macA:Gene: Difference between revisions

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Revision as of 21:11, 26 July 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

macA

Mnemonic

Macrolide-specific ABC-type efflux carrier

Synonyms

ECK0869, b0878, JW0862, ybjY[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

19.8 minutes 

MG1655: 918458..919573
<gbrowseImage> name=NC_000913:918458..919573 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 935941..937056
<gbrowseImage> name=NC_012967:935941..937056 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 821426..822541
<gbrowseImage> name=NC_012759:821426..822541 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 919657..920772
<gbrowseImage> name=AC_000091:919657..920772 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 972386..973501
<gbrowseImage> name=NC_010473:972386..973501 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

918458

Edman degradation

PMID:17214741[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmacA (Keio:JW0862)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8894[4]

ΔmacA779::kan

PMID:16738554[3]

CGSC:101722



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0862

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAAAGCGGAAAACCGTGAA

Primer 2:CCTTGTGCAGCTCCTGGTTTGGC

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: % [6]
Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[4]

est. P1 cotransduction: 34% [6]
Synonyms:zbj-1230::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6461

EchoBASE (EcoliWiki Page)

EchoBASE:EB3458

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0878

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1014

EcoGene (EcoliWiki Page)

EcoGene:EG13694

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z1115

ASAP (EcoliWikiPage)

ASAP:ABE-0002986



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Tikhonova, EB et al. (2007) Reconstitution of the Escherichia coli macrolide transporter: the periplasmic membrane fusion protein MacA stimulates the ATPase activity of MacB. Mol. Microbiol. 63 895-910 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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