kefA:Gene: Difference between revisions

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Table edited by Azweifel via TableEdit
Adding section & Genetic interactions table to Gene pages.
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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:41, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

kefA

Mnemonic

potassium (K) efflux K+ efflux

Synonyms

ECK0459, b0465, JW0454, aefA, mscK[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.47 minutes 

MG1655: 485760..489122
<gbrowseImage> name=NC_000913:485760..489122 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 458603..461965
<gbrowseImage> name=NC_012967:458603..461965 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 388519..391881
<gbrowseImage> name=NC_012759:388519..391881 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 485760..489122
<gbrowseImage> name=AC_000091:485760..489122 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 425091..428453
<gbrowseImage> name=NC_010473:425091..428453 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔkefA (Keio:JW0454)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8610[3]

ΔkefA750::kan

PMID:16738554[2]

CGSC:101622



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0454

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCACTATGTTCCAGTATTACAA

Primer 2:CCCCCTACCGCTGGCGTCGGGTC

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 37% [5]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[3]

est. P1 cotransduction: 58% [5]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

zbaC3::Tn10

Linked marker

PMID:11985727[6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6255

EchoBASE (EcoliWiki Page)

EchoBASE:EB3991

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0465

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0584

EcoGene (EcoliWiki Page)

EcoGene:EG14240

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AEFA

ASAP (EcoliWikiPage)

ASAP:ABE-0001613



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ McLaggan, D et al. (2002) Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli. Mol. Microbiol. 43 521-36 PubMed

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