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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11035 ECOCYC:EG11035]
EcoCyc:EG11035
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11035 EcoGene:EG11035]
EcoGene:EG11035
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[RegulonDB]]
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RegulonDB:ECK120001024
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:EB1028
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0001425
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:12, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tsx

Mnemonic

T-six

Synonyms

ECK0405, b0411, JW0401, T6rec, nupA[1], nupA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.28 minutes 

MG1655: 431237..430353
<gbrowseImage> name=NC_000913:430353..431237 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 400435..399662
<gbrowseImage> name=NC_012967:399662..400435 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 333112..333996
<gbrowseImage> name=NC_012759:333112..333996 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 431237..430353
<gbrowseImage> name=AC_000091:430353..431237 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 370568..369684
<gbrowseImage> name=NC_010473:369684..370568 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tsx(del) (Keio:JW0401)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC8574[3]

tsxN276Y

N276Y

(in phage resistant mutant)

Strain variation; seeded from UniProt:P0A927

tsx-247::Tn10

Insertion at 430,667 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12148 = CGSC7337[3]

Synonyms:

tsx-33

CGSC:4496

tsx-29

CGSC:4846

tsx-67

CGSC:4930

tsx-1

CGSC:5240

tsx-70

CGSC:5297

tsx-358

CGSC:5309

tsx-81

CGSC:5575

tsx-76

CGSC:5602

tsx-79

CGSC:5608

tsx-84

CGSC:5663

tsx-95

CGSC:5684

tsx-7

CGSC:5796

tsx-63

CGSC:5830

tsx-78

CGSC:5945

tsx-96

CGSC:6184

tsx-71

CGSC:6370

tsx-64

CGSC:6458

tsx-273

CGSC:6598

tsx-97

CGSC:6623

tsx-0

CGSC:6729

tsx-68

CGSC:7094

tsx-463

CGSC:7525

tsx-352

CGSC:7563

tsx-83

CGSC:7609

tsx-80

CGSC:7845

tsx-82

CGSC:7912

tsx-65

CGSC:8002

tsx-354

CGSC:8220

tsx-356

CGSC:8224

tsx-357

CGSC:8227

tsx-3

CGSC:8257

tsx-61

CGSC:8309

tsx-59

CGSC:8355

tsx-6

CGSC:8384

tsx-274

CGSC:8505

tsx-87

CGSC:8763

tsx-23

CGSC:8896

tsx-25

CGSC:8897

tsx-464

CGSC:9000

tsx-36

CGSC:9084

tsx-57

CGSC:9107

tsx-5

CGSC:9357

tsx-353

CGSC:9453

tsx-462::Tn10

CGSC:9490

tsx-93

CGSC:9803

tsx-466

CGSC:9983

tsx-69

CGSC:10430

tsx-465(Am)

CGSC:10863

amber (UAG) mutation

tsx-72

CGSC:11129

tsx-21

CGSC:11149

tsx-2

CGSC:11151

tsx-85

CGSC:11842

tsx-460

CGSC:12214

tsx-18

CGSC:13122

tsx-17

CGSC:13168

tsx-27

CGSC:13213

tsx-4

CGSC:13243

tsx-58

CGSC:13378

tsx-62

CGSC:13948

tsx-73

CGSC:14208

tsx-459

CGSC:14332

tsx-19

CGSC:15762

tsx-37

CGSC:16094

tsx-77

CGSC:17075

tsx-247::Tn10

CGSC:39365

tsx-3100::Tn10kan

CGSC:39368

tsx-35

CGSC:43389

tsx-74

CGSC:72463

tsx-75

CGSC:72484

tsx-773(del)::kan

PMID:16738554[2]

CGSC:103248

tsx mutation in P1807

Sensitivity to

Reduced sensitivity toward albicidin.

PMID:2191080[5]

See Figure 1 B for full experimental results.

tsx mutation in strain P1807

Metabolic uptake

Mutation to the tsx region caused a reduced uptake of nucleoside.

PMID:2191080[5]

See figure 2 for full experimental results.

tsx mutation in strain GP4

Metabolic uptake

Mutation to the tsx region caused a reduced uptake of ablicidin.

PMID:2191080[5]

These results support the theory that ablicidin resistance is due to reduced uptake of the antibiotic. See figure 3b for experimental results.

tsx- in P407

Resistant to

Resistance to Bacteriophage T6

PMID:786267[6]

Mutation to the tsx gene confers resistance to phage T6, possibly by reducing the production of a protein thought to be involved in phage binding, tsx-protein.

tsx--con- in P1731

Resistant to

Resistance to Bacteriophage T6

PMID:786267[6]

A double mutation to the tsx and con gene confers resistance to phage T6, possibly by reducing the production of a protein thought to be involved in phage binding, tsx-protein.

tsx- in P407

Resistant to

Resistance to Colicin K

PMID:786267[6]

Mutation to the tsx gene confers resistance to Colicin K possibly by reducing the production of a specific protein, tsx-protein.

tsx--con- in P1731

Resistant to

Resistance to Colicin K

PMID:786267[6]

A double mutation to the tsx and con gene confers resistance to Colicin K, possibly by reducing the production of a protein thought to be involved in phage binding, tsx-protein.

tsx-2 in strain KG16

Resistant to

Resistance to strain Phage T6

PMID:6808961[7]

tsx-2 class of mutants were resistant to bacteriophage T6. This was also seen in strain KG19.

tsx-2 in strain KG16

Resistant to

Resistance to Colicin K

PMID:6808961[7]

tsx-2 class of mutants became more resistant to Colicin K. This was also seen in strain KG19.

tsx-1 in strain GG24

Resistant to

Resistance to strain Phage T6

PMID:6808961[7]

tsx-1 class of mutants developed an increased resistance toward bacteriophage T6. This was also seen in strain GG26.

tsx-1 in strain GG24d

Resistant to

Resistance to Colicin K

PMID:6808961[7]

tsx-2 class of mutants became more resistant to Colicin K. This was also seen in strain GG24.

tsx-2 in strain KG16

Growth Phenotype

Muciod growth

PMID:6808961[7]

It was found that the tsx-2 class of mutants produced a Muciod type growth configuration. This included strain KG19.

tsx-2 in strain KG16

Sensitivity to

Increased sensitivity to Neomycin.

PMID:6808961[7]

It was found that the tsx-2 class of mutants became increasingly sensitive to aminoglycosides Neomycin and Kanamycin. This included strain KG19.

tsx-2 in strain KG16

Sensitivity to

Increased sensitivity to Kanamycin.

PMID:6808961[7]

It was found that the tsx-2 class of mutants became increasingly sensitive to aminoglycosides Neomycin and Kanamycin. This included strain KG19.

tsx-2

Sensitivity to

The tsx-2 mutation caused increased sensitivity toward the antibiotic nalidixic acid.

PMID:6808961[7]

Experimental Strain: KG16


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0401

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAAAAAACATTACTGGCAGC

Primer 2:CCGAAGTTGTAACCTACTACCAG

4F3

Kohara Phage

Genobase

PMID:3038334[9]

19F6

Kohara Phage

Genobase

PMID:3038334[9]

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[3]

est. P1 cotransduction: 23% [4]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 33% [4]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11035

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11035

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946242

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001024

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1028

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001425

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ 5.0 5.1 5.2 Birch, RG et al. (1990) Stable albicidin resistance in Escherichia coli involves an altered outer-membrane nucleoside uptake system. J. Gen. Microbiol. 136 51-8 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Eeg-Larsen, N & Myhre, L (1976) Early signs of calcium and phosphorus deficiency. Bibl Nutr Dieta 129-36 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 Brunner, DP et al. (1982) Exchange of colicin receptor capacity between strains of Escherichia coli sensitive or resistant to colicin K-K235. Arch. Microbiol. 131 229-34 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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