pitB:Gene: Difference between revisions

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Fixing Blattner deletion strains in alleles table.
Adding section & Genetic interactions table to Gene pages.
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|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
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|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
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|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:54, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pitB

Mnemonic

Pi transport

Synonyms

ECK2981, b2987, JW2955[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

67.52 minutes 

MG1655: 3134393..3132894
<gbrowseImage> name=NC_000913:3132894..3134393 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3070458..3068959
<gbrowseImage> name=NC_012967:3068959..3070458 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3020042..3021541
<gbrowseImage> name=NC_012759:3020042..3021541 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3135027..3133528
<gbrowseImage> name=AC_000091:3133528..3135027 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3232138..3230639
<gbrowseImage> name=NC_010473:3230639..3232138 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpitB (Keio:JW2955)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10277[3]

pitB::Tn5KAN-I-SceI (FB21000)

Insertion at nt 225 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21000

contains pKD46

ΔpitB760::kan

PMID:16738554[2]

CGSC:102376



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2955

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCTAAATTTATTTGTTGGCCT

Primer 2:CCAATCAACTGCAATGCTATCCA

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[3]

est. P1 cotransduction: 33% [6]

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[3]

est. P1 cotransduction: 54% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12883

EchoBASE (EcoliWiki Page)

EchoBASE:EB2721

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2987

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3724

EcoGene (EcoliWiki Page)

EcoGene:EG12883

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PITB

ASAP (EcoliWikiPage)

ASAP:ABE-0009800



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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