parE:Gene: Difference between revisions
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Table edited by Azweifel via TableEdit |
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est. P1 cotransduction: 80% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 80% <ref name='LIB:Nichols_linked_Tn'/> | ||
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plex5BA-parEC | |||
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Plasmid Clone | |||
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*IPTG-inducible Topo IV expression plasmid | |||
*PMID:10660569 | |||
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Revision as of 18:45, 9 July 2010
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
parE |
|---|---|
| Mnemonic |
Partition |
| Synonyms |
ECK3021, b3030, JW2998, nfxD[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
68.36 minutes |
MG1655: 3173418..3171526 |
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NC_012967: 3109568..3107676 |
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NC_012759: 3058674..3060566 |
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W3110 |
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W3110: 3174052..3172160 |
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DH10B: 3271163..3269271 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
parEL445H |
L445H |
(in strain: DH161; quinolone- resistant) |
Strain variation; seeded from UniProt:P20083 | ||||
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parEC316Y |
C316Y |
Sensitivity to |
temperature sensitivity |
identical to wt TopoIV in the superhelical DNA relaxation assay at permissive temperature | |||
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2998 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACGCAAACTTATAACGCTGA Primer 2:CCAACCTCAATCTCCGCCATGTC | |
|
Linked marker |
est. P1 cotransduction: 46% [5] | ||
|
Linked marker |
est. P1 cotransduction: 80% [5] | ||
|
plex5BA-parEC |
Plasmid Clone |
| |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Nurse, P et al. (2003) Topoisomerase III can serve as the cellular decatenase in Escherichia coli. J. Biol. Chem. 278 8653-60 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Mossessova, E et al. (2000) Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative parE alleles. J. Biol. Chem. 275 4099-103 PubMed