kefA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

kefA

Mnemonic

potassium (K) efflux K+ efflux

Synonyms

ECK0459, b0465, JW0454, aefA, mscK[1], mscK, pnuH

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.47 minutes 

MG1655: 485760..489122
<gbrowseImage> name=NC_000913:485760..489122 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 458603..461965
<gbrowseImage> name=NC_012967:458603..461965 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 388519..391881
<gbrowseImage> name=NC_012759:388519..391881 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 485760..489122
<gbrowseImage> name=NC_007779:485760..489122 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 425091..428453
<gbrowseImage> name=NC_010473:425091..428453 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

kefA(del) (Keio:JW0454)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC8610[3]

kefA750(del)::kan

PMID:16738554[2]

CGSC:101622


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0454

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCACTATGTTCCAGTATTACAA

Primer 2:CCCCCTACCGCTGGCGTCGGGTC

8C4

Kohara Phage

Genobase

PMID:3038334[5]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 37% [6]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[3]

est. P1 cotransduction: 58% [6]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

zbaC3::Tn10

Linked marker

PMID:11985727[7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (EcoliWiki Page)

GeneID:945132

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. McLaggan, D et al. (2002) Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli. Mol. Microbiol. 43 521-36 PubMed

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