recJ:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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****************************************************************************************** -->{{Gene_nomenclature_help}} | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| | {| id="T46a9e8b4c56b5" class=" tableEdit Gene_nomenclature_table" | ||
|- | |- | ||
!align=left {{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
|| | || | ||
''recJ'' | ''recJ'' | ||
|- | |- | ||
!align=left {{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
|| | || | ||
Recombination | |||
|- | |- | ||
!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK2887, b2892, JW2860''<ref name='LIB:Riley_2006'/> | ''ECK2887, b2892, JW2860''<ref name='LIB:Riley_2006'/>, JW2860'' | ||
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****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a9e8b550bcc" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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65.4 minutes | 65.4 minutes | ||
| | | | ||
MG1655: 3036128..3034395<br /> | MG1655: 3036128..3034395<br /><gbrowseImage> | ||
name=NC_000913:3034395..3036128 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10830 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10830 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|3033395|3037128|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|3034395|3036128|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10830 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10830 EcoCyc Sequence Dump] | ||
|- | |||
| | |||
[[REL606]] | |||
| | |||
| | |||
NC_012967: 2923859..2922126<br /><gbrowseImage> | |||
name=NC_012967:2922126..2923859 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|2922126|2923859|GBrowse (REL606)}} | |||
| | |||
{{GBrowseFastaLink|REL606|NC_012967|2922126|2923859|FASTA Dump}} | |||
|- | |||
| | |||
[[BW2952]] | |||
| | |||
| | |||
NC_012759: 2921543..2923276<br /><gbrowseImage> | |||
name=NC_012759:2921543..2923276 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|2921543|2923276|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|2921543|2923276|FASTA Dump}} | |||
|- | |- | ||
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| | ||
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W3110: 3036762..3035029<br />{{ | W3110: 3036762..3035029<br /><gbrowseImage> | ||
name=NC_007779:3035029..3036762 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|W3110|NC_007779|3034029|3037762|GBrowse(W3110)}} | |||
| | |||
{{GBrowseFastaLink|W3110|NC_007779|3035029|3036762|FASTA Dump}} | |||
|- | |||
| | |||
[[DH10B]] | |||
| | |||
| | |||
DH10B: 3129998..3128265<br /><gbrowseImage> | |||
name=NC_010473:3128265..3129998 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|3124799|3133464|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|3124799|3133464|FASTA Dump}} | ||
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****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46a9e8b60cf78" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9e8b65428d" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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| | | | ||
|- | |||
| | |||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20963) | |||
| | |||
Insertion at nt 1026 in Minus orientation | |||
| | |||
| | |||
| | |||
| | |||
PMID:15262929 | |||
| | |||
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20963] | |||
| | |||
contains [[pKD46]] | |||
|- | |||
| | |||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20964) | |||
| | |||
Insertion at nt 1026 in Minus orientation | |||
| | |||
| | |||
| | |||
| | |||
PMID:15262929 | |||
| | |||
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20964] | |||
| | |||
does not contain [[pKD46]] | |||
|- | |- | ||
| | | | ||
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truncation | truncation | ||
| | | | ||
| | | | ||
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. | null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. | ||
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G to D amino acid 78 | G to D amino acid 78 | ||
| | | | ||
| | | | ||
similar to recJ284, null | similar to recJ284, null | ||
| Line 198: | Line 292: | ||
None | None | ||
| | | | ||
| | | | ||
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation | Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation | ||
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| | | | ||
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|- | |- | ||
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''recJ284:: | ''recJ284''::Tn''10'' (KD2238) | ||
| | | | ||
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| | | | ||
Resistant to | |||
| | | | ||
Mutation increases resistance to Thymineless Death (TLD). Figure 1. | |||
| | | | ||
PMID:2848620 | |||
| | | | ||
[[cgscmutation:72003|CGSC:72003]] | [[cgscmutation:72003|CGSC:72003]] | ||
| | | | ||
Parent Strain: KD2197 | |||
|- | |- | ||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
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|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9e8b710fcc" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
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Primer 2:CCAATTGGCCAGATATTGTCGAT | Primer 2:CCAATTGGCCAGATATTGTCGAT | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:5B4|5B4]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=467 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:22H4|22H4]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=468 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:5E11|5E11]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=469 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |- | ||
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Synonyms:zgd-210::Tn10, zgf-210::Tn10 | Synonyms:zgd-210::Tn10, zgf-210::Tn10 | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9e8b7aa66f" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG10830 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[EcoGene]] | |||
| | | | ||
EcoGene:EG10830 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000821 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947367 GeneID:947367] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0823 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0009492 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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==References== | ==References== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
{{RefHelp}} | |||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
[[Category:Gene List:MG1655]] | [[Category:Gene List:MG1655]] | ||
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[[Category:Genes in the Keio knockout collection]] | [[Category:Genes in the Keio knockout collection]] | ||
[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:rec genes]] | [[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | ||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:44, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
recJ |
|---|---|
| Mnemonic |
Recombination |
| Synonyms |
ECK2887, b2892, JW2860[1], JW2860 |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
65.4 minutes |
MG1655: 3036128..3034395 |
||
|
NC_012967: 2923859..2922126 |
||||
|
NC_012759: 2921543..2923276 |
||||
|
W3110 |
|
W3110: 3036762..3035029 |
||
|
DH10B: 3129998..3128265 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔrecJ (Keio:JW2860) |
deletion |
deletion |
|||||
|
recJ::Tn5KAN-I-SceI (FB20963) |
Insertion at nt 1026 in Minus orientation |
contains pKD46 | |||||
|
recJ::Tn5KAN-I-SceI (FB20964) |
Insertion at nt 1026 in Minus orientation |
does not contain pKD46 | |||||
|
recJ284::Tn10 |
Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon |
truncation |
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC. |
CGSC |
|||
|
recJ77 |
G to A nucleotide 281 |
G to D amino acid 78 |
similar to recJ284, null |
||||
|
recJ153 recJ154 |
GTG to GTA at start codon |
None |
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation |
||||
|
recJ153 |
|||||||
|
recJ284::Tn10 (KD2238) |
Resistant to |
Mutation increases resistance to Thymineless Death (TLD). Figure 1. |
Parent Strain: KD2197 | ||||
|
ΔrecJ743::kan |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2860 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAACAACAGATACAACTTCG Primer 2:CCAATTGGCCAGATATTGTCGAT | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: % [12] | ||
|
Linked marker |
est. P1 cotransduction: 9% [12] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 5.0 5.1 5.2 Lovett, ST & Clark, AJ (1984) Genetic analysis of the recJ gene of Escherichia coli K-12. J. Bacteriol. 157 190-6 PubMed
- ↑ Gillen, JR et al. (1981) Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J. Bacteriol. 145 521-32 PubMed
- ↑ Horii, Z & Clark, AJ (1973) Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J. Mol. Biol. 80 327-44 PubMed
- ↑ Haggerty, TJ & Lovett, ST (1997) IF3-mediated suppression of a GUA initiation codon mutation in the recJ gene of Escherichia coli. J. Bacteriol. 179 6705-13 PubMed
- ↑ Nakayama, K et al. (1988) Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway. Can. J. Microbiol. 34 905-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 11.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).