ampC:Gene: Difference between revisions
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Revision as of 20:16, 26 July 2010
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ampC |
|---|---|
| Mnemonic |
Ampicillin |
| Synonyms |
ECK4146, b4150, JW4111, ampA[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
94.31 minutes, 94.31 minutes |
MG1655: 4376967..4375834 |
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NC_012967: 4357655..4356522 |
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NC_012759: 4314569..4315702 |
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|
W3110 |
|
W3110: 4383624..4382491 |
||
|
DH10B: 4477329..4476196 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4375837 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ampCS80D,E,G |
S80D,E,G |
Loss of activity |
seeded from UniProt:P00811 | ||||
|
ampCK83Q,T |
K83Q,T |
Lowers activity more than 1000- fold and increases protein stability |
seeded from UniProt:P00811 | ||||
|
ampCK331A |
K331A |
Lowers activity more than 1000- fold |
seeded from UniProt:P00811 | ||||
|
ampCN168D,H |
N168D,H |
Lowers activity more than 1000- fold |
seeded from UniProt:P00811 | ||||
|
ampCY166E |
Y166E |
Lowers activity more than 1000- fold |
seeded from UniProt:P00811 | ||||
|
ΔampC (Keio:JW4111) |
deletion |
deletion |
|||||
|
ampC12 |
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|
ampC11 |
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|
ampC3 |
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|
ΔampC777::kan |
| ||||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW4111 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTTCAAAACGACGCTCTGCGC Primer 2:CCtTGTAGAGCGTTAAGAATCTG | |
|
Linked marker |
est. P1 cotransduction: 50% [6] | ||
|
Linked marker |
est. P1 cotransduction: 10% [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Jaurin, B & Grundström, T (1981) ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type. Proc. Natl. Acad. Sci. U.S.A. 78 4897-901 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).