mscL:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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****************************************************************************************** -->{{Gene_nomenclature_help}}
{|{{Prettytable}}
{|   id="J46a9f23915d43"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''mscL''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''mscL''
Mechanosensitive channel
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>, stkA, yhdC''


|-
|- class="tableEdit_footer"
!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
|
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}} edit table]
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<!--Nomenclature_notes--></protect>
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===Notes===
===Notes===
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****************************************************************************************** -->{{Gene_location_help}}
{|class = 'sortable' {{Prettytable}} id='144591'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="S46a9f239962db"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
Line 72: Line 76:
74.06 minutes&nbsp;
74.06 minutes&nbsp;
|
|
MG1655: 3436046..3436456<br />{{GBrowseFigureByCoord|3435046|3437456|ecoli|MG1655}}
MG1655: 3436046..3436456<br /><gbrowseImage>
name=NC_000913:3436046..3436456
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:3435046..3437456 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|3435046|3437456|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:3436046..3436456&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|3436046|3436456|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 3365929..3366339<br /><gbrowseImage>
name=NC_012967:3365929..3366339
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|3365929|3366339|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3365929|3366339|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 3323203..3323613<br /><gbrowseImage>
name=NC_012759:3323203..3323613
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|3323203|3323613|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3323203|3323613|FASTA Dump}}
|-  
|
|
W3110
W3110
Line 86: Line 127:
&nbsp;
&nbsp;
|
|
W3110: 4202392..4201982<br />{{GBrowseFigureByCoord|4200982|4203392|ecoli_W3110|W3110}}  
W3110: 4202392..4201982<br /><gbrowseImage>
name=NC_007779:4201982..4202392
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|4200982|4203392|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4201982|4202392|FASTA Dump}}  
|-
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:4200982..4203392 GBrowse(W3110)]  
[[DH10B]]
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:4201982..4202392&plugin_action=Go FASTA Dump]
 
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DH10B: 3533791..3534201<br /><gbrowseImage>
name=NC_010473:3533791..3534201
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|3532971|3535021|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3532971|3535021|FASTA Dump}}
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.S46a9f239962db&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table]</span> || || || ||
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<!--Location_notes--></protect>
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-class='sortbottom'
|-
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|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3436046
|
Edman degradation
|
PMID:7511799<br />
|
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.T46a9f23a56149&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table]</span> || || || || ||
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====Notes====
====Notes====
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****************************************************************************************** -->{{Gene_alleles_help}}
{|class = 'sortable' {{Prettytable}} id='144593'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9f23a9ebac"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|-  
|
|
&Delta;mscL (Keio:JW3252)
&Delta;mscL (Keio:JW3252)
Line 145: Line 222:


|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/>
|
|-
|
''&#916;mscL727::kan''
|
 
|
 
|
 
|
 
|
PMID:16738554
|
[[cgscmutation:101954|CGSC:101954]]
|
|-
|
mscL-K31C
|
-->TGC
|
K31C
|
 
|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See Table 1
|-
|
''mscL-K31R''
|
-->AGG
|
K31R
|
 
|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-K31E''
|
-->GAG
|
K31E
|
Sensitivity to
|
Activation pressure lowered by 18.3 mmHg
|
PMID:9635742
|
Parental:AW405
|
See table 4
|-
|
''mscL-Q56P''
|
-->CCT
|
Q56P
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental:AW405
|
see table 1
|-
|
''mscL-Q56C''
|
-->TGC
|
Q56C
|
Conductance
|
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56F''
|
-->TTT
|
Q56F
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
|
Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
|
-->TAC
|
Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56L''
|
-->TTA
|
Q56L
|
 
|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56R''
|
-->CGT
|
Q56R
|
Sensitivity to
|
Activation pressure lowered by 17.9 mmHg
|
PMID:9635742
|
Parental: AW405
|
See table 4
|-
|
''mscLΔ2-4''
|
Deletion
|
 
|
 
|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/> 
Parental: AW405
|
|
See table 1
|-
|
''mscL-ΔQ56''
|
Deletion
|
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ110-136''
|
Deletion
|
|
|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
   
   
|-class='sortbottom'
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|
''mscL-Q56H''
|
-->CAT
|
Q56H
|
Sensitivity to
|
Increased sensitivity to pressure at pH 5 (activation pressure lowered by 18.6 mmHg) and increased open channel time at pH 7.5.
|
PMID:8876191
PMID:9635742
|
Parental: AW405
|
See table 1 and table 4
|-
|
''mscL-K31D''
|
-->GAT
|
K31D
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|
 
|
See figure 1
|-
|
''mscL-K31E''
|
 
|
K31E
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|
 
|
See figure 1
|-
|
''mscLΔ104-136''
|
Deletion
|
 
|
Conductance
|
Failed to exhibit functional channel
|
PMID:9141355
|
 
|
See table 2
|-
|
''mscLΔEV''
|
Internal deletion (Δ41-61)
|
 
|
Conductance
|
Failed to show functional channels
|
PMID:9141355
|
 
|
See table 2
|-
|
''mscLNBE''
|
Deletion (Δ1-8)
|
 
|
Conductance
|
Decreased conductance
|
PMID:9141355
|
 
|
See table 2
|-
|
''mscL-G22R''
|
 
|
G22R
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22K''
|
 
|
G22K
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22D''
|
 
|
G22D
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22E''
|
 
|
G22D
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22S''
|
 
|
G22S
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1. 
|-
|
''mscL-G22Y''
|
 
|
G22Y
|
Growth Phenotype
|
Growth decreased
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1
|-
|
''mscL-G22Q''
|
 
|
G22Q
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
mscL-G22T''
|
 
|
G22T
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22V''
|
 
|
G22V
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1
|-
|
''mscL-G22L''
|
 
|
G22L
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22W''
|
 
|
G22W
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22F''
|
 
|
G22F
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G11I''
|
 
|
G22I
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22M''
|
 
|
G22M
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22C''
|
 
|
G22C
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22P''
|
 
|
G22P
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.U46a9f23a9ebac&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || ||
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{{Gene_alleles_help}}
 
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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J4e04bfa3717ad"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
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===Notes===


==Genetic Resources==
==Genetic Resources==
Line 164: Line 879:
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
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JW3252
JW3252
Line 179: Line 894:
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 186: Line 901:


Primer 2:CCAGAGCGGTTATTCTGCTCTTT  
Primer 2:CCAGAGCGGTTATTCTGCTCTTT  
|-
|-  
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[[smg:Gene|smg-3082::Tn10]]
[[smg:Gene|smg-3082::Tn10]]
Line 194: Line 909:
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10  
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10  
|-
|-  
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[[yhfT:Gene|zhf-3084::Tn10]]
[[yhfT:Gene|zhf-3084::Tn10]]
Line 205: Line 919:
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
Synonyms:zhe-3084::Tn10
 


CAG18456 also carries cysG0 (CGSC).
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===Notes===
===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11180 EcoCyc:EG11180] 
EcoCyc:EG11180
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1167 EchoBASE:EB1167] 
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b3291 EcoliGenExpDB:b3291] 
EcoGene:EG11180
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
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[http://BioCyc.org/ECOL199310/new-image?object=C4051 ECOL199310CYC:C4051] 
RegulonDB:ECK120001162
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11180 EcoGene:EG11180]
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
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Escherichia coli str. K-12 substr. MG1655
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
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[http://BioCyc.org/ECOO157/new-image?object=MSCL ECOO157CYC:MSCL] 
EchoBASE:EB1167
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/35417 CGSC:35417] 
ASAP:ABE-0010790
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
===Notes===
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<references/>
<references />
__NOTOC__
__NOTOC__
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===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:msc genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 19:26, 13 November 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mscL

Mnemonic

Mechanosensitive channel

Synonyms

ECK3277, b3291, JW3252, yhdC[1], stkA, yhdC

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.06 minutes 

MG1655: 3436046..3436456
<gbrowseImage> name=NC_000913:3436046..3436456 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3365929..3366339
<gbrowseImage> name=NC_012967:3365929..3366339 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3323203..3323613
<gbrowseImage> name=NC_012759:3323203..3323613 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4202392..4201982
<gbrowseImage> name=NC_007779:4201982..4202392 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3533791..3534201
<gbrowseImage> name=NC_010473:3533791..3534201 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3436046

Edman degradation

PMID:7511799[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmscL (Keio:JW3252)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10453[4]

ΔmscL727::kan

PMID:16738554[3]

CGSC:101954

mscL-K31C

-->TGC

K31C

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See Table 1

mscL-K31R

-->AGG

K31R

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental:AW405

See table 1

mscL-K31E

-->GAG

K31E

Sensitivity to

Activation pressure lowered by 18.3 mmHg

PMID:9635742[6]

Parental:AW405

See table 4

mscL-Q56P

-->CCT

Q56P

Sensitivity to

Pressure

PMID:8876191[5]

Parental:AW405

see table 1

mscL-Q56C

-->TGC

Q56C

Conductance

Increased channel open time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56F

-->TTT

Q56F

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56W

-->TGG

Q56W

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

see table 1

mscL-Q56Y

-->TAC

Q56Y

Conductance

Increased open channel time

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56L

-->TTA

Q56L

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56R

-->CGT

Q56R

Sensitivity to

Activation pressure lowered by 17.9 mmHg

PMID:9635742[6]

Parental: AW405

See table 4

mscLΔ2-4

Deletion

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See table 1

mscL-ΔQ56

Deletion

Sensitivity to

Pressure

PMID:8876191[5]

Parental: AW405

See table 1

mscLΔ110-136

Deletion

No significant change in pressure sensitivity or open time.

PMID:8876191[5]

Parental: AW405

See table 1

mscL-Q56H

-->CAT

Q56H

Sensitivity to

Increased sensitivity to pressure at pH 5 (activation pressure lowered by 18.6 mmHg) and increased open channel time at pH 7.5.

PMID:8876191[5] PMID:9635742[6]

Parental: AW405

See table 1 and table 4

mscL-K31D

-->GAT

K31D

Growth Phenotype

Inhibits growth

PMID:9405414[7]

See figure 1

mscL-K31E

K31E

Growth Phenotype

Inhibits growth

PMID:9405414[7]

See figure 1

mscLΔ104-136

Deletion

Conductance

Failed to exhibit functional channel

PMID:9141355[8]

See table 2

mscLΔEV

Internal deletion (Δ41-61)

Conductance

Failed to show functional channels

PMID:9141355[8]

See table 2

mscLNBE

Deletion (Δ1-8)

Conductance

Decreased conductance

PMID:9141355[8]

See table 2

mscL-G22R

G22R

Growth Phenotype

Growth inhibited and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22K

G22K

Growth Phenotype

Growth inhibited and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22D

G22D

Growth Phenotype

Growth inhibited and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22E

G22D

Growth Phenotype

Growth inhibited and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22S

G22S

Growth Phenotype

Growth decreased and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22Y

G22Y

Growth Phenotype

Growth decreased

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1

mscL-G22Q

G22Q

Growth Phenotype

Growth decreased and pressure threshold lowered.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22T

G22T

Growth Phenotype

Growth decreased and pressure threshold lowered

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22V

G22V

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1

mscL-G22L

G22L

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22W

G22W

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22F

G22F

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G11I

G22I

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22M

G22M

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22C

G22C

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

mscL-G22P

G22P

Resistant to

Pressure threshold increased. No change in growth.

PMID:10512816[9]

Parental plasmid: pb10b

See figure 1a and table 1.

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Notes

Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3252

Plasmid clone

Shigen

PMID:16769691[10]

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[4]

est. P1 cotransduction: 78% [11]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 2% [11]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11180

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11180

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001162

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947787

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1167

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010790

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Sukharev, SI et al. (1994) A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature 368 265-8 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 Blount, P et al. (1996) Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 11652-7 PubMed
  6. ↑ 6.0 6.1 6.2 Gu, L et al. (1998) Electromechanical coupling model of gating the large mechanosensitive ion channel (MscL) of Escherichia coli by mechanical force. Biophys. J. 74 2889-902 PubMed
  7. ↑ 7.0 7.1 Blount, P et al. (1997) Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress. J. Biol. Chem. 272 32150-7 PubMed
  8. ↑ 8.0 8.1 8.2 Häse, CC et al. (1997) Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity. J. Membr. Biol. 157 17-25 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 Yoshimura, K et al. (1999) Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys. J. 77 1960-72 PubMed
  10. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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