PMID:16516921
| Citation |
Chandran, V and Luisi, BF (2006) Recognition of enolase in the Escherichia coli RNA degradosome. J. Mol. Biol. 358:8-15 |
|---|---|
| Abstract |
In Escherichia coli, the glycolytic enzyme enolase is a component of the RNA degradosome, which is an RNase E mediated assembly involved in RNA processing and transcript turnover. The recruitment of enolase by the RNA degradosome has been implicated in the turnover of certain transcripts, and it is mediated by a small segment of roughly a dozen residues that lie within a natively unstructured sub-domain of RNase E. Here, we present the crystal structure of enolase in complex with its recognition site from RNase E at 1.6A resolution. A single molecule of the RNase E peptide binds asymmetrically in a conserved cleft at the interface of the enolase dimer. The recognition site is well conserved in RNase E homologues in a subfamily of the gamma-proteobacteria, including enzymes from pathogens such as Yersinia pestis, Vibrio cholera and Salmonella sp. We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions. |
| Links |
PubMed Online version:10.1016/j.jmb.2006.02.012 |
| Keywords |
Amino Acid Sequence; Binding Sites; Crystallography, X-Ray; Endoribonucleases/chemistry; Endoribonucleases/metabolism; Escherichia coli/cytology; Escherichia coli/enzymology; Models, Molecular; Molecular Sequence Data; Multienzyme Complexes/metabolism; Phosphopyruvate Hydratase/chemistry; Phosphopyruvate Hydratase/metabolism; Polyribonucleotide Nucleotidyltransferase/metabolism; Protein Binding; RNA Helicases/metabolism; RNA, Bacterial/metabolism; Static Electricity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
