!align=left {{table heading style}} |Synonyms
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ATP-dependent helicase<ref name='LIB:Riley_2006'/>, B1413<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, HrpA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
ATP-dependent helicase<ref name='LIB:Riley_2006'/>, B1413<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, HrpA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
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!align=left |Product description
!align=left {{table heading style}} |Product description
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predicted ATP-dependent helicase, involved in mRNA processing<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
predicted ATP-dependent helicase, involved in mRNA processing<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
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RNA helicase-like; similarity to eukaryotic DEAH family<ref name='LIB:EcoGene'/>
RNA helicase-like; similarity to eukaryotic DEAH family<ref name='LIB:EcoGene'/>
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!align=left |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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!|Partner Type!!Partner!!Notes!!References!!Evidence
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!|Compartment!!Description!!Evidence!!Source!!Notes
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|-
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!align=left |Sequence
!align=left {{table heading style}} |Sequence
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[http://biocyc.org/ECOLI/sequence-aa?object=G6732-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=G6732-MONOMER at EcoCyc]  
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LGTPYPISDK RILQAMEQIS G</pre>  
LGTPYPISDK RILQAMEQIS G</pre>  
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!align=left |Length
!align=left {{table heading style}} |Length
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1,281
1,281
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!align=left |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
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146.798 kDa
146.798 kDa
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!align=left |pI
!align=left {{table heading style}} |pI
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8.0 (calculated)
8.0 (calculated)
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!align=left |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
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160,660 - 161,785 (calc based on 34 Y, 20 W, and 9 C residues)
160,660 - 161,785 (calc based on 34 Y, 20 W, and 9 C residues)
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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References


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!align=left |Structures
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<beststructure>
<beststructure>
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</beststructure>
</beststructure>
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!align=left |Models
!align=left {{table heading style}} |Models
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View models at:
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!|Resource type!!Source!!Notes/Reference
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!|Name!!URL!!Comments
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Revision as of 20:07, 23 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HrpA

Synonyms

ATP-dependent helicase[1], B1413[2][1], HrpA[2][1]

Product description

predicted ATP-dependent helicase, involved in mRNA processing[2][3]

RNA helicase-like; similarity to eukaryotic DEAH family[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0006397

mRNA processing

PMID:15186427[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [6]

complete

GO:0004004

ATP-dependent RNA helicase activity

PMID:7899078[7]

ISS: Inferred from Sequence or Structural Similarity

F

Seeded from EcoCyc [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003593
SP_KW:KW-0547

F

Seeded from EcoCyc [6]

complete

GO:0003676

nucleic acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001650

F

Seeded from EcoCyc [6]

complete

GO:0004386

helicase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001650
InterPro:IPR007502
SP_KW:KW-0347

F

Seeded from EcoCyc [6]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001650
InterPro:IPR010222
SP_KW:KW-0067

F

Seeded from EcoCyc [6]

complete

GO:0008026

ATP-dependent helicase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010222

F

Seeded from EcoCyc [6]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [6]

complete

GO:0017111

nucleoside-triphosphatase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc [6]

complete

GO:0009451

RNA modification

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceE

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

aceF

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

lpdA

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rplI

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsA

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsC

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsE

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsG

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsM

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

rpsS

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

tufA

PMID:15690043[8]

Experiment(s):EBI-884859

Protein

yfiF

PMID:15690043[8]

Experiment(s):EBI-884859
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MLRDRLRFSR RLHGVKKVKN PDAQQAIFQE MAKEIDQAAG KVLLREAARP EITYPDNLPV
SQKKQDILEA IRDHQVVIVA GETGSGKTTQ LPKICMELGR GIKGLIGHTQ PRRLAARTVA
NRIAEELKTE PGGCIGYKVR FSDHVSDNTM VKLMTDGILL AEIQQDRLLM QYDTIIIDEA
HERSLNIDFL LGYLKELLPR RPDLKIIITS ATIDPERFSR HFNNAPIIEV SGRTYPVEVR
YRPIVEEADD TERDQLQAIF DAVDELSQES HGDILIFMSG EREIRDTADA LNKLNLRHTE
ILPLYARLSN SEQNRVFQSH SGRRIVLATN VAETSLTVPG IKYVIDPGTA RISRYSYRTK
VQRLPIEPIS QASANQRKGR CGRVSEGICI RLYSEDDFLS RPEFTDPEIL RTNLASVILQ
MTALGLGDIA AFPFVEAPDK RNIQDGVRLL EELGAITTDE QASAYKLTPL GRQLSQLPVD
PRLARMVLEA QKHGCVREAM IITSALSIQD PRERPMDKQQ ASDEKHRRFH DKESDFLAFV
NLWNYLGEQQ KALSSNAFRR LCRTDYLNYL RVREWQDIYT QLRQVVKELG IPVNSEPAEY
REIHIALLTG LLSHIGMKDA DKQEYTGARN ARFSIFPGSG LFKKPPKWVM VAELVETSRL
WGRIAARIDP EWVEPVAQHL IKRTYSEPHW ERAQGAVMAT EKVTVYGLPI VAARKVNYSQ
IDPALCRELF IRHALVEGDW QTRHAFFREN LKLRAEVEEL EHKSRRRDIL VDDETLFEFY
DQRISHDVIS ARHFDSWWKK VSRETPDLLN FEKSMLIKEG AEKISKLDYP NFWHQGNLKL
RLSYQFEPGA DADGVTVHIP LPLLNQVEES GFEWQIPGLR RELVIALIKS LPKPVRRNFV
PAPNYAEAFL GRVKPLELPL LDSLERELRR MTGVTVDRED WHWDQVPDHL KITFRVVDDK
NKKLKEGRSL QDLKDALKGK VQETLSAVAD DGIEQSGLHI WSFGQLPESY EQKRGNYKVK
AWPALVDERD SVAIKLFDNP LEQKQAMWNG LRRLLLLNIP SPIKYLHEKL PNKAKLGLYF
NPYGKVLELI DDCISCGVDK LIDANGGPVW TEEGFAALHE KVRAELNDTV VDIAKQVEQI
LTAVFNINKR LKGRVDMTMA LGLSDIKAQM GGLVYRGFVT GNGFKRLGDT LRYLQAIEKR
LEKLAVDPHR DRAQMLKVEN VQQAWQQWIN KLPPARREDE DVKEIRWMIE ELRVSYFAQQ
LGTPYPISDK RILQAMEQIS G
Length

1,281

Mol. Wt

146.798 kDa

pI

8.0 (calculated)

Extinction coefficient

160,660 - 161,785 (calc based on 34 Y, 20 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hrpA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6732-MONOMER

UniProt (EcoliWiki Page)

UniProt:P43329

PFAM (EcoliWiki Page)

PFAM:PF00270

RefSeq (EcoliWiki Page)

RefSeq:NP_415931


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Koo, JT et al. (2004) HrpA, a DEAH-box RNA helicase, is involved in mRNA processing of a fimbrial operon in Escherichia coli. Mol. Microbiol. 52 1813-26 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ Moriya, H et al. (1995) Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae. Nucleic Acids Res. 23 595-8 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed

Categories

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