PMID:7899078
| Citation |
Moriya, H, Kasai, H and Isono, K (1995) Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae. Nucleic Acids Res. 23:595-8 |
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| Abstract |
During the course of systematic nucleotide sequence analysis of the terC region of E.coli K-12 by using the ordered lambda phage clones, we found the presence of a gene, termed hrpA, that showed a high degree of sequence similarity to the PRP2, PRP16 and PRP22 genes of Saccharomyces cerevisiae. The products of these yeast genes are known to play their roles in mRNA splicing, and belong to a group of proteins collectively called the DEAH family. The hrpA gene is the first example of a DEAH family gene in prokaryotes. The N-terminal region of the protein it encodes contains conserved sequence stretches characteristic of an RNA helicase. Its molecular mass is calculated to be 146 kDa. Previously, a 135 kDa protein was identified by Moir et al. [J. Bacteriol. (1992) 174, 2102-2110] in this region which is most likely identical to that encoded by hrpA. The C-terminal region of the hrpA gene product seems to contain an RNA binding motif weakly resembling that of ribosomal protein S1 of E.coli. Disruption of the hrpA gene suggested that it is not essential for the growth of E.coli. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DEAD-box RNA Helicases; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Fungal Proteins/genetics; Genes, Bacterial; Genes, Fungal; Molecular Sequence Data; Multigene Family; RNA Helicases; RNA Nucleotidyltransferases/genetics; RNA Splicing; Regulatory Sequences, Nucleic Acid; Repressor Proteins/genetics; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Homology; Transcription Factors |
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