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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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W3110: 3037478..3036768<br /><gbrowseImage>
W3110: 3037478..3036768<br /><gbrowseImage>
name=AC_000091:3036768..3037478
name=NC_007779:3036768..3037478
source=W3110
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{{GBrowseLink|W3110|AC_000091|3035768|3038478|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|3035768|3038478|GBrowse(W3110)}}  
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{{GBrowseFastaLink|W3110|AC_000091|3036768|3037478|FASTA Dump}}  
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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The mutation caused hypersensitivity toward Benzylpenicillin  
The mutation caused hypersensitivity toward Benzylpenicillin  
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''dsbC''::Tn''10''
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Insertion
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Growth Phenotype
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Decreased Growth
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PMID:8168498
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The mutation caused a reduction of growth on minimal medium. 
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''dsbC13''::Tn''10''
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Insertion
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Disulfide Bond Formation
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Defective Disulfide Bond Formation
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PMID:8168498
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Defective Disulfide Bond formation. See figure 2 for experimental results.


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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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!|Resource!!Resource Type!!Source!!Notes/Reference
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG11070
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11070 ECOCYC:EG11070]
EcoGene:EG11070
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11070 EcoGene:EG11070]
RegulonDB:ECK120001059
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB1063
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0009494
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:44, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbC

Mnemonic

Disulfide bond

Synonyms

ECK2888, b2893, JW2861, xprA[1], xprA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

65.44 minutes 

MG1655: 3036844..3036134
<gbrowseImage> name=NC_000913:3036134..3036844 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2924575..2923865
<gbrowseImage> name=NC_012967:2923865..2924575 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2923282..2923992
<gbrowseImage> name=NC_012759:2923282..2923992 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3037478..3036768
<gbrowseImage> name=NC_007779:3036768..3037478 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3130714..3130004
<gbrowseImage> name=NC_010473:3130004..3130714 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3036137

Edman degradation

PMID:9298646[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dsbC(del) (Keio:JW2861)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8322[4]

dsbCC118T,A

C118T,A

Loss of activity

seeded from UniProt:P0AEG6

dsbCC121A

C121A

Partial loss of activity

seeded from UniProt:P0AEG6

dsbCC121V

C121V

Loss of activity

seeded from UniProt:P0AEG6

dsbCC161S

C161S

Destabilization of protein

seeded from UniProt:P0AEG6

dsbCC183L

C183L

Destabilization of protein

seeded from UniProt:P0AEG6

dsbC3::Cm

PMID:8917542[5] PMID:9352906[6]

CGSC:65870

dsbC744(del)::kan

PMID:16738554[3]

CGSC:100652

dsbC::Tn10

Insertion

Sensitivity to

Dithiothreitol

PMID:8168498[7]

The mutation conferred an increase toward Dithiothreitol

dsbC::Tn10

Insertion

Alkaline phosphate activity

Decreased alkaline phosphatase activity

PMID:8168498[7]

The mutation conferred a reduction of alkaline phosphatase activity.

dsbC::Tn10

Insertion

Sensitivity to

Benzylpenicillin sensitivity

PMID:8168498[7]

The mutation caused hypersensitivity toward Benzylpenicillin

dsbC::Tn10

Insertion

Growth Phenotype

Decreased Growth

PMID:8168498[7]

The mutation caused a reduction of growth on minimal medium.

dsbC13::Tn10

Insertion

Disulfide Bond Formation

Defective Disulfide Bond Formation

PMID:8168498[7]

Defective Disulfide Bond formation. See figure 2 for experimental results.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2861

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAGAAAGGTTTTATGTTGTT

Primer 2:CCTTTACCGCTGGTCATTTTTTG

22H4

Kohara Phage

Genobase

PMID:3038334[9]

5E11

Kohara Phage

Genobase

PMID:3038334[9]

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[4]

est. P1 cotransduction: % [10]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[4]

est. P1 cotransduction: 10% [10]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11070

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001059

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947363

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1063

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009494

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Rietsch, A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 13048-53 PubMed
  6. ↑ Rietsch, A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179 6602-8 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 Missiakas, D et al. (1994) The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 13 2013-20 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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