ftsA:Expression: Difference between revisions

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*Medium:  LB broth
*Medium:  LB broth
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 1.67  
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.67  
*other:
*Growth phase: other:
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quantitative western
quantitative western
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*Medium:  LB + 0.4% Glucose
*Medium:  LB + 0.4% Glucose
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 2.00
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 2.00
*other:
*Growth phase: other:
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quantitative western
quantitative western
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*Medium:  M9 + 1% casamino acids + 0.4% glucose
*Medium:  M9 + 1% casamino acids + 0.4% glucose
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 1.62  
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.62  
*other:
*Growth phase: other:
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quantitative western
quantitative western
Line 111: Line 111:
*Medium:  M9 + 1% casamino acids + 0.4% glycerol
*Medium:  M9 + 1% casamino acids + 0.4% glycerol
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 1.03
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.03
*other:
*Growth phase: other:
|
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quantitative western
quantitative western
Line 131: Line 131:
*Medium:  M9 + 0.4% glucose
*Medium:  M9 + 0.4% glucose
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 0.52
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.52
*other:
*Growth phase: other:
|
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quantitative western
quantitative western
Line 151: Line 151:
*Medium:  M9 + 0.4% glycerol
*Medium:  M9 + 0.4% glycerol
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 0.48
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.48
*other:
*Growth phase: other:
|
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quantitative western
quantitative western
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*Medium:  M9 + 0.4% succinate
*Medium:  M9 + 0.4% succinate
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 0.44  
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.44  
*other:
*Growth phase: other:
|
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quantitative western
quantitative western
Line 191: Line 191:
*Medium:  M9 + 0.4% acetate
*Medium:  M9 + 0.4% acetate
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):  Doubling time (per hour) = 0.41
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.41
*other:
*Growth phase: other:
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quantitative western
quantitative western
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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PMID:20671182  
PMID:20671182  
|-
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Protein
|
''E. coli'' K-12 MG1655
|
984
|
molecules/cell/generation
|
*Medium: MOPS Complete 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
575
|
molecules/cell/generation
|
*Medium: MOPS Minimal 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
1013
|
molecules/cell/generation
|
*Medium: MOPS Complete without Methionine 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808


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Latest revision as of 19:38, 21 July 2015

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli D1210

175

molecules/cell

  • Medium: LB broth
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.67
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

200

molecules/cell

  • Medium: LB + 0.4% Glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 2.00
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

127

molecules/cell

  • Medium: M9 + 1% casamino acids + 0.4% glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.62
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

100

molecules/cell

  • Medium: M9 + 1% casamino acids + 0.4% glycerol
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.03
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

74

molecules/cell

  • Medium: M9 + 0.4% glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.52
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

48

molecules/cell

  • Medium: M9 + 0.4% glycerol
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.48
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

45

molecules/cell

  • Medium: M9 + 0.4% succinate
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.44
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

43

molecules/cell

  • Medium: M9 + 0.4% acetate
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.41
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

Ecoli K-12

58.922+/-0.223

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182[2]

mRNA

Ecoli K-12

0.161648177

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182[2]

Protein

E. coli K-12 MG1655

984

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

575

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

1013

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ftsAZ

mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ

ftsQAZ

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Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Overproduction of FtsA causes long non-septate filaments to form and inhibits normal cell septation. [4]

Overproduction does block division but transcription from cloned fts promoters is not further repressed. [5]

RcsB effects the efficiency of the transcription of ftsA1p. Specifically, causing it too increase in efficiency as more of RcsB is added. [6]

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:103962..104002 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0094 (EcoliWiki Page)

NCBI GEO profiles for ftsA

microarray

GenExpDB:b0094 (EcoliWiki Page)

Summary of data for ftsA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to ftsA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10339

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0335

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0094

EcoGene

EcoGene:EG10339

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000333

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000331

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Wang, H & Gayda, RC (1992) Quantitative determination of FtsA at different growth rates in Escherichia coli using monoclonal antibodies. Mol. Microbiol. 6 2517-24 PubMed
  2. ↑ 2.0 2.1 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  3. ↑ 3.0 3.1 3.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
  4. ↑ Wang, HC & Gayda, RC (1990) High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12. J. Bacteriol. 172 4736-40 PubMed
  5. ↑ Dewar, SJ et al. (1989) Transcriptional regulation of cell division genes in Escherichia coli. Mol. Microbiol. 3 1371-7 PubMed
  6. ↑ Carballès, F et al. (1999) Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol. Microbiol. 34 442-50 PubMed

Categories

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