secA:Gene: Difference between revisions
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[[:Category:Kohara_Phage:15B8|15B8]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=111 Genobase] | |||
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PMID:3038334 | |||
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est. P1 cotransduction: 18% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 18% <ref name='LIB:Nichols_linked_Tn'/> | ||
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Revision as of 21:31, 21 September 2011
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
secA |
|---|---|
| Mnemonic |
Secretory |
| Synonyms |
ECK0099, b0098, JW0096, azi, pea, prlD[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
2.33 minutes |
MG1655: 108279..110984 |
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NC_012967: 111083..113788 |
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NC_012759: 108278..110983 |
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W3110 |
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W3110: 108279..110984 |
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DH10B: 82383..85088 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
108279 |
Edman degradation |
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| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
secA207(aziR) |
azide resistant |
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secA206(aziR) |
azide resistant |
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secA214(aziR) |
azide resistant |
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secA215 |
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secA204(aziR) |
azide resistant |
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secA209(aziR) |
azide resistant |
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secA216 |
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secA201(aziR) |
azide resistant |
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secA202(aziR) |
azide resistant |
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secA203(aziR) |
azide resistant |
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secA205(aziR) |
azide resistant |
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|
secA51(ts) |
temperature sensitive |
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| edit table |
Notes
SecA mutants were first identified by Oliver and Beckwith[4] by looking for suppressors of the Lac- phenotype of a maE-lacZ fusion. The original secA alleles were ts lethals.
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0096 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCTAATCAAATTGTTAACTAA Primer 2:CCTTGCAGGCGGCCATGGCACTG | |
|
Kohara Phage |
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|
leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 39% [8] | |
|
Linked marker |
est. P1 cotransduction: 18% [8] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Schmidt, MG et al. (1988) Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli. J. Bacteriol. 170 3404-14 PubMed
- ↑ Danese, PN et al. (1995) Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. J. Bacteriol. 177 4969-73 PubMed
- ↑ Oliver, DB & Beckwith, J (1981) E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25 765-72 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).