eno:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
enolase<ref name='LIB:Riley_2006'/>, B2779<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Eno<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''enolase<ref name='LIB:Riley_2006'/>, B2779<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Eno<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2773, JW2750, b2779''
|-
|-
!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
Line 52: Line 52:
Component of [[:Category:Complex:enolase|enolase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; [[:Category:Complex:degradosome|degradosome]]<ref name='LIB:EcoCyc11.1'/>
Component of [[:Category:Complex:enolase|enolase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; [[:Category:Complex:degradosome|degradosome]]<ref name='LIB:EcoCyc11.1'/>


Enolase; phosphoprotein; component of RNA degradosome<ref name='LIB:EcoGene'/>
|-
|-
!align=left {{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
||
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11]<ref name='LIB:Riley_2006'/>


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{{Product_nomenclature_help}}
 
<protect><!--Nomenclature_notes--></protect>
<protect><!--Nomenclature_notes--></protect>
===Notes===
===Notes===
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{{BacktoTop}}
{{BacktoTop}}
<protect>
<protect>
<big>'''Gene Ontology'''</big><br />{{Product_GO_help}}<!--box uid=421aa90e079fa326b6494f812ad13e79.25894.C46aabd48d3832-->
<big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.25894.C46aabd48d3832-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46aabd48d3832"  class=" tableEdit GO_table_product dataTable" 
|- {{table_heading_style}}
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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
 
|
GO:0004634
|
phosphopyruvate hydratase activity
|
PMID:4942326
|
IDA: Inferred from Direct Assay
|
 
|
F
|
 
|
complete
|-
|
 
|
GO:0000015
|
phosphopyruvate hydratase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000941
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000015
|
phosphopyruvate hydratase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020809
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000015
|
phosphopyruvate hydratase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020810
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000015
|
phosphopyruvate hydratase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020811
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0046872_!_metal_ion_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0046872_!_metal_ion_binding GO:0046872]
GO:0000287
|
|
metal ion binding
magnesium ion binding
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
GOA:hamap<br />GO_REF:0000020
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/keywords/KW-0479 SP_KW:KW-0479]
HAMAP:MF_00318
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 102: Line 198:


|
|
[[Category:GO:0016829_!_lyase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016829_!_lyase_activity GO:0016829]
GO:0000287
|
|
lyase activity
magnesium ion binding
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000941
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000287
|
magnesium ion binding
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020809
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000287
|
magnesium ion binding
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/keywords/KW-0456 SP_KW:KW-0456]
InterPro:IPR020810
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 121: Line 255:


|
|
[[Category:GO:0006096_!_glycolysis]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006096_!_glycolysis GO:0006096]
GO:0000287
|
|
glycolysis
magnesium ion binding
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]<br />[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]<br />[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR000941 InterPro:IPR000941]<br />[http://www.uniprot.org/keywords/KW-0324 SP_KW:KW-0324]<br />[http://ca.expasy.org/unirule/MF_00318 HAMAP:MF_00318]
InterPro:IPR020811
|
|
P
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 140: Line 274:


|
|
[[Category:GO:0005856_!_cytoskeleton]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005856_!_cytoskeleton GO:0005856]
GO:0000287
|
|
cytoskeleton
magnesium ion binding
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/keywords/KW-0206 SP_KW:KW-0206]
SP_KW:KW-0460
|
|
C
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000287
|
magnesium ion binding
|
PMID:16516921
|
IDA: Inferred from Direct Assay
|
 
|
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 159: Line 312:


|
|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
GO:0004634
|
|
cytoplasm
phosphopyruvate hydratase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
GOA:hamap<br />GO_REF:0000020
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/keywords/KW-0963 SP_KW:KW-0963]
HAMAP:MF_00318
|
|
C
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 178: Line 331:


|
|
[[Category:GO:0004634_!_phosphopyruvate_hydratase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004634_!_phosphopyruvate_hydratase_activity GO:0004634]
GO:0004634
|
|
phosphopyruvate hydratase activity
phosphopyruvate hydratase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]<br />[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000003 GO_REF:0000003]<br />[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR000941 InterPro:IPR000941]<br />[http://www.expasy.org/cgi-bin/nicezyme.pl?4.2.1.11 EC:4.2.1.11]<br />[http://ca.expasy.org/unirule/MF_00318 HAMAP:MF_00318]
InterPro:IPR000941
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 197: Line 350:


|
|
[[Category:GO:0000287_!_magnesium_ion_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0000287_!_magnesium_ion_binding GO:0000287]
GO:0004634
|
|
magnesium ion binding
phosphopyruvate hydratase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]<br />[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/keywords/KW-0460 SP_KW:KW-0460]<br />[http://ca.expasy.org/unirule/MF_00318 HAMAP:MF_00318]
InterPro:IPR020809
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 216: Line 369:


|
|
[[Category:GO:0000015_!_phosphopyruvate_hydratase_complex]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0000015_!_phosphopyruvate_hydratase_complex GO:0000015]
GO:0005515
|
protein binding
|
PMID:16516921
|
IPI: Inferred from Physical Interaction
|
|
phosphopyruvate hydratase complex
EcoliWiki:eno
 
 
|
F
|
Enolase is homodimeric (Figure 1).
|
complete
|-
|
 
|
GO:0004634
|
phosphopyruvate hydratase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR000941 InterPro:IPR000941]
InterPro:IPR020810
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005515
|
protein binding
|
PMID:16516921
|
IPI: Inferred from Physical Interaction
|
EcoliWiki:rne
 
 
|
|
C
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete  
complete  
Line 235: Line 430:


|
|
[[Category:GO:0004634_!_phosphopyruvate_hydratase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004634_!_phosphopyruvate_hydratase_activity GO:0004634]
GO:0004634
|
|
phosphopyruvate hydratase activity
phosphopyruvate hydratase activity
|[[PMID:4942326]]<ref name=PMID:4942326/>
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020811
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0000287
|
magnesium ion binding
|
PMID:16516921
|
|
IDA: Inferred from Direct Assay
IDA: Inferred from Direct Assay
Line 246: Line 461:
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Each monomer binds and coordinates 1 MG<sup>2+</sup> cation (Figure 3).
|
complete
|-
|
 
|
GO:0004634
|
phosphopyruvate hydratase activity
|
GOA:spec<br />GO_REF:0000003
|
IEA: Inferred from Electronic Annotation
|
EC:4.2.1.11
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
PMID:410789
|
IMP: Inferred from Mutant Phenotype
|
 
|
P
|
 
|
|
complete  
complete  
Line 253: Line 506:


|
|
[[Category:GO:0005515_!_protein_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005515_!_protein_binding GO:0005515]
GO:0005515
|
|
protein binding
protein binding
|[[PMID:15236960]]<ref name=PMID:15236960/>
|
PMID:16516921
|
|
IPI: Inferred from Physical Interaction
IPI: Inferred from Physical Interaction
|
|
[http://www.uniprot.org/uniprot/P21513 UniProtKB:P21513]
 
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
Missing: with/from
|-
|
 
|
GO:0005515
|
protein binding
|
PMID:16516921
|
IPI: Inferred from Physical Interaction
|
EcoliWiki:eno
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005576
|
extracellular region
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0964
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005576
|
extracellular region
|
GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_SL:SL-0243
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 271: Line 582:


|
|
[[Category:GO:0005624_!_membrane_fraction]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005624_!_membrane_fraction GO:0005624]
GO:0005829
|
|
membrane fraction
cytosol
|[[PMID:16858726]]<ref name=PMID:16858726/>
|
PMID:16858726
|
|
IDA: Inferred from Direct Assay
IDA: Inferred from Direct Assay
Line 282: Line 594:
C
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 289: Line 601:


|
|
[[Category:GO:0042802_!_identical_protein_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042802_!_identical_protein_binding GO:0042802]
GO:0005856
|
cytoskeleton
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
identical protein binding
IEA: Inferred from Electronic Annotation
|[[PMID:15236960]]<ref name=PMID:15236960/>
|
|
IPI: Inferred from Physical Interaction
SP_KW:KW-0206
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005856
|
cytoskeleton
|
GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_SL:SL-0090
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
GOA:hamap<br />GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00318
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000941
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020809
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR020810
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0006096
|
glycolysis
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
|
[http://www.uniprot.org/uniprot/P0A6P9 UniProtKB:P0A6P9]
InterPro:IPR020811
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
[[Category:GO:0009061_!_anaerobic_respiration]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009061_!_anaerobic_respiration GO:0009061]
GO:0006096
|
|
anaerobic respiration
glycolysis
|
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0324
|
|
P  
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|
 
|
|
[[Category:GO:0006094_!_gluconeogenesis]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006094_!_gluconeogenesis GO:0006094]
GO:0009986
|
|
gluconeogenesis
cell surface
|
|
GOA:hamap<br />GO_REF:0000020
|
|
IEA: Inferred from Electronic Annotation
|
|
HAMAP:MF_00318
|
|
P
C
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|
 
|
GO:0009986
|
cell surface
|
GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_SL:SL-0310
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0016020
|
|
[[Category:GO:0004634_!_phosphopyruvate_hydratase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004634_!_phosphopyruvate_hydratase_activity GO:0004634]
membrane
|
|
phosphopyruvate hydratase activity
PMID:16858726
|[[PMID:4942326]]<ref name=PMID:4942326/>
|
|
IDA: Inferred from Direct Assay
IDA: Inferred from Direct Assay
Line 354: Line 801:


|
|
F
C
|
|
purification, assay according to ref 5, which is not in Pubmed
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  


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*  
*  
****************************************************************************************** -->{{Product_interactions_help}}
****************************************************************************************** -->{{Product_interactions_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M46aabd66df967"  class=" tableEdit Product_interactions_table dataTable"   
|- {{table heading style}}
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
Line 380: Line 827:
Protein
Protein
|
|
[http://www.uniprot.org/uniprot/Subuni Subuni]ts of [[:Category:Complex:enolase|enolase]]  
Subunits of [[:Category:Complex:enolase|enolase]]  
|
|
could be indirect  
could be indirect  
Line 391: Line 838:
Protein
Protein
|
|
[[aroF:Gene_Product(s)|aroF]]<br />
aroF<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
rplI<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
yfeX<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
rplW<br />
|
|


|[[PMID:16606699]]<ref name=PMID:16606699/>
|
|
Experiment(s):[http://www.ebi.ac.uk/iintact/search/do/search?searchString=EBI-1143906 EBI-1143906]<br />  
PMID:16606699
|
Experiment(s):EBI-1143906<br />  
|-  
|-  
|
|
Protein
Protein
|
|
[[rplI:Gene_Product(s)|rplI]]<br />
nadE<br />
|
|


|[[PMID:16606699]]<ref name=PMID:16606699/>
|
|
Experiment(s):[http://www.ebi.ac.uk/iintact/search/do/search?searchString=EBI-1143906 EBI-1143906]<br />  
PMID:16606699
|
Experiment(s):EBI-1143906<br />  
|-  
|-  
|
|
Protein
Protein
|
|
[http://www.uniprot.org/uniprot/Subuni Subuni]ts of [[:Category:Complex:degradosome|degradosome]]
rdgC<br />
|
 
|
PMID:16606699
|
|
could be indirect
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
ybdN<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
yqjK<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
racR<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
nanE<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1143906<br />
|-
|
Protein
|
dnaK<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-880413, EBI-885840<br />
|-
|
Protein
|
fabZ<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-880413<br />
|-
|
Protein
|
gltX<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-880413<br />
|-
|
Protein
|
pnp<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-880413, EBI-883913, EBI-891089, EBI-885840<br />
|-
|
Protein
|
yniC<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-880413<br />
|-
|
Protein
|
pinR<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-885840<br />
|-
|
Protein
|
rpsE<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-885840<br />
|-
|
Protein
|
pinR
|
|


|
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-  
|-  
|
|
Protein
Protein
|
|
[[yfeX:Gene_Product(s)|yfeX]]<br />
Subunits of [[:Category:Complex:degradosome|degradosome]]  
|
could be indirect
|
|


|[[PMID:16606699]]<ref name=PMID:16606699/>
|
|
Experiment(s):[http://www.ebi.ac.uk/iintact/search/do/search?searchString=EBI-1143906 EBI-1143906]<br />


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{{Product_interactions_help}}
 
<!--Function_notes--></protect>
<!--Function_notes--></protect>
===Notes===
===Notes===
Line 446: Line 1,061:
*  
*  
****************************************************************************************** -->{{Product_localization_help}}
****************************************************************************************** -->{{Product_localization_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46aabd68a7ab7"  class=" tableEdit Product_localization_table"   
|- {{table heading style}}
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-  
|-  
|
|
Line 457: Line 1,072:
   
   
|
|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>   
From EcoCyc<ref name='LIB:EcoCyc11.1'/>  
|
|-
|
Cytoplasm
|
 
|
PMID:9298646, PMID:8610017, PMID:9600841, PMID:9740056, PMID:9732527, PMID:12035760
|
[http://www.york.ac.uk/res/thomas/Echolocation.cfm?EB_SubLocID=EB0254 EchoLocation:eno]
|
   


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{{Product_localization_help}}
 
<protect><!--Localization_notes--></protect>
<protect><!--Localization_notes--></protect>


Line 480: Line 1,108:
*  
*  
****************************************************************************************** -->{{Product_physical_properties_help}}
****************************************************************************************** -->{{Product_physical_properties_help}}
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
{|   id="C46aabd6a477d5" class=" tableEdit Product_phys_prop_table"
 
|-
!align=left {{table heading style}} |Name
||


|-
|-
Line 494: Line 1,126:
DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA
DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA
PYNGRKEIKG QA</pre>  
PYNGRKEIKG QA</pre>  
|-
!align=left {{table heading style}} |Length
||
432
|-
|-
!align=left {{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
||
||
45.65 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
45.656 kDa
|-
|-
!align=left {{table heading style}} |pI
!align=left {{table heading style}} |pI
||
||
5.64<ref name='LIB:EcoCyc10.6'/>
5.3 (calculated)
|-
|-
!align=left {{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
21890 - 22015 (calc based on 11 Y, 1 W, and 1 C residues)  
21,890 - 22,015 (calc based on 11 Y, 1 W, and 1 C residues)


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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span>
 
<!--box uid=421aa90e079fa326b6494f812ad13e79.25894.K46aabd6ab740f-->
<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
<tr align="left" valign="top">
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.25894.K46aabd6ab740f-->
<!--
<!--
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*  
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****************************************************************************************** -->{{Product_domains_motifs_help}}
****************************************************************************************** -->{{Product_domains_motifs_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aabd6ab740f"  class=" tableEdit Product_motif_table dataTable"   
|- {{table heading style}}
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|
Initiator Methionine
|
1
|
Removed
|
[http://www.uniprot.org/uniprot/P0A6P9#section_features UniProt:P0A6P9]
|
|-
|
Modification Site
|
375
|
phosphorylation site at T375
|
[[PHOSIDA#p-values|probability less than 75%]]
|
PMID:17938405
|-
|
Modification Site
|
379
|
phosphorylation site at T379
|
[[PHOSIDA#p-values|probability less than 75%]]
|
PMID:17938405
|-
|
Modification Site
|
372
|
phosphorylation site at S372
|
[[PHOSIDA#p-values|probability less than 75%]]
|
PMID:17938405
|-
|
Domain
|
143..431
|
[http://pfam.janelia.org/family/PF00113 PF00113  Enolase, C-terminal TIM barrel domain]
|
 
|
PMID:19920124
|-
|
Domain
|
3..134
|
[http://pfam.janelia.org/family/PF03952 PF03952  Enolase, N-terminal domain]
|


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PMID:19920124
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25894.K46aabd6ab740f&page=25894&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table]</span> || || || ||
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{{Product_domains_motifs_help}}
<td>
<!-- motifs column 2-->
<motif_map/>
</td>
</tr>
</table>


<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
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*  
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****************************************************************************************** -->{{Product_structure_help}}
****************************************************************************************** -->{{Product_structure_help}}
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{|   id="G46a85415ed966" class=" tableEdit Product_structure_table"


|-
|-
!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
||
||
<beststructure>eno
<beststructure>
562</beststructure>
gene=eno
 
taxon=562,83333
</beststructure>
|-
|-
!align=left {{table heading style}} |Models
!align=left {{table heading style}} |Models
||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=eno&sub=keywordSearc EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0A6P9 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=eno&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A6P9 PSI-PRED (P0A6P9)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A6P9 Genomes TO Protein (P0A6P9)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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{{Product_structure_help}}
 
<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
<protect><!--          ***** PLEASE DO NOT EDIT BELOW THIS LINE *****        -->
</td><tr><table><!--Structure_notes--></protect>
===Notes===
===Notes===


Line 576: Line 1,305:
*  
*  
****************************************************************************************** -->{{Product_resources_help}}
****************************************************************************************** -->{{Product_resources_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N46aabd6b81773"  class=" tableEdit Product_resource_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference


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{{Product_resources_help}}
 
<protect><!--Resources_notes--></protect>
<protect><!--Resources_notes--></protect>
===Notes===
===Notes===
Line 598: Line 1,327:
*  
*  
****************************************************************************************** -->{{Gene_accessions_help}}
****************************************************************************************** -->{{Gene_accessions_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="E46aabd6be03f3"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=ENOLASE-MONOMER EcoCyc:ENOLASE-MONOMER]
[http://www.ncbi.nlm.nih.gov/protein/16130686 GI:16130686]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_417259.1 GeneID:945032]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0009110
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0A6P9 UniProtKB/Swiss-Prot:P0A6P9]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG10258
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoGene]]
|
|
[[uniprot:P0A6P9|UniProt:P0A6P9]]
EcoGene:EG10258
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://pfam.sanger.ac.uk/family?acc=PF03952 PFAM:PF03952]
[http://www.ncbi.nlm.nih.gov/gene?term=945032 GeneID:945032]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417259 RefSeq:NP_417259]
RegulonDB:ECK120000252
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A6P9 ModBase:P0A6P9]
EchoBASE:EB0254
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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===Categories===
===Categories===
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[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006096_!_glycolysis]][[Category:GO:0009061_!_anaerobic respiration]][[Category:GO:0006094_!_gluconeogenesis]][[Category:Complex:enolase]][[Category:Complex:degradosome]][[Category:Location:cytoplasm]]
[[Category:RefGenome_Annotated_Gene]]
[[Category:Pathway:Glycolysis]]
[[Category: GO Annotation by EcoliWiki Team]]

Latest revision as of 04:21, 19 May 2019

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Eno

Synonyms

enolase[1], B2779[2][1], Eno[2][1] , ECK2773, JW2750, b2779

Product description

Eno[2][3];

Component of enolase[2][3]; degradosome[3]

Enolase; phosphoprotein; component of RNA degradosome[4]

EC number (for enzymes)


Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004634

phosphopyruvate hydratase activity

PMID:4942326[5]

IDA: Inferred from Direct Assay

F

complete

GO:0000015

phosphopyruvate hydratase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000941

C

Seeded from EcoCyc (v14.0)

complete

GO:0000015

phosphopyruvate hydratase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020809

C

Seeded from EcoCyc (v14.0)

complete

GO:0000015

phosphopyruvate hydratase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020810

C

Seeded from EcoCyc (v14.0)

complete

GO:0000015

phosphopyruvate hydratase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020811

C

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00318

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000941

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020809

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020810

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020811

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

PMID:16516921[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00318

F

Seeded from EcoCyc (v14.0)

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000941

F

Seeded from EcoCyc (v14.0)

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020809

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16516921[6]

IPI: Inferred from Physical Interaction

EcoliWiki:eno


F

Enolase is homodimeric (Figure 1).

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020810

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16516921[6]

IPI: Inferred from Physical Interaction

EcoliWiki:rne


F

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020811

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

PMID:16516921[6]

IDA: Inferred from Direct Assay

F

Each monomer binds and coordinates 1 MG2+ cation (Figure 3).

complete

GO:0004634

phosphopyruvate hydratase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.1.11

F

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

PMID:410789[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005515

protein binding

PMID:16516921[6]

IPI: Inferred from Physical Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0005515

protein binding

PMID:16516921[6]

IPI: Inferred from Physical Interaction

EcoliWiki:eno

F

Seeded from EcoCyc (v14.0)

complete

GO:0005576

extracellular region

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0964

C

Seeded from EcoCyc (v14.0)

complete

GO:0005576

extracellular region

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0243

C

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[8]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005856

cytoskeleton

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0206

C

Seeded from EcoCyc (v14.0)

complete

GO:0005856

cytoskeleton

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0090

C

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00318

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000941

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020809

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020810

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020811

P

Seeded from EcoCyc (v14.0)

complete

GO:0006096

glycolysis

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0324

P

Seeded from EcoCyc (v14.0)

complete

GO:0009986

cell surface

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00318

C

Seeded from EcoCyc (v14.0)

complete

GO:0009986

cell surface

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0310

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726[8]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of enolase

could be indirect

Protein

aroF

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

rplI

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

yfeX

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

rplW

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

nadE

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

rdgC

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

ybdN

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

yqjK

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

racR

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

nanE

PMID:16606699[9]

Experiment(s):EBI-1143906

Protein

dnaK

PMID:15690043[10]

Experiment(s):EBI-880413, EBI-885840

Protein

fabZ

PMID:15690043[10]

Experiment(s):EBI-880413

Protein

gltX

PMID:15690043[10]

Experiment(s):EBI-880413

Protein

pnp

PMID:15690043[10]

Experiment(s):EBI-880413, EBI-883913, EBI-891089, EBI-885840

Protein

yniC

PMID:15690043[10]

Experiment(s):EBI-880413

Protein

pinR

PMID:15690043[10]

Experiment(s):EBI-885840

Protein

rpsE

PMID:15690043[10]

Experiment(s):EBI-885840

Protein

pinR

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

Subunits of degradosome

could be indirect



Notes

IMP for enolase activity probably in [12] (based on abstract. need to get paper and read).

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]

Cytoplasm

PMID:9298646[13], PMID:8610017[14], PMID:9600841[15], PMID:9740056[16], PMID:9732527[17], PMID:12035760[18]

EchoLocation:eno



Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSKIVKIIGR EIIDSRGNPT VEAEVHLEGG FVGMAAAPSG ASTGSREALE LRDGDKSRFL
GKGVTKAVAA VNGPIAQALI GKDAKDQAGI DKIMIDLDGT ENKSKFGANA ILAVSLANAK
AAAAAKGMPL YEHIAELNGT PGKYSMPVPM MNIINGGEHA DNNVDIQEFM IQPVGAKTVK
EAIRMGSEVF HHLAKVLKAK GMNTAVGDEG GYAPNLGSNA EALAVIAEAV KAAGYELGKD
ITLAMDCAAS EFYKDGKYVL AGEGNKAFTS EEFTHFLEEL TKQYPIVSIE DGLDESDWDG
FAYQTKVLGD KIQLVGDDLF VTNTKILKEG IEKGIANSIL IKFNQIGSLT ETLAAIKMAK
DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA
PYNGRKEIKG QA
Length

432

Mol. Wt

45.656 kDa

pI

5.3 (calculated)

Extinction coefficient

21,890 - 22,015 (calc based on 11 Y, 1 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6P9

Modification Site

375

phosphorylation site at T375

probability less than 75%

PMID:17938405[19]

Modification Site

379

phosphorylation site at T379

probability less than 75%

PMID:17938405[19]

Modification Site

372

phosphorylation site at S372

probability less than 75%

PMID:17938405[19]

Domain

143..431

PF00113 Enolase, C-terminal TIM barrel domain

PMID:19920124[20]

Domain

3..134

PF03952 Enolase, N-terminal domain

PMID:19920124[20]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=eno taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130686

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945032

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009110

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6P9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10258

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10258

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945032

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000252

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0254

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Spring, TG & Wold, F (1971) The purification and characterization of Escherichia coli enolase. J. Biol. Chem. 246 6797-802 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Chandran, V & Luisi, BF (2006) Recognition of enolase in the Escherichia coli RNA degradosome. J. Mol. Biol. 358 8-15 PubMed
  7. ↑ Irani, MH & Maitra, PK (1977) Properties of Escherichia coli mutants deficient in enzymes of glycolysis. J. Bacteriol. 132 398-410 PubMed
  8. ↑ 8.0 8.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 10.5 10.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  11. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  12. ↑ Irani, MH & Maitra, PK (1976) Glyceraldehyde 3-p dehydrogenase, glycerate 3-P kinase and enolase mutants of Escherichia coli: genetic studies. Mol. Gen. Genet. 145 65-71 PubMed
  13. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  14. ↑ Py, B et al. (1996) A DEAD-box RNA helicase in the Escherichia coli RNA degradosome. Nature 381 169-72 PubMed
  15. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  16. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  17. ↑ Rompf, A et al. (1998) Changes in protein synthesis as a consequence of heme depletion in Escherichia coli. Curr. Microbiol. 37 226-30 PubMed
  18. ↑ Carpousis, AJ (2002) The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes. Biochem. Soc. Trans. 30 150-5 PubMed
  19. ↑ 19.0 19.1 19.2 Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
  20. ↑ 20.0 20.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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