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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
|}
[[Category:Gene List:MG1655]][[Category:Gene List:W3110]][[Category:Genes in the Keio knockout collection]][[Category:Genes in the ASKA clone collection]]
</noinclude><protect>
</noinclude><protect>
==Nomenclature==
==Nomenclature==
{{BacktoTop}}
{{BacktoTop}}
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{|   id="Y46a9e4f168029"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''mutS''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''mutS''
Mutator
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK2728, b2733, JW2703, fdv, ant, plm''<ref name='LIB:Riley_2006'/>, plm''


|-
|- class="tableEdit_footer"
!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16031.Y46a9e4f168029&page=16031&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK2728, b2733, JW2703, fdv, ant, plm''<ref name='LIB:Riley_2006'/>
|-
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===Notes===
===Notes===
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<protect>
<protect>
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z46a9e4f1e731c"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
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61.54 minutes&nbsp;
61.54 minutes&nbsp;
|
|
MG1655: 2855115..2857676<br />{{GBrowseFigureByCoord|2854115|2858676|ecoli|MG1655}}
MG1655: 2855115..2857676<br /><gbrowseImage>
name=NC_000913:2855115..2857676
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10625 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10625 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:2854115..2858676 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|2854115|2858676|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:2855115..2857676&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|2855115|2857676|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10625 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10625 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 2751953..2754514<br /><gbrowseImage>
name=NC_012967:2751953..2754514
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|2751953|2754514|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2751953|2754514|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 2740927..2743488<br /><gbrowseImage>
name=NC_012759:2740927..2743488
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|2740927|2743488|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2740927|2743488|FASTA Dump}}
|-  
|
|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 2855749..2858310<br />{{GBrowseFigureByCoord|2854749|2859310|ecoli_W3110|W3110}}
W3110: 2855749..2858310<br /><gbrowseImage>
name=NC_007779:2855749..2858310
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:2854749..2859310 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|2854749|2859310|GBrowse(W3110)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:2855749..2858310&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|2855749|2858310|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 2947657..2950218<br /><gbrowseImage>
name=NC_010473:2947657..2950218
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|2942535|2955340|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|2942535|2955340|FASTA Dump}}
 
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9e4f2e8b0f"  class=" tableEdit Gene_allele_table dataTable" 
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|-  
|
|
&Delta;mutS (Keio:JW2703)
''mutS(del)'' (Keio:JW2703)
|
|
deletion
deletion
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|
|
[[PMID:1673855]]<ref name=PMID:1673855/>
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=108407 CGSC10126]<ref name='LIB:cgsc'/>
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0008977 mutS::Tn5KAN-I-SceI] (FB20917)
|
Insertion at nt 2340 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20917]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0008977 mutS::Tn5KAN-I-SceI] (FB20918)
|
Insertion at nt 2340 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20918]
|
does not contain [[pKD46]]
|-
|
''mutS201::Tn5''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:7794|CGSC:7794]]
|
|-
|
''mutS215::Tn10''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:7797|CGSC:7797]]
|
|-
|
''mutS3''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:11134|CGSC:11134]]
|
|-
|
''mutS210''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:85420|CGSC:85420]]
|
|-
|
''mutS104::mini-Tn10''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:85448|CGSC:85448]]
|
|-
|
''mutS738(del)::kan''
|
 
|
 
|
 
|
 
|
PMID:16738554
|
[[cgscmutation:101976|CGSC:101976]]
|
|-
|
''MutS F596A''
|
 
|
F596A
|
 
|
* Mildly defective ATPase activity
* Defective MutH activation ''in vitro''
* Moderately elevated mutation and DNA recombination rates ''in vivo''
|
PMID:12606120
|
 
|
|-
|
''MutS H760A''
|
 
|
H760A
|
 
|
* Mildly defective ATPase activity
* Defective MutH activation ''in vitro''
* Moderately elevated mutation and DNA recombination rates ''in vivo''
 
 
|
PMID:12606120
|
 
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
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|
''MutS H728A''
|
 
|
H728A
|
 
|
* Mildly defective ATPase activity
* Defective MutH activation ''in vitro''
* Moderately elevated mutation and DNA recombination rates ''in vivo''
 
|
PMID:12606120
|
 
|
|-
|
MutS S668A/T669A
|
 
|
S668A/T669A
|
 
|
*Modest reduction of K<sub>cat</sub> (~three-fold)
*Fails to activate MMR
|
PMID:12606120
|
 
|
|-
|
MutS D693N
|
 
|
D693N
|
 
|
*Modest reduction of K<sub>cat</sub> (~three-fold)
*Fails to activate MMR
|
PMID:12606120
|
 
|
|-
|
MutS S668A/T669A/E694A
|
 
|
S668A/T669A/E694A
|
 
|
*Defective in DNA binding
*Defective in MMR
*Catalyzes ATP hydrolysis better than single E694A mutant
|
PMID:12606120
|
 
|
|-
|
MutS K620M
|
 
|
K620M
|
 
|
*Severely reduced ATP binding capacity
*Fails to activate MutH in MMR initiation assays
|
PMID:12606120
|
 
|
|-
|
MutS E694Q
|
 
|
E694Q
|
 
|
*Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
*Supports low-level MutH activation in a mismatch-independent manner
|
PMID:12606120
|
 
|
|-
|
MutS F36A
|
 
|
F36A
|
 
|
*Fails to recognize a mismatch site hence failing to initiate MMR
*Loses mismatch binding capacity
|
PMID:12606120
|
 
|
|-
|
MutS D693A
|
 
|
D693A
|
 
|
*Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
*Supports low-level MutH activation in a mismatch-independent manner
|
PMID:12606120
|
 
|
|-
|
MutS E38Q
|
 
|
E38Q
|
 
|
*Binds DNA better than wt MutS
*Activates MutH ~50% more than the wt MutS in correlation with increased affinity for heteroduplex DNA
*Completely inactive in MMR and in preventing homologous recombination ''in vivo''
|
PMID:12606120
|
 
|
|-
|
MutS E694A
|
 
|
E694A
|
 
|
*Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
*Supports low-level MutH activation in a mismatch-independent manner
|
PMID:12606120
|
 
|
|-
|
MutS E38A
|
 
|
E38A
|
 
|
*Exhibits moderately increased affinity for homoduplex DNA ''in vitro''
*MMR defective ''in vivo''
|
PMID:12606120
|
 
|
|-
|
MutS E177A
|
 
|
E177A
|
 
|
*Unable to form H bonds that link domains I and II
*Indistinguishable from Δ''mutS'' in the ''in vivo'' MMR and recombination assays
*Possesses a lower affinity for heteroduplex DNA ''in vitro'' in the presence of ATP
*Possesses a higher affinity for homoduplex DNA ''in vitro'' in the presence of ATP
*Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
*Reduced ATP hydrolysis
|
PMID:12606120
|
 
|
|-
|
MutS T115A
|
 
|
T115A
|
 
|
*Unable to form H bonds that link domains I and II
*Indistinguishable from Δ''mutS'' in the ''in vivo'' MMR and recombination assays
*Possesses a lower affinity for heteroduplex DNA ''in vitro'' in the presence of ATP
*Possesses a higher affinity for homoduplex DNA ''in vitro'' in the presence of ATP
*Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
*Reduced ATP hydrolysis
|
PMID:12606120
|
 
|
|-
|
MutS R197E/R198E
|
 
|
R197E/R198E
|
 
|
*Disrupts the domain interface more extensively than the E177A or T115A mutation
*''In vivo'' defects similar to E177A and T115A mutations
*Relatively normal DNA binding
*Slightly elevated affinity for homoduplex DNA in the presence of ATP
*Completely fails to activate MutH ''in vitro''
|
PMID:12606120
|
 
|
|-
|
MutS
|
Overexpression of MutS
|
 
|
Mutation Frequence
|
The rate of GC transversion to TA is reduced.  
|
PMID 10940054
|
 
|
See Fig 1
|-
|
MutS R197E/R198E/R199E
|
 
|
R197E/R198E/R199E
|
 
|
*Disrupts the domain interface more extensively than the E177A or T115A mutation
*''In vivo'' defects similar to E177A and T115A mutations
*Probably results in an unstable protein, which is found in inclusion bodies when over-expressed
 
|
PMID:12606120
|
 
|
|-
|
MutS
|
Overexpression of MutS
|
 
|
Mutation Frequence
|
the amount of mutations due to MutY are decreased.  
|
PMID 10940054
|
 
|
See Fig 3
 
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
{{BacktoTop}}
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|- {{table_heading_style}}
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW2703
JW2703
Line 153: Line 763:
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 160: Line 770:


Primer 2:CCaACCAGGCTCTTCAAGCGATA  
Primer 2:CCaACCAGGCTCTTCAAGCGATA  
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[[:Category:Kohara_Phage:12F2|12F2]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=449 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:25D2|25D2]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=450 Genobase]
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PMID:3038334
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[[srlD:Gene|srlD3131::Tn10]]
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Linked marker
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CAG18642 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40097 CGSC7423]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 32% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
|-
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[[cysI:Gene|cysI95::Tn10]]
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Linked marker
|
CAG12173 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40099 CGSC7425]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 30% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:cysC95::Tn10
 
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===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
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|-  
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10625 EcoCyc:EG10625] 
EcoCyc:EG10625
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0620 EchoBASE:EB0620] 
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|-
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b2733 EcoliGenExpDB:b2733] 
EcoGene:EG10625
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C3294 ECOL199310CYC:C3294] 
RegulonDB:ECK120000618
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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|-  
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10625 EcoGene:EG10625]
[http://www.ncbi.nlm.nih.gov/gene?term=947206 GeneID:947206]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=MUTS ECOO157CYC:MUTS] 
EchoBASE:EB0620
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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|
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/469 CGSC:469] 
ASAP:ABE-0008977
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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__NOTOC__
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Gene_List:DH10B]]
[[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:mut genes]]

Latest revision as of 20:44, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutS

Mnemonic

Mutator

Synonyms

ECK2728, b2733, JW2703, fdv, ant, plm[1], plm

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

61.54 minutes 

MG1655: 2855115..2857676
<gbrowseImage> name=NC_000913:2855115..2857676 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2751953..2754514
<gbrowseImage> name=NC_012967:2751953..2754514 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2740927..2743488
<gbrowseImage> name=NC_012759:2740927..2743488 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2855749..2858310
<gbrowseImage> name=NC_007779:2855749..2858310 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2947657..2950218
<gbrowseImage> name=NC_010473:2947657..2950218 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mutS(del) (Keio:JW2703)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10126[3]

mutS::Tn5KAN-I-SceI (FB20917)

Insertion at nt 2340 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20917

contains pKD46

mutS::Tn5KAN-I-SceI (FB20918)

Insertion at nt 2340 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20918

does not contain pKD46

mutS201::Tn5

CGSC:7794

mutS215::Tn10

CGSC:7797

mutS3

CGSC:11134

mutS210

CGSC:85420

mutS104::mini-Tn10

CGSC:85448

mutS738(del)::kan

PMID:16738554[2]

CGSC:101976

MutS F596A

F596A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120[5]

MutS H760A

H760A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120[5]

MutS H728A

H728A

  • Mildly defective ATPase activity
  • Defective MutH activation in vitro
  • Moderately elevated mutation and DNA recombination rates in vivo

PMID:12606120[5]

MutS S668A/T669A

S668A/T669A

  • Modest reduction of Kcat (~three-fold)
  • Fails to activate MMR

PMID:12606120[5]

MutS D693N

D693N

  • Modest reduction of Kcat (~three-fold)
  • Fails to activate MMR

PMID:12606120[5]

MutS S668A/T669A/E694A

S668A/T669A/E694A

  • Defective in DNA binding
  • Defective in MMR
  • Catalyzes ATP hydrolysis better than single E694A mutant

PMID:12606120[5]

MutS K620M

K620M

  • Severely reduced ATP binding capacity
  • Fails to activate MutH in MMR initiation assays

PMID:12606120[5]

MutS E694Q

E694Q

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120[5]

MutS F36A

F36A

  • Fails to recognize a mismatch site hence failing to initiate MMR
  • Loses mismatch binding capacity

PMID:12606120[5]

MutS D693A

D693A

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120[5]

MutS E38Q

E38Q

  • Binds DNA better than wt MutS
  • Activates MutH ~50% more than the wt MutS in correlation with increased affinity for heteroduplex DNA
  • Completely inactive in MMR and in preventing homologous recombination in vivo

PMID:12606120[5]

MutS E694A

E694A

  • Binds ATP better than the wt MutS,but hydrolyzes ATP poorly
  • Supports low-level MutH activation in a mismatch-independent manner

PMID:12606120[5]

MutS E38A

E38A

  • Exhibits moderately increased affinity for homoduplex DNA in vitro
  • MMR defective in vivo

PMID:12606120[5]

MutS E177A

E177A

  • Unable to form H bonds that link domains I and II
  • Indistinguishable from ΔmutS in the in vivo MMR and recombination assays
  • Possesses a lower affinity for heteroduplex DNA in vitro in the presence of ATP
  • Possesses a higher affinity for homoduplex DNA in vitro in the presence of ATP
  • Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
  • Reduced ATP hydrolysis

PMID:12606120[5]

MutS T115A

T115A

  • Unable to form H bonds that link domains I and II
  • Indistinguishable from ΔmutS in the in vivo MMR and recombination assays
  • Possesses a lower affinity for heteroduplex DNA in vitro in the presence of ATP
  • Possesses a higher affinity for homoduplex DNA in vitro in the presence of ATP
  • Hyperactive in activating MutH with 2-fold and 4-fold increases observed relative to the wt MutS for heteroduplex and homoduplex DNA, respectively
  • Reduced ATP hydrolysis

PMID:12606120[5]

MutS R197E/R198E

R197E/R198E

  • Disrupts the domain interface more extensively than the E177A or T115A mutation
  • In vivo defects similar to E177A and T115A mutations
  • Relatively normal DNA binding
  • Slightly elevated affinity for homoduplex DNA in the presence of ATP
  • Completely fails to activate MutH in vitro

PMID:12606120[5]

MutS

Overexpression of MutS

Mutation Frequence

The rate of GC transversion to TA is reduced.

PMID 10940054

See Fig 1

MutS R197E/R198E/R199E

R197E/R198E/R199E

  • Disrupts the domain interface more extensively than the E177A or T115A mutation
  • In vivo defects similar to E177A and T115A mutations
  • Probably results in an unstable protein, which is found in inclusion bodies when over-expressed

PMID:12606120[5]

MutS

Overexpression of MutS

Mutation Frequence

the amount of mutations due to MutY are decreased.

PMID 10940054

See Fig 3

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2703

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGTGCAATAGAAAATTTCGA

Primer 2:CCaACCAGGCTCTTCAAGCGATA

12F2

Kohara Phage

Genobase

PMID:3038334[7]

25D2

Kohara Phage

Genobase

PMID:3038334[7]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[3]

est. P1 cotransduction: 32% [8]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[3]

est. P1 cotransduction: 30% [8]
Synonyms:cysC95::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10625

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000618

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947206

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0620

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008977

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 Junop, MS et al. (2003) In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination. DNA Repair (Amst.) 2 387-405 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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