secA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SecA

Synonyms

preprotein translocase subunit, ATPase[1], B0098[2][1], Pea[2][1], Azi[2][1], PrlD[2][1], SecA[2][1] , azi, ECK0099, JW0096, pea, prlD, b0098

Product description

SecA[2][3];

Component of Sec Protein Secretion Complex[2][3]

Translocon ATPase; targets protein precursors to the SecYE core translocon for secretion; autogenous translational repressor; ATP-dependent helicase activity on secMA mRNA; homodimeric/monomeric[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011130

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:17229438[5]

IPI: Inferred from Physical Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0005515

protein binding

PMID:17229438[5]

IPI: Inferred from Physical Interaction

EcoliWiki:secA

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01382

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000185

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011115

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:1837021[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

PMID:1837021[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006605

protein targeting

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01382

P

Seeded from EcoCyc (v14.0)

complete

GO:0006605

protein targeting

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000185

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0017038

protein import

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011115

P

Seeded from EcoCyc (v14.0)

complete

GO:0017038

protein import

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011116

P

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:17229438[5]

IPI: Inferred from Physical Interaction

EcoliWiki:secA

F

E. coli SecA is a homodimer.

complete

GO:0017038

protein import

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011130

P

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

PMID:17229438[5]

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0065002

intracellular protein transmembrane transport

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01382

P

Seeded from EcoCyc (v14.0)

complete

GO:0065002

intracellular protein transmembrane transport

PMID:6279567[7]

IMP: Inferred from Mutant Phenotype

P

secA51(Ts)

complete

GO:0065002

intracellular protein transmembrane transport

PMID:6279567[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

PMID:6279567[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006605

protein targeting

PMID:6279567[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005737

cytoplasm

PMID:1837021[6]

IDA: Inferred from Direct Assay

C

Dimeric form of SecA is cytoplasmically localized (Figure 2 & 4).

complete

GO:0005886

plasma membrane

PMID:1837021[6]

IDA: Inferred from Direct Assay

C

SecA is also strongly associated with the plasma membrane (Figure 5).

complete

GO:0043952

protein transport by the Sec complex

PMID:6279567[7]

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Sec Protein Secretion Complex

could be indirect

Protein

ycfB

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

ycaO

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

dnaK

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

gatZ

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

infC

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

minD

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

rho

PMID:15690043[8]

Experiment(s):EBI-880477

Protein

secB

PMID:15690043[8]

Experiment(s):EBI-880477, EBI-880504, EBI-886105

Protein

tufA

PMID:15690043[8]

Experiment(s):EBI-880477, EBI-889981, EBI-886105, EBI-894882

Protein

rplL

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

rplV

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

ybiU

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

aas

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

norV

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

bioB

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

motA

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

rplK

PMID:15690043[8]

Experiment(s):EBI-886105

Protein

acpP

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):18.837554

Protein

fimA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplK

PMID:19402753[9]

LCMS(ID Probability):99.4

Protein

rpsP

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

hns

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):14.030777

Protein

secB

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):17.040815

Protein

rpsJ

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

cspE

PMID:19402753[9]

LCMS(ID Probability):98.9 MALDI(Z-score):17.462556

Protein

rpmB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

kefA

PMID:19402753[9]

MALDI(Z-score):18.451503

Protein

rho

PMID:19402753[9]

MALDI(Z-score):34.875526

Protein

metK

PMID:19402753[9]

MALDI(Z-score):21.897250

Protein

mnmA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753[9]

LCMS(ID Probability):99.4 MALDI(Z-score):17.053160

Protein

minD

PMID:19402753[9]

MALDI(Z-score):30.779902

Protein

norV

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

gatZ

PMID:19402753[9]

MALDI(Z-score):21.103314

Protein

aas

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

motA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

bioB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

ybiU

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753[9]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF RARLEKGEVL
ENLIPEAFAV VREASKRVFG MRHFDVQLLG GMVLNERCIA EMRTGEGKTL TATLPAYLNA
LTGKGVHVVT VNDYLAQRDA ENNRPLFEFL GLTVGINLPG MPAPAKREAY AADITYGTNN
EYGFDYLRDN MAFSPEERVQ RKLHYALVDE VDSILIDEAR TPLIISGPAE DSSEMYKRVN
KIIPHLIRQE KEDSETFQGE GHFSVDEKSR QVNLTERGLV LIEELLVKEG IMDEGESLYS
PANIMLMHHV TAALRAHALF TRDVDYIVKD GEVIIVDEHT GRTMQGRRWS DGLHQAVEAK
EGVQIQNENQ TLASITFQNY FRLYEKLAGM TGTADTEAFE FSSIYKLDTV VVPTNRPMIR
KDLPDLVYMT EAEKIQAIIE DIKERTAKGQ PVLVGTISIE KSELVSNELT KAGIKHNVLN
AKFHANEAAI VAQAGYPAAV TIATNMAGRG TDIVLGGSWQ AEVAALENPT AEQIEKIKAD
WQVRHDAVLE AGGLHIIGTE RHESRRIDNQ LRGRSGRQGD AGSSRFYLSM EDALMRIFAS
DRVSGMMRKL GMKPGEAIEH PWVTKAIANA QRKVESRNFD IRKQLLEYDD VANDQRRAIY
SQRNELLDVS DVSETINSIR EDVFKATIDA YIPPQSLEEM WDIPGLQERL KNDFDLDLPI
AEWLDKEPEL HEETLRERIL AQSIEVYQRK EEVVGAEMMR HFEKGVMLQT LDSLWKEHLA
AMDYLRQGIH LRGYAQKDPK QEYKRESFSM FAAMLESLKY EVISTLSKVQ VRMPEEVEEL
EQQRRMEAER LAQMQQLSHQ DDDSAAAAAL AAQTGERKVG RNDPCPCGSG KKYKQCHGRL Q
Length

901

Mol. Wt

102.024 kDa

pI

5.4 (calculated)

Extinction coefficient

75,750 - 76,250 (calc based on 25 Y, 7 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

881..901

PF02810 SEC-C motif

PMID:19920124[10]

Domain

228..358

PF01043 SecA preprotein cross-linking domain

PMID:19920124[10]

Domain

617..831

PF07516 SecA Wing and Scaffold domain

PMID:19920124[10]

Domain

6..402

PF07517 SecA DEAD-like domain

PMID:19920124[10]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=secA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128091

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944821

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000343

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P10408

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10936

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10936

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944821

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000925

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0929

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Papanikolau, Y et al. (2007) Structure of dimeric SecA, the Escherichia coli preprotein translocase motor. J. Mol. Biol. 366 1545-57 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Cabelli, RJ et al. (1991) Characterization of membrane-associated and soluble states of SecA protein from wild-type and SecA51(TS) mutant strains of Escherichia coli. J. Biol. Chem. 266 24420-7 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 Oliver, DB & Beckwith, J (1982) Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli. J. Bacteriol. 150 686-91 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 9.19 9.20 9.21 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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